Preparation, physicochemical properties, and immunomodulatory activity of glycoproteins from Syngnathoides biaculeatus

doi:10.1007/s13659-025-00551-6

Natural Products and Bioprospecting ›› 2025, Vol. 15 ›› Issue (6): 66-66.DOI: 10.1007/s13659-025-00551-6

• Original Article • Previous Articles Next Articles

Preparation, physicochemical properties, and immunomodulatory activity of glycoproteins from Syngnathoides biaculeatus

Xuewei Xia¹, Jieling Lin^1,2, Lihong Yang^1,3, Youhong Li³, Wenshen Lin³, Qingqing Wang¹, Jun Liu⁴, Riming Huang¹

1. Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou, 510642, China;
2. Guangdong Eco-Engineering Polytechnic, Guangzhou, 510520, China;
3. Guangzhou University of Chinese Medicine, Guangzhou, 510006, China;
4. Laboratory of Pathogenic Biology, Guangdong Medical University, Zhanjiang, 524023, China

Received:2025-06-01 Online:2026-01-12
Contact: Jun Liu Email:E-mail:lj2388240@gdmu.edu.cn
Supported by:
This work was financially supported by the Key-Area Research and Development Program of Guangdong Province (No. 2022B0202020004), Medical Scientific Research Foundation of Guangdong Province (No. A2025237), Shenzhen Science and Technology Program (No. KCXFZ20240903094014019), Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety (No. GDPKLAPPS2304 and GDPKLAPPS2102).

Preparation, physicochemical properties, and immunomodulatory activity of glycoproteins from Syngnathoides biaculeatus

Xuewei Xia¹, Jieling Lin^1,2, Lihong Yang^1,3, Youhong Li³, Wenshen Lin³, Qingqing Wang¹, Jun Liu⁴, Riming Huang¹

1. Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou, 510642, China;
2. Guangdong Eco-Engineering Polytechnic, Guangzhou, 510520, China;
3. Guangzhou University of Chinese Medicine, Guangzhou, 510006, China;
4. Laboratory of Pathogenic Biology, Guangdong Medical University, Zhanjiang, 524023, China

通讯作者: Jun Liu Email:E-mail:lj2388240@gdmu.edu.cn
作者简介:Riming Huang Email:E-mail:huangriming@scau.edu.cn
基金资助:
This work was financially supported by the Key-Area Research and Development Program of Guangdong Province (No. 2022B0202020004), Medical Scientific Research Foundation of Guangdong Province (No. A2025237), Shenzhen Science and Technology Program (No. KCXFZ20240903094014019), Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety (No. GDPKLAPPS2304 and GDPKLAPPS2102).

Abstract

Abstract: Syngnathoides biaculeatus, a potential functional food from marine sources, was found to enhance nonspecific immunity, but its functional ingredients have rarely been reported. Therefore, this study focused on the preparation and physicochemical properties of its water-soluble natural ingredients with their immunomodulatory activities and potential mechanisms. Firstly, we optimized the extraction method of glycoproteins from S. biaculeatus and prepared the crude glycoprotein SYB, from which the fraction glycoprotein SYB-1 was further purified. The carbohydrates of SYB-1 were 8.46% comprising mannose, glucose, and galactose, with an average molecular weight of 9.423 kDa. Amino acid analysis demonstrated that its major amino acids are glycine, glutamic acid, aspartic acid, and proline, with a total amino acid content of 88.81%. Furthermore, SYB-1 could significantly increase the cell viability of macrophages, and promote the release of NO, TNF-α, and IL-6. Metabolomics revealed that it was associated with arachidonic acid metabolism. The CYP450 enzyme family members and PTGS2 may be key targets for the regulatory role. These results suggested that the glycoprotein of S. biaculeatus may be an attractive functional food supplement from natural sources for immunocompromised populations.

Key words: Syngnathoides biaculeatus, Glycoprotein, Physicochemical properties, Immunomodulatory activity

摘要： Syngnathoides biaculeatus, a potential functional food from marine sources, was found to enhance nonspecific immunity, but its functional ingredients have rarely been reported. Therefore, this study focused on the preparation and physicochemical properties of its water-soluble natural ingredients with their immunomodulatory activities and potential mechanisms. Firstly, we optimized the extraction method of glycoproteins from S. biaculeatus and prepared the crude glycoprotein SYB, from which the fraction glycoprotein SYB-1 was further purified. The carbohydrates of SYB-1 were 8.46% comprising mannose, glucose, and galactose, with an average molecular weight of 9.423 kDa. Amino acid analysis demonstrated that its major amino acids are glycine, glutamic acid, aspartic acid, and proline, with a total amino acid content of 88.81%. Furthermore, SYB-1 could significantly increase the cell viability of macrophages, and promote the release of NO, TNF-α, and IL-6. Metabolomics revealed that it was associated with arachidonic acid metabolism. The CYP450 enzyme family members and PTGS2 may be key targets for the regulatory role. These results suggested that the glycoprotein of S. biaculeatus may be an attractive functional food supplement from natural sources for immunocompromised populations.

关键词: Syngnathoides biaculeatus, Glycoprotein, Physicochemical properties, Immunomodulatory activity

Xuewei Xia, Jieling Lin, Lihong Yang, Youhong Li, Wenshen Lin, Qingqing Wang, Jun Liu, Riming Huang. Preparation, physicochemical properties, and immunomodulatory activity of glycoproteins from Syngnathoides biaculeatus[J]. Natural Products and Bioprospecting, 2025, 15(6): 66-66.

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Preparation, physicochemical properties, and immunomodulatory activity of glycoproteins from Syngnathoides biaculeatus

Preparation, physicochemical properties, and immunomodulatory activity of glycoproteins from Syngnathoides biaculeatus

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