Integrative Biology Journals
Plant Diversity

Plant Diversity ›› 2007, Vol. 29 ›› Issue (05): 559-562.

• Articles • Previous Articles     Next Articles

Cultivation and Molecular Identification of Two Bolete Strains Isolated from Boletus edulis Complex of Yunnan, China

WANG Hai-Kun1, 2, LI Tao1, ZHAO Dan-Dan3, LI Ling-Fei1, SHA Tao1, ZHAO Zhi-Wei1

  

  1. 1 Key Laboratory for Conservation and Utilization of Bio-Resources, and Key Laboratory for Microbial Resources of Minitry of Education , Yunnan University , Kunming 650091 , China ; 2 Kunming Teachers College, Kunming 650031 , China; 3 Institute of Horticulture of Yunnan Academy of Agricultural Sciences, Kunming 650205 , China

  • Received:2007-03-05 Online:2007-10-25 Published:2007-10-25
  • Contact: ZHAO Zhi-Wei

两株滇产广义美味牛肝菌的分离培养及其分子鉴定

王海坤1, 2, 李 涛1, 赵丹丹3, 李凌飞1, 沙涛1, 赵之伟1   

  1. 1 云南大学生物资源保护与利用国家重点实验室培育基地, 微生物多样性可持续利用教育部重点实验室, 云南昆明 650091;
    2 昆明师范高等专科学校, 云南昆明 650031 ; 3 云南省农业科学院园艺作物研究所, 云南昆明 650205
  • 通讯作者: 赵之伟

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Abstract: Two bolete strains were obtained from fruiting bodies by tissue isolation and culture methods. Conditions for isolation and cultivation of the two strains were studied .Molecular identification was carried out based on sequence analysis of the internal transcribed spacers ( ITS1-5.8S-ITS2) of the strains. Neighbour-joining analysis showed that the two isolates belong to the Boletus edulis complex and have a close phylogenetic relationship to B. aestivalis.

Key words: Boletus edulis

摘要： 采用组织分离法从广义美味牛肝菌子实体分离获得2 株稳定的菌株, 初步研究了两菌株的分离和培养条件。用ITS 序列分析, 对分离菌株进行了分子鉴定。基于ITS 序列构建的系统树表明两株菌属于美味牛肝菌复合群, 并与夏生牛肝菌Boletus aestivalis (Paul .) Fr . 有较近的亲缘关系。

关键词: 美味牛肝菌, 分离培养, 分子鉴定

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