Genetic Diversity Analysis of Nelumbo Accessions Based on ESTSSR Markers

doi:10.7677/ynzwyj201515004

Abstract

Abstract:

EST-SSR markers were applied to estimate the genetic diversity for 30 accessions of Nelumbo nucifera, 6 accessions of Nelumbo lutea and 14 hybrids between these two species. The 52 of 123 ESTSSR markers (423%) were screened and then applied to amplify the 50 Nelumbo accessions. A total of 177 alleles were identified, and the number of alleles per locus and Polymorphic Information Content (PIC) value varied from 2 to 8 with an average of 34 alleles and from 063 (NNFB1059) to 091 (NNFB750) with an average value of 079, respectively. Jaccard similarity coefficients of the amplification results were analyzed by NTSYSpc2.11 software and the genetic similarity coefficient was from 024 to 086. The clustering dendrogram constructed by UPGMA method indicated that 50 accessions of Nelumbo could be divided into four major groups at the similarity coefficient of 037. Group I and group II included Nnucifera; group III included the majority of AsianAmerican lotus hybrids; and group IV included Nlutea. In addition, the AsianAmerican hybrids were closer to Nnucifera based on genetic relationship, which is consistent with the traditional classification result and the previous reports.

Key words: Nelumbo, EST-SSR, Genetic diversity, Genetic relationship

摘要：

利用ESTSSR分子标记对30个亚洲莲、6个美洲莲及14个亚美杂交莲品种 (野生居群) 进行遗传多样性分析，结果表明：从123对ESTSSR引物中筛选出52对（423%）扩增稳定、具有多态性的引物；利用这52对引物对50个不同类型代表的莲属品种进行扩增，共获得177条多态性条带，引物的等位基因数和多态性信息量（PIC）的范围分别为2~8个和063 （NNFB1059） 091 （NNFB750)，平均为34个和079。利用NTSYSpc2.11软件对扩增结果进行Jaccard相似性系数分析，50个荷花品种材料遗传相似系数为024~086；通过UPGMA法进行聚类分析，在遗传相似系数037处供试荷花材料可分为4大类群：亚洲莲品种均聚类在I、II类群中，III类群绝大部分为亚美杂交莲品种，IV类群为美洲莲，亚美杂交莲品种与亚洲莲品种的亲缘关系相对较近，与传统分类及前人研究结果相一致。

关键词: 莲, EST-SSR, 遗传多样性, 亲缘关系

CLC Number:

Q 16

XU Yu-Xian, ZHANG Wei-Wei, MO Hai-Bo, LI Chun, CAO Jian-Guo, TIAN Dai-Ke. Genetic Diversity Analysis of Nelumbo Accessions Based on ESTSSR Markers[J]. Plant Diversity, 2015, 37(05): 595-604.

徐玉仙, 张微微, 莫海波, 李春, 曹建国, 田代科. 基于ESTSSR标记的莲属种质资源遗传多样性分析[J]. Plant Diversity, 2015, 37(05): 595-604.

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