Morphological Effects of Natural Products on Schizosaccharomyces pombe Measured by Imaging Flow Cytometry

doi:10.1007/s13659-014-0004-8

Abstract

Abstract: Gaining a full understanding of the mechanisms of action of natural products as therapeutic agents includes observing the effects of natural products on cellular morphology, because abnormal cellular morphology is an important aspect of cellular transformations that occur as part of disease states. In this study a set of natural products was examined in search of small molecules that influence the cylindrical morphology of fission yeast Schizosaccharomyces pombe. Imaging flow cytometry of large populations of S. pombe exposed to natural products captured cell images and revealed changes in mean length and aspect ratio of cells. Several natural products were found to alter S. pombe's morphology relative to control, in terms of elongating cells, shrinking them, or making them more round. These results may facilitate future investigations into methods by which cells establish and maintain specific shapes.

Key words: Schizosaccharomyces pombe, Morphology, Natural products, Imaging flow cytometry, Aspect ratio, Fission yeast

摘要： Gaining a full understanding of the mechanisms of action of natural products as therapeutic agents includes observing the effects of natural products on cellular morphology, because abnormal cellular morphology is an important aspect of cellular transformations that occur as part of disease states. In this study a set of natural products was examined in search of small molecules that influence the cylindrical morphology of fission yeast Schizosaccharomyces pombe. Imaging flow cytometry of large populations of S. pombe exposed to natural products captured cell images and revealed changes in mean length and aspect ratio of cells. Several natural products were found to alter S. pombe's morphology relative to control, in terms of elongating cells, shrinking them, or making them more round. These results may facilitate future investigations into methods by which cells establish and maintain specific shapes.

关键词: Schizosaccharomyces pombe, Morphology, Natural products, Imaging flow cytometry, Aspect ratio, Fission yeast

Joel Heisler, Lindsay Elvir, Farah Barnouti, Erica Charles, Tom D. Wolkow, Radha Pyati. Morphological Effects of Natural Products on Schizosaccharomyces pombe Measured by Imaging Flow Cytometry[J]. Natural Products and Bioprospecting, 2014, 4(1): 27-35.

