Structural characterizations and α-glucosidase inhibitory activities of four Lepidium meyenii polysaccharides with different molecular weights

doi:10.1007/s13659-023-00384-1

应用天然产物 ›› 2023, Vol. 13 ›› Issue (3): 18-18.DOI: 10.1007/s13659-023-00384-1

Structural characterizations and α-glucosidase inhibitory activities of four Lepidium meyenii polysaccharides with different molecular weights

Luan Wen^1,2, Zhou-Wei Wu^1,2, Li-Wu Lin^1,2, Abdulbaset Al-Romaima^1,2, Xing-Rong Peng^1,2, Ming-Hua Qiu^1,2

1. State Key Laboratory of Phytochemistry and Plant Resources in West China, Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, Yunnan, People’s Republic of China;
2. University of Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

收稿日期:2023-05-05 出版日期:2023-06-24 发布日期:2023-07-18
通讯作者: Ming-Hua Qiu,E-mail:mhchiu@mail.kib.ac.cn
基金资助:
The research work was financially supported by the National Natural Science Foundation of China (No. 31872675), as well as the Cooperation Project with DR PLANT Company (2023).

Structural characterizations and α-glucosidase inhibitory activities of four Lepidium meyenii polysaccharides with different molecular weights

Luan Wen^1,2, Zhou-Wei Wu^1,2, Li-Wu Lin^1,2, Abdulbaset Al-Romaima^1,2, Xing-Rong Peng^1,2, Ming-Hua Qiu^1,2

1. State Key Laboratory of Phytochemistry and Plant Resources in West China, Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, Yunnan, People’s Republic of China;
2. University of Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

Received:2023-05-05 Online:2023-06-24 Published:2023-07-18
Contact: Ming-Hua Qiu,E-mail:mhchiu@mail.kib.ac.cn
Supported by:
The research work was financially supported by the National Natural Science Foundation of China (No. 31872675), as well as the Cooperation Project with DR PLANT Company (2023).

摘要/Abstract

摘要： Four polysaccharides (MCPa, MCPb, MCPc, MCPd) were obtained from Lepidium meyenii Walp. Their structures were characterized by chemical and instrumental methods including total sugar, uronic acid and protein content determination, UV, IR and NMR spectroscopy, as well as monosaccharide composition determination and methylation analyses. Four polysaccharides were a group of glucans with different molecular weights ranging from 3.12 to 14.4 kDa, and shared a similar backbone chain consisting of (1→4)-glucose linkages with branches attached to C-3 and C-6. Furthermore, bioactivity assay showed that MCPs had concentration-dependent inhibitory activity on α-glucosidase. MCPb (Mw=10.1 kDa) and MCPc (Mw=5.62 kDa) with moderate molecular weights exhibited higher inhibitory activity compared with MCPa and MCPd.

关键词: Lepidium meyenii, Polysaccharide, Molecular weight, Structural characterization, α-Glucosidase inhibition

Abstract: Four polysaccharides (MCPa, MCPb, MCPc, MCPd) were obtained from Lepidium meyenii Walp. Their structures were characterized by chemical and instrumental methods including total sugar, uronic acid and protein content determination, UV, IR and NMR spectroscopy, as well as monosaccharide composition determination and methylation analyses. Four polysaccharides were a group of glucans with different molecular weights ranging from 3.12 to 14.4 kDa, and shared a similar backbone chain consisting of (1→4)-glucose linkages with branches attached to C-3 and C-6. Furthermore, bioactivity assay showed that MCPs had concentration-dependent inhibitory activity on α-glucosidase. MCPb (Mw=10.1 kDa) and MCPc (Mw=5.62 kDa) with moderate molecular weights exhibited higher inhibitory activity compared with MCPa and MCPd.

Key words: Lepidium meyenii, Polysaccharide, Molecular weight, Structural characterization, α-Glucosidase inhibition

Luan Wen, Zhou-Wei Wu, Li-Wu Lin, Abdulbaset Al-Romaima, Xing-Rong Peng, Ming-Hua Qiu. Structural characterizations and α-glucosidase inhibitory activities of four Lepidium meyenii polysaccharides with different molecular weights[J]. 应用天然产物, 2023, 13(3): 18-18.

