Temperature is a cryptic factor shaping the geographical pattern of genetic variation in Ceratophyllum demersum across a subtropical freshwater lake

doi:10.1016/j.pld.2023.08.002

Plant Diversity ›› 2024, Vol. 46 ›› Issue (05): 630-639.DOI: 10.1016/j.pld.2023.08.002

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Temperature is a cryptic factor shaping the geographical pattern of genetic variation in Ceratophyllum demersum across a subtropical freshwater lake

Yixian Li^a,b, Xuyao Zhao^a, Manli Xia^a,b, Xinzeng Wei^b,c, Hongwei Hou^a,b

a. The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, Hubei, China;
b. University of Chinese Academy of Sciences, Beijing 100049, China;
c. Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, Hubei, China

Received:2023-05-07 Revised:2023-08-08 Online:2024-09-07 Published:2024-09-25
Contact: Hongwei Hou,E-mail:houhw@ihb.ac.cn
Supported by:
Sample collection was supported by Liangzi Lake reserve. The study was supported by the International Partnership Program of Chinese Academy of Sciences [Grant number, 152342KYSB20200021], the National Key R and D Program of China [Grant numbers, 2020YFD0900305, 2018YFD0900801], National Natural Science Foundation of China [Grant numbers, 32001107, 32201285, 32101254].

Temperature is a cryptic factor shaping the geographical pattern of genetic variation in Ceratophyllum demersum across a subtropical freshwater lake

Yixian Li^a,b, Xuyao Zhao^a, Manli Xia^a,b, Xinzeng Wei^b,c, Hongwei Hou^a,b

a. The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, Hubei, China;
b. University of Chinese Academy of Sciences, Beijing 100049, China;
c. Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, Hubei, China

通讯作者: Hongwei Hou,E-mail:houhw@ihb.ac.cn
基金资助:
Sample collection was supported by Liangzi Lake reserve. The study was supported by the International Partnership Program of Chinese Academy of Sciences [Grant number, 152342KYSB20200021], the National Key R and D Program of China [Grant numbers, 2020YFD0900305, 2018YFD0900801], National Natural Science Foundation of China [Grant numbers, 32001107, 32201285, 32101254].

Abstract

Abstract: Macrophyte habitats exhibit remarkable heterogeneity, encompassing the spatial variation of abiotic and biotic components such as changes in water conditions and weather as well as anthropogenic stressors. Environmental factors are thought to be important drivers shaping the genetic and epigenetic variation of aquatic plants. However, the links among genetic diversity, epigenetic variation, and environmental variables remain largely unclear, especially for clonal aquatic plants. Here, we performed population genetic and epigenetic analyses in conjunction with habitat discrimination to elucidate the environmental factors driving intraspecies genetic and epigenetic variation in hornwort (Ceratophyllum demersum) in a subtropical lake. Environmental factors were highly correlated with the genetic and epigenetic variation of C. demersum, with temperature being a key driver of the genetic variation. Lower temperature was detected to be correlated with greater genetic and epigenetic variation. Genetic and epigenetic variation were positively driven by water temperature, but were negatively affected by ambient air temperature. These findings indicate that the genetic and epigenetic variation of this clonal aquatic herb is not related to the geographic feature but is instead driven by environmental conditions, and demonstrate the effects of temperature on local genetic and epigenetic variation in aquatic systems.

Key words: Genetic diversity, Epigenetic variation, Temperature, Macrophyte, Restoration

摘要： Macrophyte habitats exhibit remarkable heterogeneity, encompassing the spatial variation of abiotic and biotic components such as changes in water conditions and weather as well as anthropogenic stressors. Environmental factors are thought to be important drivers shaping the genetic and epigenetic variation of aquatic plants. However, the links among genetic diversity, epigenetic variation, and environmental variables remain largely unclear, especially for clonal aquatic plants. Here, we performed population genetic and epigenetic analyses in conjunction with habitat discrimination to elucidate the environmental factors driving intraspecies genetic and epigenetic variation in hornwort (Ceratophyllum demersum) in a subtropical lake. Environmental factors were highly correlated with the genetic and epigenetic variation of C. demersum, with temperature being a key driver of the genetic variation. Lower temperature was detected to be correlated with greater genetic and epigenetic variation. Genetic and epigenetic variation were positively driven by water temperature, but were negatively affected by ambient air temperature. These findings indicate that the genetic and epigenetic variation of this clonal aquatic herb is not related to the geographic feature but is instead driven by environmental conditions, and demonstrate the effects of temperature on local genetic and epigenetic variation in aquatic systems.

关键词: Genetic diversity, Epigenetic variation, Temperature, Macrophyte, Restoration

Yixian Li, Xuyao Zhao, Manli Xia, Xinzeng Wei, Hongwei Hou. Temperature is a cryptic factor shaping the geographical pattern of genetic variation in Ceratophyllum demersum across a subtropical freshwater lake[J]. Plant Diversity, 2024, 46(05): 630-639.

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Temperature is a cryptic factor shaping the geographical pattern of genetic variation in Ceratophyllum demersum across a subtropical freshwater lake

Temperature is a cryptic factor shaping the geographical pattern of genetic variation in Ceratophyllum demersum across a subtropical freshwater lake

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