Resistance of plant diversity to road disturbance: Evidence from the Tibetan Plateau

doi:10.1016/j.pld.2024.09.006

Abstract

Abstract: The expansion of road networks in recent decades has drawn considerable attention due to its impact on biodiversity in high-altitude ecosystems. Here, we conducted a comprehensive field survey to investigate the effects of road disturbance on plant diversity in alpine grasslands on the Tibetan Plateau. Our results indicate that road disturbance caused no significant changes in species richness, Shannon-Wiener’s diversity, or Simpson’s diversity, and the alteration in species composition was limited. These findings demonstrate the robust resistance of alpine grassland plant diversity to road disturbance. Plant diversity exhibited more resistance to road disturbance in regions with more hostile environments, such as plateau sub-frigid regions and alpine steppes. Our study suggests that road construction in the Tibetan Plateau poses limited risk to plant biodiversity.

Key words: Biodiversity, Highway, Anthropogenic disturbance, Stability, Resilience, Sustainability

摘要： The expansion of road networks in recent decades has drawn considerable attention due to its impact on biodiversity in high-altitude ecosystems. Here, we conducted a comprehensive field survey to investigate the effects of road disturbance on plant diversity in alpine grasslands on the Tibetan Plateau. Our results indicate that road disturbance caused no significant changes in species richness, Shannon-Wiener’s diversity, or Simpson’s diversity, and the alteration in species composition was limited. These findings demonstrate the robust resistance of alpine grassland plant diversity to road disturbance. Plant diversity exhibited more resistance to road disturbance in regions with more hostile environments, such as plateau sub-frigid regions and alpine steppes. Our study suggests that road construction in the Tibetan Plateau poses limited risk to plant biodiversity.

关键词: Biodiversity, Highway, Anthropogenic disturbance, Stability, Resilience, Sustainability

Ziwei Chen, Dongsheng Zhao, Siqi Deng, Yu Zhu, Ke Wang, Shunsheng Wang, Du Zheng. Resistance of plant diversity to road disturbance: Evidence from the Tibetan Plateau[J]. Plant Diversity, 2025, 47(03): 394-402.

