Asymbiotic Seed Germination and in vitro Seedling Rapid Development of Paphiopedilum spicerianum

doi:10.7677/ynzwyj201515012

Abstract

Abstract:

In this paper, we studied the asymbiotic seed germination and seedling development of Paphiopedilum spicerianum, a wild plant with extremely small populations (PSESP) in China. By analogizing the influences of different media, seed maturity, pretreatments and lights on the seed germination, the results showed that the best germination condition is using the seeds at 270 days after pollination, pretreating with 1% NaOCl for 40 min, on the 1/4MS+10% coconut water medium under 24 hours darkness for 4 weeks. For seedling formation, 30g·L-1 Hyponex No 1 medium with 10mg·L-1 αnaphthlene acetic acid, 05g·L-1 activated charcoal and 10% banana homogenate was the most effective. For seedling development, the same medium used for seedling formation with supplemented 10g·L-1 6benzyladenine was most suitable.

Key words: Paphiopedilum spicerianum, Asymbiotic seed germination, Wild Plants with Extremely Small Populations, Orchid conservation, in vitro seedling rapid production

摘要：

对我国极小种群野生植物白旗兜兰的种子非共生萌发与试管苗快速繁育开展研究，对比了不同培养基、预处理、种子成熟度及光照对种子萌发的影响。白旗兜兰种子萌发的最适条件是授粉后270d的种子，在1/4MS+10%椰汁培养基上，用1% NaOCl溶液处理种子40min并在播种后的前4周进行黑暗培养。最适合原球茎转化幼苗的培养基是30g·L-1花宝一号+10mg·L-1 NAA+05g·L-1活性炭+10%香蕉匀浆, 而最佳幼苗生根壮苗的培养基是30g·L-1花宝一号+10mg·L-1 NAA+10mg·L-1 6BA+05g·L-1活性炭+10%香蕉匀浆。

关键词: 白旗兜兰, 种子非共生萌发, 极小种群物种, 兰科植物保护, 试管苗快繁

CLC Number:

Q 945

CHEN Ying, FAN Xu-Li, GAO Jiang-Yun. Asymbiotic Seed Germination and in vitro Seedling Rapid Development of Paphiopedilum spicerianum[J]. Plant Diversity, 2015, 37(05): 611-615.

陈莹, 范旭丽, 高江云. 白旗兜兰种子非共生萌发与试管苗快速繁育[J]. Plant Diversity, 2015, 37(05): 611-615.

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