云南古茶园栽培大叶茶和大理茶群体的叶绿体RPL32-TRNL核苷酸变异和遗传分化

doi:10.3724/SP.J.1143.2010.10077

Plant Diversity ›› 2010, Vol. 32 ›› Issue (05): 427-434.DOI: 10.3724/SP.J.1143.2010.10077

云南古茶园栽培大叶茶和大理茶群体的叶绿体RPL32-TRNL核苷酸变异和遗传分化

刘阳1、2，杨世雄3，高立志1

1 中国科学院昆明植物研究所植物种质资源与基因组学研究中心，云南昆明650204；
2 中国科学院研究生院，北京100049；3 中国科学院昆明植物研究所
生物多样性与生物地理学重点实验室，云南昆明650204

收稿日期:2010-04-12 出版日期:2010-10-25 发布日期:2010-06-09
基金资助:
中国科学院昆明植物研究所“百人计划”引进人才启动项目(51O602511121)；昆明植物研究所创新三期领域前沿重点项目(672705232515)；云南省自然科学基金重点项目(2008CC016)；中国科学院“百人计划”择优支持项目；云南省高端科技人才引进项目(20080A009)；国家自然科学基金NSFC云南联合基金项目(U0936603)

Comparative Study on the Chloroplast RPL32-TRNL Nucleotide Variation within and Genetic Differentiation among Ancient
Tea Plantations of Camellia sinensis var.assamica and C.taliensis (Theaceae) from Yunnan, China

LIU Yang-1、2, YANG Shi-Xiong-3, GAO Li-Zhi-1

1 Plant Germplasm and Genomics Center, Kunming Institute of Botany, Chinese Academy of Sciences,
Kunming 650204, China; 2 Graduate University of Chinese Academy of Sciences, Beijing 100049, China；
3 Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany,
Chinese Academy of Sciences, Kunming 650204, China

Received:2010-04-12 Online:2010-10-25 Published:2010-06-09

摘要/Abstract

摘要：

对云南地区栽培的大叶茶(Camellia sinensis varassamica) 9个居群和大理茶(Ctaliensis) 3个居群的遗传多样性和居群遗传结构进行了比较研究。通过对94条叶绿体DNA RPL32TRNL序列的核苷酸序列变异进行分析，共发现了7个单倍型。研究结果表明，大叶茶(h=0728，π=000469)比大理茶(h=0610, π=000225)拥有更为丰富的遗传变异，但其居群遗传分化水平(Gst =0580, FST=0612)却低于大理茶(Gst=0696 , FST=0773)。AMOVA分析进一步证实了它们的遗传变异主要存在于居群间，且大叶茶(6121%)低于大理茶(7734%)。相对于大理茶，遗传多样性水平在本研究所取的大叶茶居群间存在着较大的差异，单倍型多态性水平变化范围为0~0809, 核苷酸多态性水平变化范围为0~000487。最后，讨论并提出了科学有效地保护我国古茶园茶种资源的建议和对策。

关键词: 大叶茶, 大理茶, 叶绿体DNA, 遗传多样性, 古茶园, RPL32-TRNL

Abstract:

In order to assess genetic diversity and population structure, nine Camellia sinensis var.assamica and three C.taliensis populations from Yunnan, China were evaluated. Based on nucleotide variation of 94 cpDNA RPL32-TRNL sequences, a total of seven distinct haplotypes were detected in the studied populations. It was apparent that C.sinensis var.assamica (h=0.728，π =0.00469) possessed higher levels of genetic diversity than C.taliensis (h =0.610, π =0.00225). However, genetic differentiation among populations of C.sinensis varassamica (Gst=0.580, FST=0.612) was lower than that among C.taliensis populations (Gst=0.696, FST=0.773). Our AMOVA analysis further revealed that the majority of variation was partitioned among populations of the two taxa, while C.sinensis var.assamica (6121%) showed lower level of genetic differentiation than C.taliensis (77.34%). In comparisons to C.taliensis, genetic diversity within C.sinensis var.assamica largely varied among populations, ranging from 0 to 0.809 and from 0 to 0.00487 for haplotype and nucleotide diversity, respectively. Conservation strategies were finally discussed and proposed to efficiently protect ancient tea plantations in China.

Key words: Camellia sinensis var.assamica, Camellia taliensis, cpDNA, Genetic diversity, Ancient tea plantations, RPL32-TRNL

中图分类号:

Q 16

刘阳1、2, 杨世雄3, 高立志1 . 云南古茶园栽培大叶茶和大理茶群体的叶绿体RPL32-TRNL核苷酸变异和遗传分化[J]. Plant Diversity, 2010, 32(05): 427-434.

LIU Yang-, YANG Shi-Xiong-, GAO Li-Zhi- . Comparative Study on the Chloroplast RPL32-TRNL Nucleotide Variation within and Genetic Differentiation among Ancient
Tea Plantations of Camellia sinensis var.assamica and C.taliensis (Theaceae) from Yunnan, China[J]. Plant Diversity, 2010, 32(05): 427-434.

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云南古茶园栽培大叶茶和大理茶群体的叶绿体RPL32-TRNL核苷酸变异和遗传分化

Comparative Study on the Chloroplast RPL32-TRNL Nucleotide Variation within and Genetic Differentiation among Ancient
Tea Plantations of Camellia sinensis var.assamica and C.taliensis (Theaceae) from Yunnan, China

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云南古茶园栽培大叶茶和大理茶群体的叶绿体RPL32-TRNL核苷酸变异和遗传分化

Comparative Study on the Chloroplast RPL32-TRNL Nucleotide Variation within and Genetic Differentiation among Ancient Tea Plantations of Camellia sinensis var.assamica and C.taliensis (Theaceae) from Yunnan, China

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Comparative Study on the Chloroplast RPL32-TRNL Nucleotide Variation within and Genetic Differentiation among Ancient
Tea Plantations of Camellia sinensis var.assamica and C.taliensis (Theaceae) from Yunnan, China