Evaluation of Terpenes' Degradation Rates by Rumen Fluid of Adapted and Non-adapted Animals

doi:10.1007/s13659-020-00289-3

应用天然产物 ›› 2021, Vol. 11 ›› Issue (3): 307-314.DOI: 10.1007/s13659-020-00289-3

• ORIGINAL ARTICLES • 上一篇下一篇

Evaluation of Terpenes' Degradation Rates by Rumen Fluid of Adapted and Non-adapted Animals

I. Poulopoulou¹, I. Hadjigeorgiou²

1 Faculty of Science and Technology, Free University of Bolzano, Univeritätsplatz 5, 39100 Bozen-Bolzano, Italy;
2 Department of Nutritional Physiology and Feeding, Faculty of Animal Science and Aquaculture, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, Greece

收稿日期:2020-10-12 修回日期:2020-11-13 出版日期:2021-06-24 发布日期:2021-06-04
通讯作者: I. Poulopoulou

Evaluation of Terpenes' Degradation Rates by Rumen Fluid of Adapted and Non-adapted Animals

I. Poulopoulou¹, I. Hadjigeorgiou²

1 Faculty of Science and Technology, Free University of Bolzano, Univeritätsplatz 5, 39100 Bozen-Bolzano, Italy;
2 Department of Nutritional Physiology and Feeding, Faculty of Animal Science and Aquaculture, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, Greece

Received:2020-10-12 Revised:2020-11-13 Online:2021-06-24 Published:2021-06-04
Contact: I. Poulopoulou

摘要/Abstract

摘要： The aim of the present study was to evaluate terpenes degradation rate in the rumen fluid from adapted and non-adapted animals. Four castrated healthy animals, two rams and two bucks, were used. Animals were daily orally dosed for 2 weeks with 1 g of each of the following terpenes, α-pinene, limonene and β-caryophyllene. At the end of each week, rumen fluid (RF) samples were assayed in vitro for their potential to degrade terpenes over time. For each animal, a 10 mL reaction medium (RM) at a ratio 1:9 (v/v) was prepared and a terpenes solution at a concentration of 100 μg/ml each, was added in each RM tube. Tubes were incubated at 39℃ under anaerobic conditions and their contents sampled at 0, 2, 4, 8, 21 and 24 h. RF could degrade terpenes as it was shown by the significantly (P < 0.05) higher overall degradation rates. Individual terpene degradation rates, were significantly (P < 0.05) higher in week 5 for limonene and marginally (P=0.083) higher also in week 5 for α-pinene. In conclusion, the findings of the present preliminary study suggest that terpenes can be degraded in the rumen fluid.

关键词: Terpenes, Degradation, Rumen, Sheep, Goats

Abstract: The aim of the present study was to evaluate terpenes degradation rate in the rumen fluid from adapted and non-adapted animals. Four castrated healthy animals, two rams and two bucks, were used. Animals were daily orally dosed for 2 weeks with 1 g of each of the following terpenes, α-pinene, limonene and β-caryophyllene. At the end of each week, rumen fluid (RF) samples were assayed in vitro for their potential to degrade terpenes over time. For each animal, a 10 mL reaction medium (RM) at a ratio 1:9 (v/v) was prepared and a terpenes solution at a concentration of 100 μg/ml each, was added in each RM tube. Tubes were incubated at 39℃ under anaerobic conditions and their contents sampled at 0, 2, 4, 8, 21 and 24 h. RF could degrade terpenes as it was shown by the significantly (P < 0.05) higher overall degradation rates. Individual terpene degradation rates, were significantly (P < 0.05) higher in week 5 for limonene and marginally (P=0.083) higher also in week 5 for α-pinene. In conclusion, the findings of the present preliminary study suggest that terpenes can be degraded in the rumen fluid.

Key words: Terpenes, Degradation, Rumen, Sheep, Goats

I. Poulopoulou, I. Hadjigeorgiou. Evaluation of Terpenes' Degradation Rates by Rumen Fluid of Adapted and Non-adapted Animals[J]. 应用天然产物, 2021, 11(3): 307-314.

I. Poulopoulou, I. Hadjigeorgiou. Evaluation of Terpenes' Degradation Rates by Rumen Fluid of Adapted and Non-adapted Animals[J]. Natural Products and Bioprospecting, 2021, 11(3): 307-314.