参考文献

1 Muthusamy S, Udayakumar GP, Narala VR. Recent advances in the extraction and characterization of seed polysaccharides, and their bioactivities: a review. Bioact Carbohydr Diet Fibre. 2021;26:100276.
2 Zhou S, Huang G. Preparation, structure and activity of polysaccharide phosphate esters. Biomed Pharmacother. 2021;144:112332.
3 Song Y, Li S, Gong H, Yip RCS, Chen H. Biopharmaceutical applications of microbial polysaccharides as materials: a review. Int J Biol Macromol. 2023;239:124259.
4 Luo JH, Li J, Shen ZC, Lin XF, Chen AQ, Wang YF, et al. Advances in health-promoting effects of natural polysaccharides: regulation on Nrf2 antioxidant pathway. Front Nutr. 2023;10:1102146.
5 Guo Y, Chen X, Gong P, Li Z, Wu Y, Zhang J, et al. Advances in the mechanisms of polysaccharides in alleviating depression and its complications. Phytomedicine. 2023;109:154566.
6 Zhao B, Zhao JY, Lv MS, Li XY, Wang JH, Yue ZR, et al. Comparative study of structural properties and biological activities of polysaccharides extracted from Chroogomphus rutilus by four different approaches. Int J Biol Macromol. 2021;188:215-25.
7 Todorova V, Ivanov K, Ivanova S. Comparison between the biological active compounds in plants with adaptogenic properties (Rhaponticum carthamoides, Lepidium meyenii, Eleutherococcus senticosus and Panax ginseng). Plants. 2021;11:64.
8 Lee HW, Lee MS, Qu F, Lee JW, Kim E. Maca (Lepidium meyenii Walp.) On semen quality parameters: a systematic review and meta-analysis. Front Pharmacol. 2022;13:934740.
9 da Silva Leitão Peres N, Cabrera Parra Bortoluzzi L, Medeiros Marques LL, Formigoni M, Fuchs RHB, Droval AA, et al. Medicinal effects of peruvian maca (Lepidium meyenii): a review. Food Funct. 2020;11:83-92.
10 Zha SH, Zhao QS, Chen JJ, Wang LW, Zhang GF, Zhang H, et al. Extraction, purification and antioxidant activities of the polysaccharides from maca (Lepidium meyenii). Carbohydr Polym. 2014;111:584-7.
11 Chen XF, Liu YY, Cao MJ, Zhang LJ, Sun LC, Su WJ, et al. Hypoxia tolerance and fatigue relief produced by Lepidium meyenii and its water-soluble polysaccharide in mice. Food Sci Technol Res. 2016;22:611-21.
12 Wang W, Zou Y, Li Q, Mao RW, Shao XJ, Jin D, et al. Immunomodulatory effects of a polysaccharide purified from Lepidium meyenii Walp. On macrophages. Process Biochem. 2016;51:542-53.
13 Zhang M, Wang G, Lai F, Wu H. Structural characterization and immunomodulatory activity of a novel polysaccharide from Lepidium meyenii. J Agric Food Chem. 2016;64:1921-31.
14 He JC, Li RW, Zhu HY. The effects of polysaccharides from Maca (Lepidium meyenii Walp.) On exhaustive exercise-induced oxidative damage in rats. Biomed Res. 2017;28:122-8.
15 Li J, Sun Q, Meng Q, Wang L, Xiong W, Zhang L. Anti-fatigue activity of polysaccharide fractions from Lepidium meyenii Walp. (maca). Int J Biol Macromol. 2017;95:1305-11.
16 Li S, Hao L, Kang Q, Cui Y, Jiang H, Liu X, et al. Purification, characterization and biological activities of a polysaccharide from Lepidium meyenii leaves. Int J Biol Macromol. 2017;103:1302-10.
17 Tang W, Jin L, Xie L, Huang J, Wang N, Chu B, et al. Structural characterization and antifatigue effect in vivo of Maca (Lepidium meyenii Walp) Polysaccharide. J Food Sci. 2017;82:757-64.
18 Zhang M, Wu W, Ren Y, Li X, Tang Y, Min T, et al. Structural characterization of a novel polysaccharide from Lepidium meyenii (Maca) and analysis of its regulatory function in macrophage polarization in vitro. J Agric Food Chem. 2017;65:1146-57.
19 Wang CX, Xu L, Huang LQ, Li XR, Han W, Liu DQ, et al. Optimization of Maca polysaccharide extraction process and its chemo-protective effects on cyclophosphamide-induced mice. J Food Process Eng. 2018;41:e12856.
20 Guo T, Yang Y, Gao M, Qu Y, Guo X, Liu Y, et al. Lepidium meyenii Walpers polysaccharide and its cationic derivative re-educate tumor-associated macrophages for synergistic tumor immunotherapy. Carbohydr Polym. 2020;250:116904.
21 Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Anal Chem. 1956;28:350-6.
22 Li J, Kisara K, Danielsson S, Lindström ME, Gellerstedt G. An improved methodology for the quantification of uronic acid units in xylans and other polysaccharides. Carbohydr Res. 2007;342:1442-9.
23 Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248-54.
24 Liu J, Shang F, Yang Z, Wu M, Zhao J. Structural analysis of a homogeneous polysaccharide from Achatina fulica. Int J Biol Macromol. 2017;98:786-92.
25 Sun X, Ai C, Wen C, Peng H, Yang J, Cui Y, et al. Inhibitory effects of fucoidan from Laminaria japonica against some pathogenic bacteria and SARS-CoV-2 depend on its large molecular weight. Int J Biol Macromol. 2023;229:413-21.
26 Zeng W, Chen L, Xiao Z, Li Y, Ma J, Ding J, et al. Comparative study on the structural properties and bioactivities of three different molecular weights of Lycium barbarum polysaccharides. Molecules. 2023;28:701.
27 Nicely WB. Infrared spectra of carbohydrates. In: Melville L, Wolfrom R, editors. Stuart Tipson. Advances in carbohydrate chemistry. New York: Academic; 1957. pp. 13-33.
28 Ciucanu I. Per-O-methylation reaction for structural analysis of carbohydrates by mass spectrometry. Anal Chim Acta. 2006;576:147-55.
29 York WS, Darbill AG, McNeil M, Stevenson TT, Albersheim P. Isolation and characterization of plant cell walls and cell wall components. Meth Enzymol. 1986;118:3-40.
30 Jansson PE, Kenne L, Schweda E. N.m.r. and conformational studies of the methyl glycosides of some 1,2- and 1,3-linked disaccharides. J Chem Soc Perkin Trans. 1988;1:2729-36.
31 Yuan Q, Zhao L, Cha Q, Sun Y, Ye H, Zeng X. Structural characterization and immunostimulatory activity of a homogeneous polysaccharide from Sinonovacula constricta. J Agric Food Chem. 2015;63:7986-94.
32 Li K, Cui LJ, Cao YX, Li SY, Shi LX, Qin XM, et al. UHPLC Q-exactive MS-based serum metabolomics to explore the effect mechanisms of immunological activity of Astragalus polysaccharides with different molecular weights. Front Pharmacol. 2020;11:595692.
33 Xu Z, Li X, Feng S, Liu J, Zhou L, Yuan M, et al. Characteristics and bioactivities of different molecular weight polysaccharides from camellia seed cake. Int J Biol Macromol. 2016;91:1025-32.
34 Sun XY, Wang JM, Ouyang JM, Kuang L. Antioxidant activities and repair effects on oxidatively damaged HK-2 cells of tea polysaccharides with different molecular weights. Oxid Med Cell Longev. 2018;2018:5297539.
35 Tsukamoto T, Hattori M, Sakabe M, Haginaka J. Determination of the molecular mass of new L-fucose-containing glycosaminoglycan and its distribution by high-performance gel-permeation chromatography with laser light-scattering detection. Anal Sci. 2001;17:555-8.
36 Fu D, O’Neill RA. Monosaccharide composition analysis of oligosaccharides and glycoproteins by high-performance liquid chromatography. Anal Biochem. 1995;227:377-84.
37 Ciucanu I, Costello CE. Elimination of oxidative degradation during the per-O-methylation of carbohydrates. J Am Chem Soc. 2003;125:16213-9.
38 Fang DS, Cheng CR, Qiu MH, Peng XR. Diverse meroterpenoids with α-glucosidase inhibitory activity from Ganoderma cochlear. Fitoterapia. 2023;165:105420.

