Identification and Characterization of a d-Cadinol Synthase Potentially Involved in the Formation of Boreovibrins in Boreostereum vibrans of Basidiomycota

doi:10.1007/s13659-016-0096-4

Natural Products and Bioprospecting ›› 2016, Vol. 6 ›› Issue (3): 167-171.DOI: 10.1007/s13659-016-0096-4

• Original article • Previous Articles Next Articles

Identification and Characterization of a d-Cadinol Synthase Potentially Involved in the Formation of Boreovibrins in Boreostereum vibrans of Basidiomycota

Hui Zhou^1,2, Yan-Long Yang^1,2, Jun Zeng^1,2, Ling Zhang¹, Zhi-Hui Ding¹, Ying Zeng¹

1. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, 132 Lanhei Road, Kunming 650201, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China

Received:2016-03-03 Revised:2016-03-24 Online:2018-02-08 Published:2016-06-24
Supported by:
This work was supported by a grant from the National Natural Science Foundation of China (21572237);We are grateful to Professor Jian-Xin Pu (Kunming Institute of Botany) for his helpful suggestion

Identification and Characterization of a d-Cadinol Synthase Potentially Involved in the Formation of Boreovibrins in Boreostereum vibrans of Basidiomycota

Hui Zhou^1,2, Yan-Long Yang^1,2, Jun Zeng^1,2, Ling Zhang¹, Zhi-Hui Ding¹, Ying Zeng¹

1. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, 132 Lanhei Road, Kunming 650201, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China

通讯作者: Ying Zeng,e-mail:biochem@mail.kib.ac.cn
基金资助:
This work was supported by a grant from the National Natural Science Foundation of China (21572237);We are grateful to Professor Jian-Xin Pu (Kunming Institute of Botany) for his helpful suggestion

Abstract

Abstract: Sesquiterpenoids are very common among natural products. A large number of sesquiterpene synthase genes have been cloned and functionally characterized. However, until now there is no report about the δ-cadinol synthase predominantly forming d-cadinol (syn. torreyol) from farnesyl diphosphate. Sesquiterpenoids boreovibrins structurally similar to δ-cadinol were previously isolated from culture broths of the basidiomycete fungus Boreostereum vibrans. This led us to expect a corresponding gene coding for a δ-cadinol synthase that may be involved in the biosynthesis of boreovibrins in B. vibrans. Here we report the cloning and heterologous expression of a new sesquiterpene synthase gene from B. vibrans. The crude and purified recombinant enzymes, when incubating with farnesyl diphosphate as substrate, gave δ-cadinol as its principal product and thereby identified as a δ-cadinol synthase.

Key words: Delta-cadinol, Sesquiterpene synthase, Biosynthesis, GC-MS, Fungi

摘要： Sesquiterpenoids are very common among natural products. A large number of sesquiterpene synthase genes have been cloned and functionally characterized. However, until now there is no report about the δ-cadinol synthase predominantly forming d-cadinol (syn. torreyol) from farnesyl diphosphate. Sesquiterpenoids boreovibrins structurally similar to δ-cadinol were previously isolated from culture broths of the basidiomycete fungus Boreostereum vibrans. This led us to expect a corresponding gene coding for a δ-cadinol synthase that may be involved in the biosynthesis of boreovibrins in B. vibrans. Here we report the cloning and heterologous expression of a new sesquiterpene synthase gene from B. vibrans. The crude and purified recombinant enzymes, when incubating with farnesyl diphosphate as substrate, gave δ-cadinol as its principal product and thereby identified as a δ-cadinol synthase.

关键词: Delta-cadinol, Sesquiterpene synthase, Biosynthesis, GC-MS, Fungi

Hui Zhou, Yan-Long Yang, Jun Zeng, Ling Zhang, Zhi-Hui Ding, Ying Zeng. Identification and Characterization of a d-Cadinol Synthase Potentially Involved in the Formation of Boreovibrins in Boreostereum vibrans of Basidiomycota[J]. Natural Products and Bioprospecting, 2016, 6(3): 167-171.

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Identification and Characterization of a d-Cadinol Synthase Potentially Involved in the Formation of Boreovibrins in Boreostereum vibrans of Basidiomycota

Identification and Characterization of a d-Cadinol Synthase Potentially Involved in the Formation of Boreovibrins in Boreostereum vibrans of Basidiomycota

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