小桐子EST-SSR分子标记的开发与种质遗传多样性分析

doi:10.3724/SP.J.1143.2011.11064

Plant Diversity ›› 2011, Vol. 33 ›› Issue (5): 529-534.DOI: 10.3724/SP.J.1143.2011.11064

小桐子EST-SSR分子标记的开发与种质遗传多样性分析

杨春1、2, 刘爱忠1

1 中国科学院西双版纳热带植物园，云南勐腊666303； 2 中国科学院研究生院，北京100049

收稿日期:2011-04-18 出版日期:2011-10-25 发布日期:2011-05-19
基金资助:
国家自然科学基金项目(30871548)

Development of EST-SSR Markers from Jatropha curcas (Euphorbiaceae) and Their Application in Genetic Diversity Analysis among Germplasms

YANG Chun-1、2, LIU Ai-Zhong-1

1 Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla 666303, China;
2 Graduate University of Chinese Academy of Sciences, Beijing 100049, China

Received:2011-04-18 Online:2011-10-25 Published:2011-05-19
Supported by:
国家自然科学基金项目(30871548)

摘要/Abstract

摘要：

小桐子(Jatropha curcas) 适应性强，不择土壤，种子油脂性能适宜生物柴油的生产，是重要的生物柴油植物。基于小桐子种子发育过程中的EST序列，采用生物信息学方法，从4640个EST非冗余序列上鉴别了1009个SSR位点并分析其分布特征；开发了11对多态的EST-SSR分子标记，并利用这些分子标记调查了24个不同地理种源的遗传多样性，从每个位点的等位基因数目（2~3，平均为2.45）、期望杂合度（He为0.0887~0.5128，平均是0.2736）、多态信息含量（PIC为0.0847~0.4031，平均是0.2313）等方面反映了小桐子种质的遗传多样性低。进一步分析显示不同地理种源的遗传关系缺乏明显的地理结构。作者开发的EST-SSR分子标记不仅有助于小桐子种质的遗传多样性研究，也有助于小桐子种质间的遗传关系鉴别。

关键词: 小桐子, 生物柴油植物, EST-SSR, 遗传多样性

Abstract:

Jatropha curcas (Euphorbiaceae) has created tremendous interest all over the world for the use of its seed oil as a commercial source of biodiesel. Based on 9843 ESTs available from the developing seeds of Jatropha curcas, we identified 1009 SSRs in 4640 unigenes and developed 11 polymorphic EST-SSR markers which exhibited a low level of genetic diversity among germplasms, i.e. allele number varied from 2 to 3, with a average of 2.45; Heterozygosity (He) ranged from 0.0887-0.5128, with a average of 0.2736; Polymorphic Information Content (PIC) ranged from 0.0847-0.4031, with a average of 0.2313. Further, we analyzed the genetic relationships among 24 germplasms collected from different areas in southern China, northern Vietnam, and India using the 11 EST-SSR markers. The results showed that there was no a geographic pattern of genotypes across the collection areas of Jatropha curcas. The EST-SSR markers developed in current study is useful for both genetic diversity analysis and identification of genetic relationships among germplasms in Jatropha curcas.

Key words: Biodiesel plant, EST-SSR, Genetic diversity, Jatropha curcas

中图分类号:

Q 78

杨春1、2, 刘爱忠1. 小桐子EST-SSR分子标记的开发与种质遗传多样性分析[J]. Plant Diversity, 2011, 33(5): 529-534.

YANG Chun-, LIU Ai-Zhong. Development of EST-SSR Markers from Jatropha curcas (Euphorbiaceae) and Their Application in Genetic Diversity Analysis among Germplasms[J]. Plant Diversity, 2011, 33(5): 529-534.

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小桐子EST-SSR分子标记的开发与种质遗传多样性分析

Development of EST-SSR Markers from Jatropha curcas (Euphorbiaceae) and Their Application in Genetic Diversity Analysis among Germplasms

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