[1]	Zhou-Wei Wu, Xue-Fang Zhao, Chen-Xi Quan, Xiao-Cui Liu, Xin-Yu Tao, Yu-jie Li, Xing-Rong Peng, Ming-Hua Qiu. Structure-function insights of natural Ganoderma polysaccharides: advances in biosynthesis and functional food applications[J]. 应用天然产物, 2025, 15(2): 15-15.
[2]	Ni Huang, Yi-Na Yang, Jia Huang, Hui-Yan Shao, Yan-Lang Li, Shi-Hui Qin, Han-Fen Li, Xiao-Jiang Shen, Liu Yang, Jiang-Miao Hu. Structure characterization and immunoactivity on dendritic cells of two neutral polysaccharides from Dictyophora rubrovalvata[J]. 应用天然产物, 2024, 14(6): 52-52.
[3]	Srijan Banerjee, Gustavo Cabrera-Barjas, Jaime Tapia, João Paulo Fabi, Cedric Delattre, Aparna Banerjee. Characterization of Chilean hot spring-origin Staphylococcus sp. BSP3 produced exopolysaccharide as biological additive[J]. 应用天然产物, 2024, 14(2): 3-3.
[4]	Yan-Jie Huang, Xing-Rong Peng, Ming-Hua Qiu. Progress on the Chemical Constituents Derived from Glucosinolates in Maca (Lepidium meyenii)[J]. 应用天然产物, 2018, 8(6): 405-412.
[5]	Daniela Batista, Pedro L. Falé, Maria L. Serralheiro, Maria E. Araújo, Paulo J. A. Madeira, Carlos Borges, Isabel Torgal, Margarida Goulart, Jorge Justino, Alice Martins, Amélia P. Rauter. New In Vitro Studies on the Bioprofile of Genista tenera Antihyperglycemic Extract[J]. 应用天然产物, 2015, 5(6): 277-285.

Structural characterizations and α-glucosidase inhibitory activities of four Lepidium meyenii polysaccharides with different molecular weights

Structural characterizations and α-glucosidase inhibitory activities of four Lepidium meyenii polysaccharides with different molecular weights

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 5

编辑推荐

Metrics

本文评价