References

Alpert, P., Bone, E., Holzapfel, C., 2000. Invasiveness, invasibility and the role of environmental stress in the spread of non-native plants. Perspect. Plant Ecol. Evol. Systemat. 3, 52-66. https://doi.org/10.1078/1433-8319-00004.
Ansong, M., Pickering, C., 2013. Are weeds hitchhiking a ride on your car? A systematic review of seed dispersal on cars. PLoS One 8, e80275. https://doi.org/10.1371/journal.pone.0080275.
Bai, X., Zhao, W., Wang, J., et al., 2022. Reducing plant community variability and improving resilience for sustainable restoration of temperate grassland. Environ. Res. 207, 112149. https://doi.org/10.1016/j.envres.2021.112149.
Bao, W., Zhang, W., Dong, J., et al., 2024. Impact of road corridors on soil properties and plant communities in high-elevation fragile ecosystems. Eur. J. For. Res. https://doi.org/10.1007/s10342-024-01720-x.
Barton, K., 2013. MuMIn: Multi-Model Inference.
Baselga, A., 2010. Partitioning the turnover and nestedness components of beta diversity. Global Ecol. Biogeogr. 19, 134-143. https://doi.org/10.1111/j.1466-8238.2009.00490.x.
Bates, D., 2010. Lme4: Mixed-Effects Modeling with R.
Bolter, M., Muller, F., 2016. Resilience in polar ecosystems: from drivers to impacts and changes. Pol. Sci. 10, 52-59. https://doi.org/10.1016/j.polar.2015.09.002.
CBD, 2020a. Global Biodiversity Outlook-5. Secretariat of the Convention on Biological Diversity, Montreal, Canada.
Chen, H., Li, S.-C., Zhang, Y.-L., 2003. Impact of road construction on vegetation alongside Qinghai-Xizang highway and railway. Chin. Geogr. Sci. 13, 340-346. https://doi.org/10.1007/s11769-003-0040-5.
Chen, H., Liu, J., Cao, Y., et al., 2007. Ecological risk assessment of regions along the roadside of the Qinghai-Tibet highway and railway based on an artificial neural network. Hum. Ecol. Risk Assess. 13, 900-913. https://doi.org/10.1080/10807030601105092.
Cheng, H., 2009. Study of Ecological Protection and Vegetation Restoration Technology at Ge-Tang Section of Qingzang Railway. Master, Hefei University of Technology.
Christen, D., Matlack, G., 2006. The role of roadsides in plant invasions: a demographic approach. Conserv. Biol. 20, 385-391. https://doi.org/10.1111/j.1523-1739.2006.00315.x.
Coffin, A.W., 2007. From roadkill to road ecology: a review of the ecological effects of roads. J. Transport Geogr. 15, 396-406. https://doi.org/10.1016/j.jtrangeo.2006.11.006.
Cole, A.J., Griffiths, R.I., Ward, S.E., et al., 2019. Grassland biodiversity restoration increases resistance of carbon fluxes to drought. J. Appl. Ecol. 56, 1806-1816. https://doi.org/10.1111/1365-2664.13402.
Cui, B., Zhao, S., Zhang, K., et al., 2009. Disturbance of Dabao highway construction on plant species and soil nutrients in longitudinal range gorge region (LRGR) of Southwestern China. Environ. Monit. Assess. 158, 545-559. https://doi.org/10.1007/s10661-008-0605-y.
Daniel-Ferreira, J., Fourcade, Y., Bommarco, R., et al., 2023. Communities in infrastructure habitats are species rich but only partly support species associated with semi-natural grasslands. J. Appl. Ecol. 60, 837-848. https://doi.org/10.1111/1365-2664.14378.
Ding, L., Kapp, P., Cai, F., et al., 2022. Timing and mechanisms of Tibetan Plateau uplift. Nat. Rev. Earth Environ. 3, 652-667. https://doi.org/10.1038/s43017-022-00318-4.
Feng, L., Li, X.-R., Zhang, J.-G., et al., 2012. Vegetation alteration in response to highway construction in the desertified steppe zone of the Tengger Desert, North China. Arid Land Res. Manag. 26, 59-78. https://doi.org/10.1080/15324982.2011.631690.
Fitch, G., Vaidya, C., 2021. Roads pose a significant barrier to bee movement, mediated by road size, traffic and bee identity. J. Appl. Ecol. 58, 1177-1186. https://doi.org/10.1111/1365-2664.13884.
