Abstract: Nutrient solution was added into soil to improve the growth of hyphae and its host seedlings ( Coffea arabica). Pure culture of Phlebopus portentosus was inoculated in the roots of the host seedling. Results showed that over 92.5% of seedlings were found with mycorrhiza . External hyphae develop to the top or side of the roots, and even in the soil around roots. Fungal colonies (shiros) were ultimately formed. It was found that young fruit bodies appeared around or among the host seedlings after inoculation of 20 days. 52 of 162 young fruit bodies grew into mature in pots.

Key words: Phlebopus portentosus

摘要： 在土壤中添加促进暗褐网柄牛肝菌( Phlebopus portentosus) 菌丝及其宿主咖啡生长的营养液, 用暗褐网柄牛肝菌纯培养种接种盆栽件下的咖啡苗根系, 结果表明: 92.5% 以上的小粒咖啡( Coffea arabica ) 苗形成菌根, 菌根上外延菌丝向根尖、侧根及根系周围的土壤延伸生长, 与土粒相互交结形成菌塘。接种20 天后, 菌塘中子实体紧贴咖啡苗茎基或于咖啡苗株间生长, 共生长162 个子实体, 发育成熟52 个。子实体菌柄基部菌索与咖啡苗主根表面菌套连接。

关键词: 暗褐网柄牛肝菌, 营养液, 人工菌塘, 成熟子实体