Growth inhibition of human acute lymphoblastic CCRF-CEM leukemia cells by medicinal plants of the West-Canadian Gwich' in Native Americans

doi:10.1007/s13659-012-0013-4

Natural Products and Bioprospecting ›› 2012, Vol. 2 ›› Issue (1): 35-40.DOI: 10.1007/s13659-012-0013-4

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Growth inhibition of human acute lymphoblastic CCRF-CEM leukemia cells by medicinal plants of the West-Canadian Gwich' in Native Americans

Katharina DEEG^a, Tolga EICHHORN^b, Gladys ALEXIE^c, Nadine KRETSCHMER^d, Kai ANDERSCH^e, Rudolf BAUER^d, Thomas EFFERTH^b

a Institute of Pharmacy and Molecular Biotechnology, University of Heidelberg, Heidelberg, Germany
b Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Mainz, Germany
c Fort McPerson, Northwest Territories, Canada
d Institute of Pharmaceutical Sciences, Department Pharmacognosy, Karl-Franzens University, Graz, Austria
e Wilderness International, Dresden(Germany) and Stony Plain(Alberta, Canada

Received:2012-02-12 Revised:2012-02-16 Online:2018-02-11 Published:2012-02-24

Growth inhibition of human acute lymphoblastic CCRF-CEM leukemia cells by medicinal plants of the West-Canadian Gwich' in Native Americans

Katharina DEEG^a, Tolga EICHHORN^b, Gladys ALEXIE^c, Nadine KRETSCHMER^d, Kai ANDERSCH^e, Rudolf BAUER^d, Thomas EFFERTH^b

a Institute of Pharmacy and Molecular Biotechnology, University of Heidelberg, Heidelberg, Germany
b Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Mainz, Germany
c Fort McPerson, Northwest Territories, Canada
d Institute of Pharmaceutical Sciences, Department Pharmacognosy, Karl-Franzens University, Graz, Austria
e Wilderness International, Dresden(Germany) and Stony Plain(Alberta, Canada

通讯作者: Thomas EFFERTH,E-mail:efferth@uni-mainz.de

Abstract

Abstract: The Gwich' in, which belong to the Athapaskan language group of Native Americans live in the borderland between Alaska and Canada. We analyzed 29 medicinal plants of this tribe for their growth inhibitory activity against CCRF-CEM T-cell acute lymphoblastic leukemia(T-ALL) cells. The anti-leukemic activity of these plants has not been investigated as yet. Considering the poor cure rates of some ALL forms, there might be a great potential for medicinal plants as resource for natural products to treat T-ALL. We found that the hexane extracts of three plants revealed considerable growth inhibition on CCRF-CEM cells. The 50% inhibition concentrations(IC₅₀) were 6.63±0.03 μg/ml for Cladina mitis, 8.65±0.38 μg/ml for Picea mariana(needles), and 9.67 ±1.36 μg/ml for Artemisia frigida. Further investigations are required to isolate the active constituents of these plants.

Key words: cytotoxicity, herbs, leukemia, medicinal plants, pharmacognosy

摘要： The Gwich' in, which belong to the Athapaskan language group of Native Americans live in the borderland between Alaska and Canada. We analyzed 29 medicinal plants of this tribe for their growth inhibitory activity against CCRF-CEM T-cell acute lymphoblastic leukemia(T-ALL) cells. The anti-leukemic activity of these plants has not been investigated as yet. Considering the poor cure rates of some ALL forms, there might be a great potential for medicinal plants as resource for natural products to treat T-ALL. We found that the hexane extracts of three plants revealed considerable growth inhibition on CCRF-CEM cells. The 50% inhibition concentrations(IC₅₀) were 6.63±0.03 μg/ml for Cladina mitis, 8.65±0.38 μg/ml for Picea mariana(needles), and 9.67 ±1.36 μg/ml for Artemisia frigida. Further investigations are required to isolate the active constituents of these plants.

关键词: cytotoxicity, herbs, leukemia, medicinal plants, pharmacognosy

Katharina DEEG, Tolga EICHHORN, Gladys ALEXIE, Nadine KRETSCHMER, Kai ANDERSCH, Rudolf BAUER, Thomas EFFERTH. Growth inhibition of human acute lymphoblastic CCRF-CEM leukemia cells by medicinal plants of the West-Canadian Gwich' in Native Americans[J]. Natural Products and Bioprospecting, 2012, 2(1): 35-40.