Gao, X., Cao, X., Li, T., et al., 2019. Evolution of accessibility spatial pattern of the Qinghai-Tibet Plateau in 1976-2016. Acta Geograph. Sin. 74, 1190-1204. https://doi.org/10.11821/dlxb201906009.
Gardiner, M.M., Riley, C.B., Bommarco, R., et al., 2018. Rights-of-way: a potential conservation resource. Front. Ecol. Environ. 16, 149-158. https://doi.org/10.1002/fee.1778.
Gelbard, J.L., Harrison, S., 2003. Roadless habitats as refuges for native grasslands: interactions with soil, aspect, and grazing. Ecol. Appl. 13, 404-415. https://doi.org/10.1890/1051-0761(2003)013[0404:RHARFN]2.0.CO;2.
Guo, Z.G., Long, R.J., Niu, F.J., et al., 2007. Effect of highway construction on plant diversity of grassland communities in the permafrost regions of the Qinghai-Tibet Plateau. Rangel. J. 29, 161-167. https://doi.org/10.1071/RJ07016.
Gurka, M.J., 2006. Selecting the best linear mixed model under REML. Am. Statistician 60, 19-26. https://doi.org/10.1198/000313006X90396.
Hedges, L.V., Gurevitch, J., Curtis, P.S., 1999. The meta-analysis of response ratios in experimental ecology. Ecology 80, 1150-1156. https://doi.org/10.1890/0012-9658(1999)080.
Hillebrand, H., Bennett, D.M., Cadotte, M.W., 2008. Consequences of dominance: a review of evenness effects on local and regional ecosystem processes. Ecology 89, 1510-1520. https://doi.org/10.1890/07-1053.1.
Inger, A., Knut, R., Ingvild, A., 2011. Road verges: potential refuges for declining grassland species despite remnant vegetation dynamics. Ann. Bot. Fenn. 48, 289-303. https://doi.org/10.5735/085.048.0401.
IPBES, 2019. Global Assessment Report on Biodiversity and Ecosystem Services. Intergovernmental Science-Policy Platform on Biodiversity and Ecosystem Services, Bonn, Germany.
Jain, S., Shukla, S., Wadhvani, R., 2018. Dynamic selection of normalization techniques using data complexity measures. Expert Syst. Appl. 106, 252-262. https://doi.org/10.1016/j.eswa.2018.04.008.
Koleff, P., Gaston, K.J., Lennon, J.J., 2003. Measuring beta diversity for presence-absence data. J. Anim. Ecol. 72, 367-382. https://doi.org/10.1046/j.1365-2656.2003.00710.x.
Kreyling, J., Schweiger, A.H., Bahn, M., et al., 2018. To replicate, or not to replicate - that is the question: how to tackle nonlinear responses in ecological experiments. Ecol. Lett. 21, 1629-1638. https://doi.org/10.1111/ele.13134.
Laurance, W.F., Clements, G.R., Sloan, S., et al., 2014. A global strategy for road building. Nature 513, 229-232. https://doi.org/10.1038/nature13717.
Lauren, E.B., Kathryn, A.S., Melinda, D.S., 2018. Effects of feral horse herds on rangeland plant communities across a precipitation gradient. West. North Am. Nat. 77, 526-539. https://doi.org/10.3398/064.077.0412.
Lazaro-Lobo, A., Ervin, G.N., 2019. A global examination on the differential impacts of roadsides on native vs. exotic and weedy plant species. Glob. Ecol. Conserv. 17, e00555. https://doi.org/10.1016/j.gecco.2019.e00555.
Lembrechts, J.J., Milbau, A., Nijs, I., 2014. Alien roadside species more easily invade alpine than lowland plant communities in a subarctic mountain ecosystem. PLoS One 9, e89664. https://doi.org/10.1371/journal.pone.0089664.
Li, H., Luo, P., Yang, H., et al., 2022. Effect of road corridors on plant diversity in the Qionglai mountain range, China. Ecol. Indicat. 134, 108504. https://doi.org/10.1016/j.ecolind.2021.108504.
Liu, Z., Yang, Y., Ji, S., et al., 2021. Effects of elevation and distance from highway on the abundance and community structure of bacteria in soil along Qinghai-Tibet highway. Int. J. Environ. Res. Publ. Health 18, 13137. https://doi.org/10.3390/ijerph182413137.
Luo, J., Liu, X., Yang, J., et al., 2018. Variation in plant functional groups indicates land degradation on the Tibetan Plateau. Sci. Rep. 8, 17606. https://doi.org/10.1038/s41598-018-36028-5.