参考文献

1. E. Abilleira, M. de Renobales, A.I. Najera, M. Virto, J.C.R.D. Gordoa, F.J. Perez-Elortondo, M. Albisu, L.J.R. Barron, Food Chem. 120, 1162-1169 (2010)
2. I. Poulopoulou, E. Zoidis, T. Massouras, I. Hadjigeorgiou, J. Anim. Physiol. Anim. Nutr. 25, 1411-1418 (2012)
3. I. Poulopoulou, E. Zoidis, S. Avramidou, T. Massouras, I. Hadjigeorgiou, Emir. J. Food and Agr. 31, 980-985 (2020)
4. L. Zhang, Y. Wang, P. Zou, X. Pan, H. Zhang, W. Chen, J. US Chin. Med. Sci. 2, 59-72 (2005)
5. A. Adams, J.C.R. Demyttenaere, N. De Kimpe, Food Chem. 80, 525-534 (2003)
6. M. Clericuzio, G. Alagona, C. Ghio, L. Toma, J. Org. Chem. 65, 6910-6916 (2000)
7. L.B. Welch, C.J. Rederson, J. Range Manage. 34, 497-500 (1981)
8. J.H. Langenheim, J. Chem. Ecol. 20, 1223-1280 (1994)
9. R. Bodas, N. Prieto, R. García-González, S. Andrés, F.J. Giráldez, S. López, Anim. Feed Sci. Technol. 176, 78-93 (2012)
10. G. Cobellis, M. Trabalza-Marinucci, Z. Yu, Sci. Total Environ. 545-546, 556-568 (2016)
11. M. Malecky, L.P. Broudiscou, J. Anim. Sci. 87, 1366-1373 (2009)
12. M. Malecky, L.P. Broudiscou, P. Schmidely, Anim. Feed Sci. Technol. 154, 24-35 (2009)
13. J. Harder, C. Probian, Appl. Environ. Microbiol. 61, 3804-3808 (1995)
14. P.B. Hylemon, J. Harder, FEMS Microbiol. Rev. 22, 475-488 (1998)
15. M. Spanghero, C. Zanfi, E. Fabbro, N. Scicutella, C. Camellini, Anim. Feed Sci. Technol. 145, 364-374 (2008)
16. M. Busquet, S. Calsamiglia, A. Ferret, C. Kamel, Anim. Feed Sci. Technol. 123-124, 597-613 (2005)
17. H.K. Oh, T. Sakai, M.B. Jones, W.M. Longhurst, Appl. Microbiol. 15, 777-784 (1967)
18. L.K. Cluff, L.B. Welch, J.C. Pederson, J.D. Brotherson, J. Range Manage. 35, 192-194 (1982)
19. L.E. Dziba, J.O. Hall, F.D. Provenza, J. Chem. Ecol. 32, 391-408 (2006)
20. G. Turner, J. Gershenzon, E.E. Nielson, J.E. Froehlich, R. Croteau, Plant Physiol. 120, 879-886 (1999)
21. G. Tornambe, A. Cornu, P. Pradel, N. Kondjoyan, A.P. Carnat, M. Petit, B. Martin, J. Dairy Sci. 89, 2309-2319 (2006)
22. J.J. Villalba, F.D. Provenza, Animal 1, 1360-1370 (2007)
23. R.R. Hofmann, Oecologia 78, 443-457 (1989)
24. A.R. Castillo-González, M.E. Burrola-Barraza, J. DomínguezViveros, A. Chávez-Martínez, Arch. Med. Vet. 46, 349-361 (2014)
25. L.P. Broudiscou, A. Cornu, A. Rouzeau, J. Sci. Food Agric. 87, 1653-1658 (2007)
26. R.E. Estell, S.A. Utsumi, A.F. Cibils, Anim. Feed Sci. Technol. 158, 104-109 (2010)
27. P.G. Toral, L. Bernard, A. Belenguer, J. Rouel, G. Hervás, Y. Chilliard, P. Frutos, J. Dairy Sci. 99, 301-326 (2016)
28. P.G. Toral, G. Hervás, D. Carreño, H. Leskinen, A. Belenguer, K.J. Shingfield, P. Frutos, J. Dairy Sci. 100, 6187-6198 (2017)
29. F.M. McIntosh, P. Williams, R. Losa, R.J. Wallace, D.A. Beever, C.J. Newbold, Appl. Environ. Microbiol. 69, 5011-5014 (2003)
30. L. Castillejos, S. Calsamiglia, A. Ferret, J. Dairy Sci. 89, 2649-2658 (2006)
31. A.J. Duncan, P. Frutos, S.A. Young, Anim. Sci. 65, 451-455 (1997)
32. M. Busquet, S. Calsamiglia, A. Ferret, C. Kamel, J. Dairy Sci. 89, 761-771 (2006)
33. S.H.H. Chow, D. Salazar, I.A. Hakim, Biomark. Prev. 11, 1472-1476 (2002)
34. S.K.S. Raghuvansi, R. Prasad, A.S. Mishra, O.H. Chaturvedi, M.K. Tripathi, A.K. Misra, B.L. Saraswat, R.C. Jakhmola, Bioresour. Technol. 98, 511-517 (2007)
35. L. Castillejos, S. Calsamiglia, A. Ferret, R. Losa, Anim. Feed Sci. Technol. 119, 29 (2005)
36. Statgraphics^®, Version 11, Manugistics, Inc. 2115, East Jefferson str. Rockville, MA 2852, USA (2000)

