Study of the distribution profile of piperidine alkaloids in various parts of Prosopis juliflora by the application of Direct Analysis in Real Time Mass Spectrometry (DART-MS)

doi:10.1007/s13659-012-0069-1

Natural Products and Bioprospecting ›› 2012, Vol. 2 ›› Issue (5): 206-209.DOI: 10.1007/s13659-012-0069-1

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Study of the distribution profile of piperidine alkaloids in various parts of Prosopis juliflora by the application of Direct Analysis in Real Time Mass Spectrometry (DART-MS)

Shachi SINGH, Sanjay Kumar VERMA

Center for Biotechnology, Department of Biological Sciences, Birla Institute of Technology and Science, Pilani 333031, India

Received:2012-08-15 Revised:2012-10-07 Online:2018-02-11 Published:2012-10-24
Supported by:
We acknowledge Birla Institute of Technology and Science for providing laboratory facilities and Central Drug Research Institute, Lucknow, for performing DART and LC-MS/MS analysis. Shachi Singh acknowledges the CSIR for fund support in the form of Senior Research Fellowship.

Study of the distribution profile of piperidine alkaloids in various parts of Prosopis juliflora by the application of Direct Analysis in Real Time Mass Spectrometry (DART-MS)

Shachi SINGH, Sanjay Kumar VERMA

Center for Biotechnology, Department of Biological Sciences, Birla Institute of Technology and Science, Pilani 333031, India

通讯作者: Shachi SINGH,E-mail:singhshachi@gmail.com
基金资助:
We acknowledge Birla Institute of Technology and Science for providing laboratory facilities and Central Drug Research Institute, Lucknow, for performing DART and LC-MS/MS analysis. Shachi Singh acknowledges the CSIR for fund support in the form of Senior Research Fellowship.

Abstract

Abstract: Direct Analysis in Real Time Mass Spectrometry(DART-MS) was applied to identify and study the distribution profile of piperidine alkaloids in different parts of Prosopis juliflora, without isolation and separation of the compounds by standard chromatographic techniques. With the help of DART-MS, chemical fingerprint of raw plant parts were generated, which revealed the presence of piperidine alkaloids in leaf, pod and flower. A comparative study of the distribution pattern, showed variation in the presence and distribution of these alkaloids in various parts of P. juliflora. The leaves and pod displayed the largest alkaloid pattern with a total of 12 different alkaloids in each part, whereas only 4 alkaloids were present in flower. Alkaloids:julifloridine, prosopine, prosopinine and prosafrinine were ubiquitously distributed in all the alkaloid rich plant parts. Juliprosopine was pre-eminet alkaloid in leaf, whereas pod and flower displayed copious amounts of julifloridine.

Key words: DART-MS, chemical fingerprint, Prosopis juliflora, piperidine alkaloids, juliprosopine, julifloridine

摘要： Direct Analysis in Real Time Mass Spectrometry(DART-MS) was applied to identify and study the distribution profile of piperidine alkaloids in different parts of Prosopis juliflora, without isolation and separation of the compounds by standard chromatographic techniques. With the help of DART-MS, chemical fingerprint of raw plant parts were generated, which revealed the presence of piperidine alkaloids in leaf, pod and flower. A comparative study of the distribution pattern, showed variation in the presence and distribution of these alkaloids in various parts of P. juliflora. The leaves and pod displayed the largest alkaloid pattern with a total of 12 different alkaloids in each part, whereas only 4 alkaloids were present in flower. Alkaloids:julifloridine, prosopine, prosopinine and prosafrinine were ubiquitously distributed in all the alkaloid rich plant parts. Juliprosopine was pre-eminet alkaloid in leaf, whereas pod and flower displayed copious amounts of julifloridine.

关键词: DART-MS, chemical fingerprint, Prosopis juliflora, piperidine alkaloids, juliprosopine, julifloridine

Shachi SINGH, Sanjay Kumar VERMA. Study of the distribution profile of piperidine alkaloids in various parts of Prosopis juliflora by the application of Direct Analysis in Real Time Mass Spectrometry (DART-MS)[J]. Natural Products and Bioprospecting, 2012, 2(5): 206-209.

