Novel peptaibiotics identified from Trichoderma clade Viride

doi:10.1007/s13659-025-00524-9

Natural Products and Bioprospecting ›› 2025, Vol. 15 ›› Issue (5): 48-48.DOI: 10.1007/s13659-025-00524-9

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Novel peptaibiotics identified from Trichoderma clade Viride

Tamás Marik¹, Bonaya Gufu¹, Anusha Vishwanathula¹, Dóra Balázs^1,2, Ákos Rozsnyói^1,2, Gergő Terna^1,2, Fanni Kovács^1,2, Sándor Kocsubé^1,3, Mónika Varga¹, András Szekeres¹, Irina S. Druzhinina⁴, Csaba Vágvölgyi^1,3, Tamás Papp^1,3, Chetna Tyagi¹, László Kredics¹

1. Department of Biotechnology and Microbiology, Faculty of Science and Informatics, University of Szeged, Közép Fasor 52, 6726, Szeged, Hungary;
2. Doctoral School in Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary;
3. HUN-REN-SZTE Fungal Pathomechanisms Research Group, University of Szeged, Szeged, Hungary;
4. Royal Botanic Gardens, Kew, Kew Green, Richmond, Surrey, TW9 3AE, UK

Received:2025-03-18 Online:2025-11-06
Contact: Tamás Marik,E-mail:marik.tamas@szte.hu;László Kredics,E-mail:kredics@bio.u-szeged.hu
Supported by:
T.M. and D. B. was supported by the Sholarship Program of the Ministry of Culture and Innovation, Financed from the National Research, Development and Innovation Fund (EKÖP-24-4SZTE-629, EKÖP-24-4-SZTE-605, respectively). C.T. is supported by the short-term KGYNK-2024 grant from the Hungarian Academy of Sciences (HAS). S.K., C.V. and T.P. were supported by the grants HUN-REN 2001007 and TKP2021-EGA-28.

Novel peptaibiotics identified from Trichoderma clade Viride

1. Department of Biotechnology and Microbiology, Faculty of Science and Informatics, University of Szeged, Közép Fasor 52, 6726, Szeged, Hungary;
2. Doctoral School in Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary;
3. HUN-REN-SZTE Fungal Pathomechanisms Research Group, University of Szeged, Szeged, Hungary;
4. Royal Botanic Gardens, Kew, Kew Green, Richmond, Surrey, TW9 3AE, UK

通讯作者: Tamás Marik,E-mail:marik.tamas@szte.hu;László Kredics,E-mail:kredics@bio.u-szeged.hu
基金资助:
T.M. and D. B. was supported by the Sholarship Program of the Ministry of Culture and Innovation, Financed from the National Research, Development and Innovation Fund (EKÖP-24-4SZTE-629, EKÖP-24-4-SZTE-605, respectively). C.T. is supported by the short-term KGYNK-2024 grant from the Hungarian Academy of Sciences (HAS). S.K., C.V. and T.P. were supported by the grants HUN-REN 2001007 and TKP2021-EGA-28.

Abstract

Abstract: This study focuses on the peptaibiome produced by different species of Trichoderma belonging to clade Viride: T. koningii SZMC 28387 (CBS 979.70), T. cf. strigosellum SZMC 28007 (TUCIM 4886/IQ 191), T. cf. dorothopsis SZMC 28390 (TUCIM 416/TUB F-597), T. cf. strigosellum SZMC 28391 (TUCIM 423/DAOM 230018), T. atroviride SZMC 28748 (IMI 206040), T. hamatum SZMC 28747 (TUCIM 2730) and T. cf. dorothopsis SZMC 28005 (TUCIM 4882/IQ 11). We were able to identify new compounds with similarity to already known groups of peptaibiotics, as well as completely newly discovered compounds using high-performance liquid chromatography (HPLC) -mass spectrometry (MS). From the 367 peptaibiotics identified, 216 are peptaibols and 111 are lipopeptaibols. Out of all peptaibols, 55 are previously known, while 161 are newly discovered. The new peptaibol subgroups Strigosellin A, B and Dorothopsin A, B are introduced. Furthermore, besides 38 previously known lipopeptaibols, 73 new lipopeptaibol sequences, named Lipostrigosellins and Lipohamatins are also reported. In addition, 41 peptaibol-like compounds with unusual C-terminus were also found. Out of the 7 strains examined, 5 produced both peptaibols and lipopeptaibols, while 2 only peptaibols. The well-known compound, Trikoningin KA V (TRK-V) also produced by T. koningii SZMC 28387 (CBS 979.70), was studied for its folding dynamics using accelerated molecular dynamics simulations (aMD) for understanding the plausible three-dimensional structures adopted by these peptaibols of clade Viride. We observed a propensity to form kinked, right-handed helical structures when simulated in an aqueous environment.

Key words: Trichoderma, Clade Viride, Peptaibol, Lipopeptaibol, Peptaibiome, HPLC–MS, Accelerated MD simulations

摘要： This study focuses on the peptaibiome produced by different species of Trichoderma belonging to clade Viride: T. koningii SZMC 28387 (CBS 979.70), T. cf. strigosellum SZMC 28007 (TUCIM 4886/IQ 191), T. cf. dorothopsis SZMC 28390 (TUCIM 416/TUB F-597), T. cf. strigosellum SZMC 28391 (TUCIM 423/DAOM 230018), T. atroviride SZMC 28748 (IMI 206040), T. hamatum SZMC 28747 (TUCIM 2730) and T. cf. dorothopsis SZMC 28005 (TUCIM 4882/IQ 11). We were able to identify new compounds with similarity to already known groups of peptaibiotics, as well as completely newly discovered compounds using high-performance liquid chromatography (HPLC) -mass spectrometry (MS). From the 367 peptaibiotics identified, 216 are peptaibols and 111 are lipopeptaibols. Out of all peptaibols, 55 are previously known, while 161 are newly discovered. The new peptaibol subgroups Strigosellin A, B and Dorothopsin A, B are introduced. Furthermore, besides 38 previously known lipopeptaibols, 73 new lipopeptaibol sequences, named Lipostrigosellins and Lipohamatins are also reported. In addition, 41 peptaibol-like compounds with unusual C-terminus were also found. Out of the 7 strains examined, 5 produced both peptaibols and lipopeptaibols, while 2 only peptaibols. The well-known compound, Trikoningin KA V (TRK-V) also produced by T. koningii SZMC 28387 (CBS 979.70), was studied for its folding dynamics using accelerated molecular dynamics simulations (aMD) for understanding the plausible three-dimensional structures adopted by these peptaibols of clade Viride. We observed a propensity to form kinked, right-handed helical structures when simulated in an aqueous environment.

关键词: Trichoderma, Clade Viride, Peptaibol, Lipopeptaibol, Peptaibiome, HPLC–MS, Accelerated MD simulations

Tamás Marik, Bonaya Gufu, Anusha Vishwanathula, Dóra Balázs, Ákos Rozsnyói, Gergő Terna, Fanni Kovács, Sándor Kocsubé, Mónika Varga, András Szekeres, Irina S. Druzhinina, Csaba Vágvölgyi, Tamás Papp, Chetna Tyagi, László Kredics. Novel peptaibiotics identified from Trichoderma clade Viride[J]. Natural Products and Bioprospecting, 2025, 15(5): 48-48.

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Novel peptaibiotics identified from Trichoderma clade Viride

Novel peptaibiotics identified from Trichoderma clade Viride

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