[1]	Zhe-Wei Yu, Bang-Ping Cai, Su-Zhi Xie, Yi Zhang, Wen-Hui Wang, Shun-Zhi Liu, Yan-Lin Bin, Qi Chen, Mei-Juan Fang, Rong Qi, Ming-Yu Li, Ying-Kun Qiu. Compounds from Agathis dammara exert hypoglycaemic activity by enhancing glucose uptake: lignans, terpenes and others[J]. 应用天然产物, 2024, 14(3): 23-23.
[2]	Inga Dammann, Claudia Keil, Iris Hardewig, Elżbieta Skrzydlewska, Michał Biernacki, Hajo Haase. Effects of combined cannabidiol (CBD) and hops (Humulus lupulus) terpene extract treatment on RAW 264.7 macrophage viability and inflammatory markers[J]. 应用天然产物, 2023, 13(3): 19-19.
[3]	Suhad A. A. Al-Salihi, Fabrizio Alberti. Naturally Occurring Terpenes: A Promising Class of Organic Molecules to Address Influenza Pandemics[J]. 应用天然产物, 2021, 11(4): 405-419.
[4]	Yi-Jie Zhai, Jian-Nan Li, Yu-Qi Gao, Lin-Lin Gao, Da-Cheng Wang, Wen-Bo Han, Jin-Ming Gao. Structurally Diverse Sesquiterpenoids with Anti-neuroinflammatory Activity from the Endolichenic Fungus Cryptomarasmius aucubae[J]. 应用天然产物, 2021, 11(3): 325-332.
[5]	Jia-Huan Shang, Guo-Wei Xu, Hong-Tao Zhu, Dong Wang, Chong-Ren Yang, Ying-Jun Zhang. Anti-inflammatory and Cytotoxic Triterpenes from the Rot Roots of Panax notoginseng[J]. 应用天然产物, 2019, 9(4): 287-295.
[6]	Kennedy D. Nyongbela, Fidele Ntie-Kang, Thomas R. Hoye, Simon M. N. Efange. Antiparasitic Sesquiterpenes from the Cameroonian Spice Scleria striatinux and Preliminary In Vitro and In Silico DMPK Assessment[J]. 应用天然产物, 2017, 7(3): 235-247.
[7]	Joseph Sakah Kaunda, Ying-Jun Zhang. The Genus Carissa: An Ethnopharmacological, Phytochemical and Pharmacological Review[J]. 应用天然产物, 2017, 7(2): 181-199.
[8]	Rong-Hua Yin, Zhen-Zhu Zhao, Xu Ji, Ze-Jun Dong, Zheng-Hui Li, Tao Feng, Ji-Kai Liu. Steroids and Sesquiterpenes From Cultures of the Fungus Phellinus igniarius[J]. 应用天然产物, 2015, 5(1): 17-22.
[9]	Can-Bin Ouyang, Xiao-Man Liu, Qi Liu, Jie Bai, Hou-Yong Li, Yuan Li, Qiu-Xia Wang, Dong-Dong Yan, Lian-Gang Mao, Aocheng Cao, Mei-Xia Guo. Toxicity Assessment of Cadinene Sesquiterpenes from Eupatorium adenophorum in Mice[J]. 应用天然产物, 2015, 5(1): 29-36.
[10]	Zi-Ming Chen, Qiong-Ying Fan, Xia Yin, Xiao-Yan Yang, Zheng-Hui Li, Tao Feng, Ji-Kai Liu. Three New Humulane Sesquiterpenes from Cultures of the Fungus Antrodiella albocinnamomea[J]. 应用天然产物, 2014, 4(4): 207-211.
[11]	Xia Yin, Tao Feng, Zheng-Hui Li, Ying Leng, Ji-Kai Liu. Five New Guanacastane-Type Diterpenes from Cultures of the Fungus Psathyrella candolleana[J]. 应用天然产物, 2014, 4(3): 149-155.
[12]	Chun-Miao LI, Hui-Chun GENG, Ming-Ming LI, Gang XU, Tie-Jun LING, Hong-Bo QIN. Total synthesis of 1-oxomiltirone via Suzuki coupling[J]. 应用天然产物, 2013, 3(3): 117-120.
[13]	Yuan-Feng YANG, Jie-Qing LIU, Lei SHI, Zhong-Rong LI, Ming-Hua QIU. New jatropholane-type diterpenes from Jatropha curcas cv. Multiflorum CY Yang[J]. 应用天然产物, 2013, 3(3): 99-102.
[14]	Xia YIN, Tao FENG, Zheng-Hui LI, Jia SU, Yan LI, Ning-Hua TAN, Ji-Kai LIU. Chemical investigation on the cultures of the fungus Xylaria carpophila[J]. 应用天然产物, 2011, 1(2): 75-80.

Evaluation of Terpenes' Degradation Rates by Rumen Fluid of Adapted and Non-adapted Animals

Evaluation of Terpenes' Degradation Rates by Rumen Fluid of Adapted and Non-adapted Animals

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 14

编辑推荐

Metrics

本文评价