Myers, N., Mittermeier, R.A., Mittermeier, C.G., et al., 2000. Biodiversity hotspots for conservation priorities. Nature 403, 853-858. https://doi.org/10.1038/35002501.
Newbold, T., Hudson, L.N., Arnell, A.P., et al., 2016. Has land use pushed terrestrial biodiversity beyond the planetary boundary? A global assessment. Science 353, 288-291. https://doi.org/10.1126/science.aaf2201.
O'Farrell, P.J., Milton, S.J., 2006. Road verge and rangeland plant communities in the Southern Karoo: exploring what influences diversity, dominance and cover. Biodivers. Conserv. 15, 921-938. https://doi.org/10.1007/s10531-004-3102-9.
Okimura, T., Koide, D., Mori, A.S., 2016. Differential processes underlying the roadside distributions of native and alien plant assemblages. Biodivers. Conserv. 25, 995-1009. https://doi.org/10.1007/s10531-016-1103-0.
Pauchard, A., Kueffer, C., Dietz, H., et al., 2009. Ain't no mountain high enough: plant invasions reaching new elevations. Front. Ecol. Environ. 7, 479-486. https://doi.org/10.1890/080072.
Pauchard, A., Milbau, A., Albihn, A., et al., 2016. Non-native and native organisms moving into high elevation and high latitude ecosystems in an era of climate change: new challenges for ecology and conservation. Biol. Invasions 18, 345-353. https://doi.org/10.1007/s10530-015-1025-x.
Petitpierre, B., McDougall, K., Seipel, T., et al., 2016. Will climate change increase the risk of plant invasions into mountains? Ecol. Appl. 26, 530-544. https://doi.org/10.1890/14-1871.
Pourrezaei, J., Khajeddin, S.J., Karimzadeh, H., et al., 2020. Effects of road features on the phytogeographical characteristics of plant species on natural-area roadsides. Folia Geobot. 55, 365-379. https://doi.org/10.1007/s12224-020-09382-4.
Pysek, P., Jarosik, V., Pergl, J., et al., 2011. Colonization of high altitudes by alien plants over the last two centuries. Proc. Natl. Acad. Sci. U.S.A. 108, 439-440. https://doi.org/10.1073/pnas.1017682108.
Ran, Q., Hao, Y., Xia, A., et al., 2019. Quantitative assessment of the impact of physical and anthropogenic factors on vegetation spatial-temporal variation in Northern Tibet. Rem. Sens. 11, 1183. https://doi.org/10.3390/rs11101183.
Ru, H., Xu, J., Jiang, S., 2022. Experimental observation and analysis of traffic impact on Tibetan antelopes on the Qinghai-Tibet highway. Adv. Civ. Eng. 2022, 1226781. https://doi.org/10.1155/2022/1226781.
Shen, H., Dong, S., DiTommaso, A., et al., 2024. Physiological factors contribute to increased competitiveness of grass relative to sedge, forb and legume species under different N application levels. Sci. Total Environ. 906, 167466. https://doi.org/10.1016/j.scitotenv.2023.167466.
Suarez-Esteban, A., Fahrig, L., Delibes, M., et al., 2016. Can anthropogenic linear gaps increase plant abundance and diversity? Landsc. Ecol. 31, 721-729. https://doi.org/10.1007/s10980-015-0329-7.
Sun, H., Niu, Y., Chen, Y.-S., et al., 2014. Survival and reproduction of plant species in the Qinghai-Tibet Plateau. J. Systemat. Evol. 52, 378-396. https://doi.org/10.1111/jse.12092.
Tan, Z., Chen, X., Wang, Y., et al., 2024. The impact of the Qinghai-Tibet highway on plant community and diversity. Front. Plant Sci. 15, 1392924. https://doi.org/10.3389/fpls.2024.1392924.
Truscott, A.M., Palmer, S.C.F., McGowan, G.M., et al., 2005. Vegetation composition of roadside verges in Scotland: the effects of nitrogen deposition, disturbance and management. Environ. Pollut. 136, 109-118. https://doi.org/10.1016/j.envpol.2004.12.009.
Vanneste, T., Govaert, S., De Kesel, W., et al., 2020. Plant diversity in hedgerows and road verges across Europe. J. Appl. Ecol. 57, 1244-1257. https://doi.org/10.1111/1365-2664.13620.
