夏蜡梅遗传多样性迁地保护取样策略研究

Plant Diversity ›› 2010, Vol. 32 ›› Issue (S17): 74-80.

夏蜡梅遗传多样性迁地保护取样策略研究

陈香波1, 田旗2

1 上海市园林科学研究所，上海200232；2 上海辰山植物园，上海201602

出版日期:2010-12-25 发布日期:2011-01-28
基金资助:
国家环保部专项“中国重要观赏植物种质资源调查”（物种08二31）、上海市绿化和市容管理局项目“夏蜡梅引种及栽培应用技术研究”（G060208）

Study on the Sampling Strategy of Sinocalycanthus chinensis for Exsitu Conservation of Genetic Diversity

CHEN Xiang-Bo-1, TIAN Qi-2

1 Shanghai Landscape Gardening Research Institute, Shanghai 200232, China;
2 Chenshan Botanical Garden, Shanghai 201602, China

Online:2010-12-25 Published:2011-01-28
Supported by:
国家环保部专项“中国重要观赏植物种质资源调查”（物种08二31）、上海市绿化和市容管理局项目“夏蜡梅引种及栽培应用技术研究”（G060208）

摘要/Abstract

摘要：

采用ISSR分子标记技术分析夏蜡梅天然群体的遗传多样性及空间遗传结构并在此基础上利用计算机模拟取样，以保留95%以上天然群体等位基因为标准制定出夏蜡梅遗传多样性的取样策略，即采集6个群体，群体抽样比例为30%，居群取样时应适当控制采种间距在26 m以上。采种实施迁地保护后，后代群体检测证实该取样策略能够有效保留原始群体的遗传多样性，由此探讨了濒危植物系统迁地保护的采样模式与技术措施。

关键词: 夏蜡梅, 遗传多样性, 迁地保护, 取样策略

Abstract:

By analysis of genetic diversity and population genetic structure in natural populations of Sinocalycanthus chinensis with intersimple sequence repeat(ISSR) technique, the sampling strategy were determined based on the gene capture curve of 95% allele captured. The results are as follows: 6 populations to be sampled, the sampling proportion should be 30% and the distance between the sampled individuals are best of 26 m above. After seeds sampling and conduction of Exsitu conservation, the genetic diversity of the offspring population were evaluated that it conserved the genetic diversity of the natural populations effectively. Therefore, the systemic sampling method of endangered plants for Exsitu conservation of genetic diversity were discussed.

Key words: Sinocalycanthus chinensis, Genetic diversity, Exsitu conservation, Sampling strategy

中图分类号:

Q 16

陈香波1, 田旗2. 夏蜡梅遗传多样性迁地保护取样策略研究[J]. Plant Diversity, 2010, 32(S17): 74-80.

CHEN Xiang-Bo-, TIAN Qi. Study on the Sampling Strategy of Sinocalycanthus chinensis for Exsitu Conservation of Genetic Diversity[J]. Plant Diversity, 2010, 32(S17): 74-80.

参考文献

许再富, 1998. 稀有濒危植物迁地保护的原理与方法. 昆明: 云南科技出版社
李正宏, 2005. 濒危植物取样策略研究 \[D\]. 杭州: 浙江大学
赵冰, 2008. 蜡梅种质遗传多样性与核心种质构建的研究 \[D\]. 北京: 北京林业大学
Chen XB (陈香波), Ye WG (叶文国), Tian Q (田旗) et al, 2010. Phenotypic variation and distribution pattern of natural populations of Sinocalycanthus chinensis \[J\]. Journal of Beijing Forestry University (北京林业大学学报), 32 (2): 133—140
Doyle JJ, Doyle JL, 1990. Isolation of plant DNA from fresh tissue \[J\]. Focus, 12: 13—15
He JS (何敬胜), Li ZZ (李作洲), Huang HW (黄宏文), 2005. Spatial autocorrelation of allozyme genetic variation of the endangered Manglietia patungensis (Magnoliaceae) \[J\]. Acta Botanica Yunnanica(云南植物研究), 27(2): 171—180
Hedrick PW, Kalinowski ST, 2000. Inbreeding depression in conservation biology \[J\].Annual Review of Ecology & Sysematics, 31: 139—162
Jin Y (金燕), Lu BR (卢宝荣), 2003. Sampling strategy for genetic diversity \[J\].Biodiversity Science(生物多样性), 11 (2): 155—161
Jin Y, Zhang WJ, Fu DX et al, 2003. Sample stragety within a wild soybean population based on its genetic variation detected by ISSR markers \[J\].Acta Botanica Sinica(植物学报), 45 (8): 995—1002
Jin ZX (金则新), Li JM (李钧敏), 2007. ISSR analysis on genetic diversty of endangered relic shrub Sinocalycanthus chinensis \[J\].Chinese Journal of Applied Ecology(应用生态学报), 18 (2): 247—253
Kang M (康明), Ye QG (叶其刚), Huang HW (黄宏文), 2005. Genetic risks in plant exsitu conservation \[J\].Hereditas(遗传), 27 (1): 160—166
Li A (李昂), Luo YB (罗毅波), Ge S (葛颂), 2002. Spatial autocorrelation study of population genetic structure of two orchid species \[J\]. Biodiversity Science(生物多样性), 10 (3): 249—257
Li Q, Xu Z, He T, 2002.Exsitu genetic conservation of endangered Vatica guangxiensis (Diperocaropaceae) in China \[J\]. Biological Conservation, 106: 161—166
Li ZH (李正宏), Qiu YX (邱英雄), Xu HM (徐海明) et al, 2006. Study on the best sampling proportion for the core accessions of the endangered species by Monte Cario method \[J\].Journal of Zhejiang University(Agric

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