References

1. D. Hanahan, R.A. Weinberg, Cell 100, 57-70 (2000)
2. L. Yin, W.J. Cai, C.X. Liu, Y.Z. Chen, J.M. Hu, J.F. Jiang, H.A. Li, X.B. Cui, X.Y. Chang, W.J. Zhang, K. Sun, F. Li, PLoS One 8, 62971 (2013)
3. S. Tanaka, K. Nakamura, N. Takahasi, T. Suda, Immunol. Rev. 208, 30-49 (2005)
4. V. Starkuviene, R. Pepperkok, Br. J. Pharmacol. 152, 62-71 (2007)
5. J. Hayles, P. Nurse, Nat. Rev. Mol. Cell Biol. 2, 647-656 (2001)
6. T.G. Hart, A.K. Ramani, E.M. Marcotte, Genome Biol. 7, 1201-1209 (2006)
7. R. Pyati, L.L. Elvir, E.C. Charles, U. Seenath, T.D.J. Wolkow, Yeast Fungal Res. 2, 106-112 (2011)
8. J.C. Ford, F. al-Khodairy, E. Fotou, K.S. Sheldrick, D.J. Griffiths, A.M. Carr, Science 265, 533-535 (1994)
9. NCI Yeast Anticancer Drug Screen Online Database (2002), http://dtp.nci.nih.gov/yacds/nscsearch.html. Accessed 27 Nov 2013
10. R.G. Mortimer, Mathematics for Physical Chemistry (Macmillan Publishing, New York, 1981)
11. R. Vrzal, K. Frauenstein, P. Proksch, J. Abel, Z. Dvorak, T. Haarmann-Stemmann, PLoS One 8, e74917 (2013)
12. W. Martindale, The Extra Pharmacopeia, 30th edn. (Pharmaceutical Press, London, 2011), p. 124
13. J.K. Lee, J.S. Jung, S.H. Park, S.H. Park, Y.B. Sim, S.M. Kim, T.S. Ha, H.W. Suh, Arch. Pharm. Res. 33, 1843-1850 (2010)
14. J.C.G. Cortes, R. Katoh-Fukui, K. Moto, J.C. Ribas, J. Ishiguro, Eukaryot. Cell 3, 1124-1135 (2004)
15. S.R. Meshnick, Int. J. Parasitol. 32, 1655-1660 (2002)
16. T. Kondo, D. FitzGerald, V.K. Chaudhary, S. Adhya, I.J. Pastan, Biol. Chem. 263, 9470-9475 (1988)
17. J.R. Chandler, T.T. Truong, P.M. Silva, M.R. Seyedsayamdost, G. Carr, M. Radey, M.A. Jacobs, E.H. Sims, J. Clardy, E.P. Greenberg, mBio 3, e00499-e00512 (2012)
18. M. Kawada, M. Amemiya, M. Ishizuka, T. Takeuchi, Jpn. J. Cancer Res. 90, 219-225 (1999)
19. B. Alberts, A. Johnson, J. Lewis, M. Raff, K. Roberts, P. Walter, Molecular Biology of the Cell, 4th edn. (Garland Science, New York, 2002)
20. D. Carmona-Gutierrez, T. Eisenberg, S. Buttner, C. Meisinger, G. Kroemer, F. Madeo, Cell Death Differ. 17, 763-773 (2010)
21. M. Morimoto, M. Fukui, S. Ohkubo, T. Tamaoki, F. Tomita, J. Antibiot. 35, 1033-1037 (1982)
22. T. Nakashima, M. Miura, M. Hara, Cancer Res. 60, 1229-1235 (2000)
23. J.M. Jürgensmeier, S. Krajewski, R.C. Armstrong, G.M. Wilson, T. Oltersdorf, L.C. Fritz, J.C. Reed, S. Ottilie, Mol. Biol. Cell 8, 325-339 (1997)
24. E.C. Higbee, Econ. Bot. 1, 427-436 (1947)
25. N.V.S. Rao, T.R.P. Seshadri, Indian Acad. Sci. A 24, 365-374 (1946)
26. A. Prakash, J. Rao, Botanical Pesticides in Agriculture (CRC Press, Boca Raton, 1997), p. 163
27. J.B. McMahon, M.J. Currens, R.J. Gulakowski, R.W. Buckheit Jr, C. Lackman-Smith, Y.F. Hallock, M.R. Boyd, Antimicrob. Agents Chemother. 39, 484-488 (1995)
28. G. Xu, W. Fu, G. Liu, C.H. Senanayake, W. Tang, J. Am. Chem. Soc. 136(2), 570-573 (2014)
29. E.L. White, W. Chao, L.J. Ross, D.W. Borhani, P.D. Hobbs, V. Upender, M.I. Dawson, Arch. Biochem. Biophys. 365, 25-30 (1999)
30. M. Arellano, M.H. Valdivieso, T.M. Calonge, P.M. Coll, A. Duran, P. Perez, J. Cell Sci. 112, 3569-3578 (1999)
31. B. Santos, J. Gutierrez, T.M. Calonge, P. Perez, Eukaryot. Cell 2, 521-533 (2003)
32. A. Ghantous, A. Sinjab, Z. Herceg, N. Darwiche, Drug Discov. Today 18, 894-905 (2013)
33. A.D. Aires, E.C. Ximenes, V.X. Barbosa, A.J. Góes, V.M. Souza, M.C. Albuquerque, Phytomedicine (2013). doi:10.1016/j. phymed.2013.08.012
34. B.D. Walker, M. Kowalski, W.C. Goh, K. Kozarsky, M. Krieger, C. Rosen, L. Rohrschneider, W.A. Haseltine, J. Sodroski, Proc. Natl. Acad. Sci. USA 84, 8120-8124 (1987)
35. J. Chang, T.M. Block, J.T. Guo, Antivir. Res. 99, 251-260 (2013)
36. E. Duenas-Santero, A.B. Martin-Cuadrado, T. Fontaine, J. Latge, F. Rey, C.V. Aldana, Eukaryot. Cell 9, 1650-1660 (2010)
37. C. Owa, M.E. Messina Jr, R. Halaby, Int. J. Womens Health 5, 557-569 (2013)
38. T. Iwaki, A. Hosomi, S. Tokudomi, Y. Kusunoki, Y. Fujita, Y. Giga-Hama, N. Tanaka, K. Takegawa, Microbiology 152, 1523-1532 (2006)
39. F. Van Assendelft, J.W. Miller, D.T. Mintz, J.A. Schack, P. Ottolenghi, H. Most, Am. J. Trop. Med. Hyg. 5, 501-503 (1956)
40. I.H. Hall, K.H. Lee, Y. Imakura, M. Okano, A. Johnson, J. Pharm. Sci. 72, 1282-1284 (2006)
41. M. Laundry, J. Elias, C. Pessoa, M. Odorico, L.V. Costa-Lotufo, R. Grougnet, S. Michel, F. Tillequin, L.S. Espindola, J. Ethnopharmacol. 123, 439-445 (2009)
42. I.H. Hall, Y.F. Liou, K.H. Lee, S.G. Chaney, W. Willingham Jr, J. Pharm. Sci. 72, 626-630 (2006)
43. J. Trujillo, Y.I. Chirino, C. Molina-Jijon, A.C. Anderica-Romero, E. Tapia, J. Pedraza-Chaverri, Redox Biol. 1, 448-456 (2013)
44. Y.R. Chen, T.H. Tan, Oncogene 17, 173-178 (1998)
45. S. Moreno, A. Klar, P. Nurse, Methods Enzymol. 194, 795-823 (1991)

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