Characterization of Chilean hot spring-origin Staphylococcus sp. BSP3 produced exopolysaccharide as biological additive

doi:10.1007/s13659-024-00436-0

Natural Products and Bioprospecting ›› 2024, Vol. 14 ›› Issue (2): 3-3.DOI: 10.1007/s13659-024-00436-0

• ORIGINAL ARTICLES • Previous Articles Next Articles

Characterization of Chilean hot spring-origin Staphylococcus sp. BSP3 produced exopolysaccharide as biological additive

Srijan Banerjee¹, Gustavo Cabrera-Barjas², Jaime Tapia¹, João Paulo Fabi^3,4, Cedric Delattre^5,6, Aparna Banerjee⁷

1. Instituto de Química de Recursos Naturales, Universidad de Talca, CP 3460000, Talca, Chile;
2. Universidad San Sebastián Campus Las Tres Pascualas, Facultad de Ciencias Para el Cuidado de la Salud, Lientur 1457, CP 4080871, Concepción, Chile;
3. Department of Food Science and Experimental Nutrition, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP, Brazil;
4. Food Research Center (FoRC), CePID-FAPESP (Research, Innovation and Dissemination Centers, São Paulo Research Foundation), São Paulo, SP, Brazil;
5. Université Clermont Auvergne, Clermont Auvergne INP, CNRS, Institut Pascal, 63000, Clermont-Ferrand, France;
6. Institut Universitaire de France (IUF), 1 Rue Descartes, 75005, Paris, France;
7. Instituto de Ciencias Aplicadas, Facultad de Ingeniería, Universidad Autónoma de Chile, CP 3467987, Talca, Chile

Received:2023-12-07 Online:2024-05-16 Published:2024-04-24
Contact: Aparna Banerjee,E-mail:aparna.banerjee@uautonoma.cl
Supported by:
This research was funded by FONDECYT Regular, Grant Number 1231917 by ANID, Govt. of Chile.

Characterization of Chilean hot spring-origin Staphylococcus sp. BSP3 produced exopolysaccharide as biological additive

Srijan Banerjee¹, Gustavo Cabrera-Barjas², Jaime Tapia¹, João Paulo Fabi^3,4, Cedric Delattre^5,6, Aparna Banerjee⁷

1. Instituto de Química de Recursos Naturales, Universidad de Talca, CP 3460000, Talca, Chile;
2. Universidad San Sebastián Campus Las Tres Pascualas, Facultad de Ciencias Para el Cuidado de la Salud, Lientur 1457, CP 4080871, Concepción, Chile;
3. Department of Food Science and Experimental Nutrition, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP, Brazil;
4. Food Research Center (FoRC), CePID-FAPESP (Research, Innovation and Dissemination Centers, São Paulo Research Foundation), São Paulo, SP, Brazil;
5. Université Clermont Auvergne, Clermont Auvergne INP, CNRS, Institut Pascal, 63000, Clermont-Ferrand, France;
6. Institut Universitaire de France (IUF), 1 Rue Descartes, 75005, Paris, France;
7. Instituto de Ciencias Aplicadas, Facultad de Ingeniería, Universidad Autónoma de Chile, CP 3467987, Talca, Chile

通讯作者: Aparna Banerjee,E-mail:aparna.banerjee@uautonoma.cl
基金资助:
This research was funded by FONDECYT Regular, Grant Number 1231917 by ANID, Govt. of Chile.

Abstract

Abstract: A type of high molecular weight bioactive polymers called exopolysaccharides (EPS) are produced by thermophiles, the extremophilic microbes that thrive in acidic environmental conditions of hot springs with excessively warm temperatures. Over time, EPS became important as natural biotechnological additives because of their noncytotoxic, emulsifying, antioxidant, or immunostimulant activities. In this article, we unravelled a new EPS produced by Staphylococcus sp. BSP3 from an acidic (pH 6.03) San Pedro hot spring (38.1 ℃) located in the central Andean mountains in Chile. Several physicochemical techniques were performed to characterize the EPS structure including Scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDS), Atomic Force Microscopy (AFM), High-Performance Liquid Chromatography (HPLC), Gel permeation chromatography (GPC), Fourier Transform Infrared Spectroscopy (FTIR), 1D Nuclear Magnetic Resonance (NMR), and Thermogravimetric analysis (TGA). It was confirmed that the amorphous surface of the BSP3 EPS, composed of rough pillar-like nanostructures, is evenly distributed. The main EPS monosaccharide constituents were mannose (72%), glucose (24%) and galactose (4%). Also, it is a medium molecular weight (43.7 kDa) heteropolysaccharide. NMR spectroscopy demonstrated the presence of a [→6)-α-d-Manp-(1→6)-α-d-Manp-(1→] backbone 2-O substituted with 1-α-d-Manp. A high thermal stability of EPS (287 ℃) was confirmed by TGA analysis. Emulsification, antioxidant, flocculation, water-holding (WHC), and oil-holding (OHC) capacities are also studied for biotechnological industry applications. The results demonstrated that BSP3 EPS could be used as a biodegradable material for different purposes, like flocculation and natural additives in product formulation.

Key words: Staphylococcus, Hot spring, Exopolysaccharides, Structural characterization, Flocculation, Antioxidant activity

摘要： A type of high molecular weight bioactive polymers called exopolysaccharides (EPS) are produced by thermophiles, the extremophilic microbes that thrive in acidic environmental conditions of hot springs with excessively warm temperatures. Over time, EPS became important as natural biotechnological additives because of their noncytotoxic, emulsifying, antioxidant, or immunostimulant activities. In this article, we unravelled a new EPS produced by Staphylococcus sp. BSP3 from an acidic (pH 6.03) San Pedro hot spring (38.1 ℃) located in the central Andean mountains in Chile. Several physicochemical techniques were performed to characterize the EPS structure including Scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDS), Atomic Force Microscopy (AFM), High-Performance Liquid Chromatography (HPLC), Gel permeation chromatography (GPC), Fourier Transform Infrared Spectroscopy (FTIR), 1D Nuclear Magnetic Resonance (NMR), and Thermogravimetric analysis (TGA). It was confirmed that the amorphous surface of the BSP3 EPS, composed of rough pillar-like nanostructures, is evenly distributed. The main EPS monosaccharide constituents were mannose (72%), glucose (24%) and galactose (4%). Also, it is a medium molecular weight (43.7 kDa) heteropolysaccharide. NMR spectroscopy demonstrated the presence of a [→6)-α-d-Manp-(1→6)-α-d-Manp-(1→] backbone 2-O substituted with 1-α-d-Manp. A high thermal stability of EPS (287 ℃) was confirmed by TGA analysis. Emulsification, antioxidant, flocculation, water-holding (WHC), and oil-holding (OHC) capacities are also studied for biotechnological industry applications. The results demonstrated that BSP3 EPS could be used as a biodegradable material for different purposes, like flocculation and natural additives in product formulation.

关键词: Staphylococcus, Hot spring, Exopolysaccharides, Structural characterization, Flocculation, Antioxidant activity

Srijan Banerjee, Gustavo Cabrera-Barjas, Jaime Tapia, João Paulo Fabi, Cedric Delattre, Aparna Banerjee. Characterization of Chilean hot spring-origin Staphylococcus sp. BSP3 produced exopolysaccharide as biological additive[J]. Natural Products and Bioprospecting, 2024, 14(2): 3-3.

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Characterization of Chilean hot spring-origin Staphylococcus sp. BSP3 produced exopolysaccharide as biological additive

Characterization of Chilean hot spring-origin Staphylococcus sp. BSP3 produced exopolysaccharide as biological additive

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