References

[1] Smith, H. I. National Museum of Canada Bulletin. 1928, 56, 445-522.
[2] Chandler, R. F.;Freeman, L.; Hooper, S. N. J. Ethnopharmacol. 1979, 1, 49-68.
[3] Gottesfeld, L. J. J. Ethnobiol. 1994, 14, 185-210.
[4] Gottesfeld, L. J.; Anderson, B. J. Ethnobiol. 1988, 8, 13-33.
[5] Turner, N. J. Food plants of British Columbia Indians. Part 2. Interior People. handbook 36, BC Provincial Museum, Victoria, 1978, pp 14-19.
[6] Turner, N. J.; Hebda, R. J. J. Ethnopharmacol. 1990, 29, 59-72.
[7] Ritch-Krc, E. M.; Thomas, S.; Turner, N. J.; Towers, G. H. J. Ethnopharmacol. 1996, 52, 85-94.
[8] Moerman, D. E. Native American Ethnobotany. Timber Press,Portland, London, 9th printing. 2010.
[9] Moerman, D. E.; Estabrook, G. F. J. Ethnopharmacol. 2003, 87, 51-59.
[10] Runge, M. S. (ed.) Principles of molecular medicine. Humana Press:Totowa, NJ. 2nd ed. 2006.
[11] Pui, C. H.; Evans W. E. N. Engl. J. Med. 2006, 354, 166-178.
[12] Pui, C. H.; Robison, L. L.; Look, A. T. Lancet 2008, 371, 1030-1043.
[13] Silverman, L. B.; Sallan, S. E. Curr. Opin. Hematol. 2003, 10, 290-296.
[14] Jeha, S. Semin. Hematol. 2009, 46, 76-88.
[15] Pui, C. H.; Cheng, C.; Leung, W.; Rai, S. N.; Rivera, G. K.; Sandlund, J. T.; Ribeiro, R. C.; Relling, M. V.; Kun, L. E.; Evans, W. E.; Hudson, M. M. N. Engl. J. Med. 2003, 349, 640-649.
[16] Oeffinger, K. C.; Hudson, M. M. CA Cancer J. Clin. 2004, 54, 208-236.
[17] Efferth, T.; Sauerbrey, A.; Olbrich, A.; Gebhart, E.; Rauch, P.; Weber, H. O.; Hengstler, J. G.; Halatsch, M. E.; Volm, M.; Tew, K. D.; Ross, D. D.; Funk, J. O. Mol. Pharmacol. 2003, 64, 382-394.
[18] Efferth, T.; Li, P. C.; Konkimalla, V. S.; Kaina, B. Trends Mol. Med. 2007, 13, 353-361.
[19] Newman, D. J.; Cragg, G. M. J. Nat. Prod. 2007, 70, 461-477.
[20] Hay, R. J.; Williams, C. D.; Macy, M. L.; Lavappa, K. S. Am. Rev. Respir. Dis. 1982, 125, 222-232.
[21] Kofler, R. Histochem. Cell Biol. 2000, 114, 1-7.
[22] Efferth, T.; Davey, M.; Olbrich, A.; Rücker, G.; Gebhart, E.; Davey, R. Blood Cells Mol. Dis. 2002, 28, 160-168.
[23] Lambrou, G. I.; Vlahopoulos, S.; Papathanasiou, C.; Papanikolaou, M.; Karpusas, M.; Zoumakis, E.; Tzortzatou-Stathopoulou, F. Leuk Res. 2009, 33, 1684-1695.
[24] Mackinnon, A.; Pojar, J.; Coupe, R. Plants of the Northern British Columbia. Expanded 2nd ed. Lone Pine Publishing. 1999.
[25] Andre, A.; Fehr, A. Gwich'in Ethnobotany:Plants Used by the Gwich'in for Food, Medicine, Shelter and Tools. Tsiigehtchic, North West Territories, Canada:Gwich'in Social and Cultural Institute. 2001.
[26] Page, B. Barrenland Beauties, Showy Plants of the Canadian Arctic. Yellowknife, North West Territories, Canada:Outcrop, The Northern Publishers. 2004.
[27] Scudiero, D. A.; Shoemaker, R. H.; Paull, K. D.; Monks, A.; Tierney, S.; Nofziger, T. H.; Currens, M. J.; Seniff, D.; Boyd, M. R. Cancer Res. 1988, 48, 4827-4833.
[28] Konkimalla, V. B.; Efferth, T. Biochem. Pharmacol. 2010, 80, 39-49.
[29] Lopes-Lutz, D.; Alviano, D. S.; Alviano, C. S.; Kolodziejczyk, P. P. Phytochemistry 2008, 69, 1732-1738.
[30] Wang, Q. H.; Ao, W. L. J.; Wang, X. L.; Bao, X. H.; Wang, J. H. J. Asian Nat. Prod. Res. 2010, 12, 950-954.
[31] http://www.ars-grin.gov/cgi-bin/duke/farmacy2.pl. (accession date:February 12th, 2012)

Growth inhibition of human acute lymphoblastic CCRF-CEM leukemia cells by medicinal plants of the West-Canadian Gwich' in Native Americans

Growth inhibition of human acute lymphoblastic CCRF-CEM leukemia cells by medicinal plants of the West-Canadian Gwich' in Native Americans

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