植物研究 ›› 2023, Vol. 43 ›› Issue (3): 370-378.doi: 10.7525/j.issn.1673-5102.2023.03.006
收稿日期:
2023-03-10
出版日期:
2023-05-20
发布日期:
2023-06-06
通讯作者:
张鹏
E-mail:zhangpeng@nefu.edu.cn
作者简介:
矫春晶(1998—),女,硕士研究生,主要从事森林培育研究。
基金资助:
Chunjing JIAO, Mingyue LI, Peng ZHANG()
Received:
2023-03-10
Online:
2023-05-20
Published:
2023-06-06
Contact:
Peng ZHANG
E-mail:zhangpeng@nefu.edu.cn
About author:
E-mail:zhangpeng@nefu.edu.cnSupported by:
摘要:
为了探究外源激素浸种与渗透处理联用对水曲柳(Fraxinus mandshurica)种子热休眠及相关生理变化的影响,以解除初生休眠的水曲柳种子为材料,分别用1 mmol·L-1赤霉素(GA4+7)、乙烯利(ETH)、细胞分裂素 (6-BA)和水浸种24 h,比较在诱导热休眠温度(25 ℃)下不同质量分数(0、5%、25%、50%)聚乙二醇(PEG)溶液中培养7 d后的种子在适温(10 ℃)下的萌发情况。在此基础上选择结果显著的处理测定种子内源激素含量和细胞壁降解酶活力的变化。结果表明:与单独激素处理相比,GA4+7与不同质量分数PEG联用对种子发芽能力影响不显著,6-BA与PEG联用处理的种子发芽能力随PEG质量分数的升高逐渐提高后趋于稳定,与25% PEG联用的种子发芽率和发芽指数分别提高69.31%和 1.04,发芽时间缩短7.8 d,ETH与PEG联用处理的种子发芽能力随PEG质量分数升高逐渐提高,与50% PEG联用的种子发芽率和发芽指数分别提高43.43%和0.76;联用后的种子胚乳纤维素酶活力显著提高,种胚和胚乳GA、ABA含量变化无显著规律。由此可知,外源激素与渗透处理联用减弱水曲柳种子热休眠的作用因激素种类而异。
中图分类号:
矫春晶, 李明月, 张鹏. 外源激素浸种与渗透处理对水曲柳种子热休眠的作用[J]. 植物研究, 2023, 43(3): 370-378.
Chunjing JIAO, Mingyue LI, Peng ZHANG. Effects of Exogenous Hormones Soaking and Osmotic Treatment on Thermal Dormancy of Seeds of Fraxinus mandshurica[J]. Bulletin of Botanical Research, 2023, 43(3): 370-378.
表2
不同外源激素浸种与PEG渗透处理后的水曲柳种子萌发情况
PEG质量分数 PEG mass fraction /% | 激素 Hormones | 发芽率 Germination percentage /% | 发芽指数 Germination index | 平均发芽时间 Germination time /d |
---|---|---|---|---|
0 | 对照(水) | 6.13±3.03g | 0.14±0.06h | 13.13±0.89f |
GA4+7 | 82.51±2.96a | 1.34±0.13ab | 16.91±0.85de | |
6-BA | 21.98±2.29f | 0.21±0.02gh | 25.88±0.96a | |
ETH | 9.00±3.00fg | 0.17±0.05gh | 14.35±1.63ef | |
5 | 对照 | 13.70±3.52fg | 0.22±0.06gh | 15.93±1.02def |
GA4+7 | 75.27±2.77ab | 1.09±0.76abcd | 18.42±0.98cd | |
6-BA | 68.05±5.15bc | 0.78±0.05cdef | 22.04±0.38b | |
ETH | 15.29±4.57fg | 0.28±0.08gh | 13.48±0.58f | |
25 | 对照 | 32.83±9.80de | 0.52±0.18fg | 16.39±1.29de |
GA4+7 | 88.56±4.96a | 1.43±0.14a | 16.65±0.54de | |
6-BA | 91.29±1.85a | 1.25±0.09abc | 18.08±0.73cd | |
ETH | 44.96±2.52d | 0.71±0.20ef | 15.73±0.35def | |
50 | 对照 | 59.39±8.46cd | 0.94±0.16cdef | 16.14±0.35de |
GA4+7 | 83.42±1.31ab | 1.06±0.03bcde | 19.93±0.49bc | |
6-BA | 89.12±1.58ab | 1.24±0.04abc | 17.99±0.38cd | |
ETH | 52.42±8.30cd | 0.93±0.15cde | 14.26±0.28ef |
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