Von Der Lippe, M., Kowarik, I., 2007. Long-distance dispersal of plants by vehicles as a driver of plant invasions. Conserv. Biol. 21, 986-996. https://doi.org/10.1111/j.1523-1739.2007.00722.x.
Vorstenbosch, T., Essl, F., Lenzner, B., 2020. An uphill battle? The elevational distribution of alien plant species along rivers and roads in the Austrian Alps. NeoBiota 63, 1-24. https://doi.org/10.3897/neobiota.63.55096.
Wang, G., 2015. Study on Effect of Traffic on Roadside Soil Environment in Lahsa and the Surrounding Area. Master, Beijing Jiaotong University.
Wang, G., Yao, J., Guo, Z., et al., 2004. Changes in permafrost ecosystem under the influences of human engineering activities and its enlightenment to railway construction. Chin. Sci. Bull. 49, 1741-1750. https://doi.org/10.1007/BF03184309.
Wang, Y., Kong, Y., Zeng, Y., et al., 2014. Impact of 30 years operation of Karakorum Highway on roadside vegetation. World Sci-Tech R&D 36, 392-397. https://doi.org/10.3969/j.issn.1006-6055.2014.04.005.
Wang, Y., Li, G., Shi, Y., et al., 2023a. Analysis of relationship between soil seed bank and aboveground vegetation in low mountain area of Helan mountain. Acta Agrestia Sin. 31, 166-172. https://doi.org/10.11733/j.issn.1007-0435.2023.01.019.
Wang, Y., Lv, W., Xue, K., et al., 2022. Grassland changes and adaptive management on the Qinghai-Tibetan Plateau. Nat. Rev. Earth Environ. 3, 668-683. https://doi.org/10.1038/s43017-022-00330-8.
Wang, Y., Wang, C., Ren, F., et al., 2023b. Asymmetric response of aboveground and belowground temporal stability to nitrogen and phosphorus addition in a Tibetan alpine grassland. Global Change Biol. 29, 7072-7084. https://doi.org/10.1111/gcb.16967.
Yang, S., Zhang, L., Zhu, G., 2023. Effects of transport infrastructures and climate change on ecosystem services in the integrated transport corridor region of the Qinghai-Tibet Plateau. Sci. Total Environ. 885, 163961. https://doi.org/10.1016/j.scitotenv.2023.163961.
Zeng, S.-L., Zhang, T.-T., Gao, Y., et al., 2011. Effects of road age and distance on plant biodiversity: a case study in the Yellow River Delta of China. Plant Ecol. 212, 1213-1229. https://doi.org/10.1007/s11258-011-9899-x.
Zhang, H., Wang, Z., Zhang, Y., et al., 2015. Identification of traffic-related metals and the effects of different environments on their enrichment in roadside soils along the Qinghai-Tibet highway. Sci. Total Environ. 521-522, 160-172. https://doi.org/10.1016/j.scitotenv.2015.03.054.
Zhang, T., Qiao, Q., Novikova, P.Y., et al., 2019. Genome of Crucihimalaya himalaica, a close relative of Arabidopsis, shows ecological adaptation to high altitude. Proc. Natl. Acad. Sci. U.S.A.116, 7137-7146. https://doi.org/10.1073/pnas.1817580116.
Zhou, B., Wei, G., Zhang, Y., et al., 2022. Study on the diversities of thermal impacts of Qinghai-Tibet Highway on permafrost under different surface conditions. J. Glaciol. Geocryol. 44, 470-484. https://doi.org/10.7522/j.issn.1000-0240.2022.0052.
Zhou, D., Zhang, L., Huang, L., et al., 2023. Satellite evidence for small biophysical effects of transport infrastructure in the Qinghai-Tibet Plateau. J. Clean. Prod. 416, 138002. https://doi.org/10.1016/j.jclepro.2023.138002.
Zhou, J., Yang, J., Peng, G., 2008. Constructing a green railway on the Tibet Plateau: evaluating the effectiveness of mitigation measures. Transport. Res. Part D-Transp. Environ. 13, 369-376. https://doi.org/10.1016/j.trd.2008.06.002.
Zhou, T., Luo, X., Hou, Y., et al., 2020. Quantifying the effects of road width on roadside vegetation and soil conditions in forests. Landsc. Ecol. 35, 69-81. https://doi.org/10.1007/s10980-019-00930-8.
Zuur, A.F., Ieno, E.N., Walker, N.J., et al., 2009. Mixed Effect Models and Extensions in Ecology with R. Springer.

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