References

[1] Al-Humaid, A. I.; Warrag, O. A. J. Arid Environ. 1998, 38, 237-243.
[2] Ahmad, V. U.; Sultana, A.; Qazi, S. J. Nat. Prod. 1989, 5, 497-501.
[3] Ahmad, V. U.; Basha, A.; Haque, W. Z. Naturforsch. 1978, 33, 347-348.
[4] Daetwyler, P.; Ott-Longoni, R.; Schopp, E.; Hesse, M. Helvet. Chim. Acta. 1981, 64, 1959-1963
[5] Nakano, H.; Nakajima, E.; Hiradate, H.; Fujii, Y.; Yamada, K.; Shigemori, H.; Hasegawa, A. Phytochemstry 2004, 65, 587-591.
[6] Shankarmurthy, P.; Siddiqui, S. J. Sci. Ind. Res. 1988, 7, 188-192.
[7] Singh, S.; Swapnil; Verma, S. K. Inter. J. Pharma. Sci. Res. 2011, 2, 114-120.
[8] Kanthasamy, A.; Subramanian, S.; Govindasamy, S. Indian Drugs 1989, 26, 390-394.
[9] Kandasamy, A.; William, S.; Govindasamy, S. Cur. Sci. 1989, 58, 142-144.
[10] Nakano, H.; Nakajima, E.; Hiradate, S.; Fujii, Y.; Yamada, K.; Shigemori, H.; Hasegawa, K. Plant Growth Regul. 2004, 44, 207-210.
[11] Silva, A. M. M.; Silva, A. R.; Pinheiro, A. M.; Freitas, S. R. V. B.; Silva, V. D. A.; Souza, C. S.; Hughes, J. B.; El-Bacha, R. S.; Costa, M. F. D.; Velozo, E. S.; Tardy, M.; Costa, S. L. Toxicon 2007, 49, 601-614.
[12] Batatinha, M. J. M.[Doctoral Thesis]. Hannover:Veterinary Medicine University, 1997.
[13] Roschek, B. J.; Fink, R. C.; McMichael, M. D.; Alberte, R. S. Phytochemstry 2009, 70, 1255-1261.
[14] Cody, R. B.; Laramee, J. A.; Durst, H. D. Anal. Chem. 2005, 77, 2297-2302.
[15] Kubec, R.; Cody, R. B.; Dane, A. J.; Musah, R. A.; Schraml, J.; Vattekkatte, A.; Block, E. J. Agric. Food Chem. 2010, 58, 1121-1128.
[16] Kim, H. J.; Jang, Y. P. Phytochem. Anal. 2009, 20, 372-377.
[17] Banerjee, S.; Madhusudanan, K. P.; Khanuja, S. P.; Chattopadhyay, S. K. Biomed. Chromatogr. 2008, 22, 250-253.
[18] Madhusudanan, K. P.; Banerjee, S.; Khanuja, S. P.; Chattopadhyay, S. K. Biomed. Chromatogr. 2008, 22, 596-600.
[19] Stermitz, F. R.;. Tawara, J. N.; Boeckl, M.; Pomeroy, M.; Foderaro, T. A.; Todd, F. G. Phytochemstry 1994, 35, 951-953.
[20] Todd, F. G.; Stermitz, F. R.; Blohkin, A. V. Phytochemstry 1995, 40, 401-406.
[21] Elisabetsky, E.; Costa-Campos, L. Alternat. Med. 2006, 3, 39-48.
[22] Ratle, G.; Monseur, X.; Das, B.C.; Yassi, J.; Khuong-Huu, Q.; Goutarel, R. Bulletin de la Société chimique de France 1966, 9, 2945-2947.
[23] Shetty, B. V.; Singh, V. Flora of rajasthan, Vol 3, Botanical Survey of India, Calcutta, 1993.
[24] Ott-Longoni, R.; Viswanathan, N.; Hesse, M. Helvet. Chim. Acta. 1980, 63, 2119-2129.

Study of the distribution profile of piperidine alkaloids in various parts of Prosopis juliflora by the application of Direct Analysis in Real Time Mass Spectrometry (DART-MS)

Study of the distribution profile of piperidine alkaloids in various parts of Prosopis juliflora by the application of Direct Analysis in Real Time Mass Spectrometry (DART-MS)

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