Abstract: The genetic diversity of Ligularia tongolensis ( Franch.) Hand.-Mazz. was analyzed by inter-simple sequence repeat markers (ISSR). Eight informative and reliable primers were chosen from100 pre-screened primers, of which, 148 ISSR loci were polymorphic. Genetic diversity was high with PPB = 50. 45%, H = 0.1595 and I = 0.2440 at population level; PPB = 88.10% , H = 0.2811 and I = 0.4279 at species level. The G_st = 0.4355 indicated a high degree of genetic differentiation occurred among L. tongolensis populations. The main factor responsible for the high level genetic diversity and genetic differentiation among populations ascribed to diversified habitats diversification. Gene flow was inhibited by its habitats discontinuity which finally caused the genetic differentiation .

Key words: Ligularia tongolensis

摘要： 应用ISSR 标记对东俄洛橐吾( Ligularia tongolensis) 的遗传多样性进行了研究。从100 个引物中筛选出8 个用于正式扩增。在所研究的8 个居群共150 个个体中检测到148 个多态位点。在居群水平上, 多态位点百分率( PPB) 为50.45% , Nei′s 基因多样性指数( H) 和Shannon 信息指数( I) 分别为0.1595 和0. 2440。在物种水平上, 多态位点百分率( PPB) 为88.10% , Nei′s 基因多样性指数( H) 和Shannon 信息指数( I )分别为0.2811 和0.4279。居群间的遗传分化系数( G_st ) 达0.4355。研究结果表明东俄洛橐吾的遗传多样性水平很高, 居群间遗传分化较大。这与其多样化的生态环境是有必然联系的。因适应其多样化的生态环境而形成了遗传多样性; 且因其生态环境的不连续性阻碍了居群间的基因交流而产生了遗传分化, 即东俄洛橐吾高水平的遗传多样性和遗传分化是适应其分布区多样化生态环境的结果。

关键词: 东俄洛橐吾, 居群, 遗传多样性, 遗传分化