Phytochemical Analysis and Antifungal Activity of Extracts from Leaves and Fruit Residues of Brazilian Savanna Plants Aiming Its Use as Safe Fungicides

doi:10.1007/s13659-016-0101-y

应用天然产物 ›› 2016, Vol. 6 ›› Issue (4): 195-204.DOI: 10.1007/s13659-016-0101-y

Phytochemical Analysis and Antifungal Activity of Extracts from Leaves and Fruit Residues of Brazilian Savanna Plants Aiming Its Use as Safe Fungicides

Caroline Alves Breda^1,2, Alessandra Marcon Gasperini^1,2, Vera Lucia Garcia³, Karin Maia Monteiro⁴, Giovana Anceski Bataglion⁵, Marcos Nogueira Eberlin⁵, Marta Cristina Teixeira Duarte²

1. School of Food Engineering, State University of Campinas, UNICAMP, Monteiro Lobato Street, 80, Barão Geraldo, Campinas, São Paulo 13083-862, Brazil;
2. Microbiology Division of Research Center for Chemistry, Biology and Agriculture-CPQBA, University of Campinas, UNICAMP, Alexandre Cazelatto Street, 999, Betel, Paulínia, São Paulo 13148-218, Brazil;
3. Organic Chemistry and Pharmaceutical Division of Research Center for Chemistry, Biology and Agriculture-CPQBA, University of Campinas, UNICAMP, Alexandre Cazelatto Street, 999, Betel, Paulínia, São Paulo 13148-218, Brazil;
4. Pharmacology and Toxicology Division of Research Center for Chemistry, Biology and Agriculture-CPQBA, University of Campinas, UNICAMP, Alexandre Cazelatto Street, 999, Betel, Paulínia, São Paulo 13148-218, Brazil;
5. ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry, State University of Campinas, UNICAMP, Campinas, São Paulo 13084-971, Brazil

收稿日期:2016-02-22 修回日期:2016-05-04 出版日期:2016-08-24 发布日期:2018-02-08
通讯作者: Caroline Alves Breda,e-mail:carol_breda@yahoo.com.br
基金资助:
We are grateful to São Paulo Research Foundation for the financial support of this research and for the fellowships awarded to C.A.B.(FAPESP No.2011/19057-7) and G. A. B.(FAPESP No.2012/21395-0) and to Study Group of Cerrado Agroindustrial Processes (GEPPAC) of the Federal University of Grande Dourados to provide laboratories for plant material processing step

Phytochemical Analysis and Antifungal Activity of Extracts from Leaves and Fruit Residues of Brazilian Savanna Plants Aiming Its Use as Safe Fungicides

Caroline Alves Breda^1,2, Alessandra Marcon Gasperini^1,2, Vera Lucia Garcia³, Karin Maia Monteiro⁴, Giovana Anceski Bataglion⁵, Marcos Nogueira Eberlin⁵, Marta Cristina Teixeira Duarte²

1. School of Food Engineering, State University of Campinas, UNICAMP, Monteiro Lobato Street, 80, Barão Geraldo, Campinas, São Paulo 13083-862, Brazil;
2. Microbiology Division of Research Center for Chemistry, Biology and Agriculture-CPQBA, University of Campinas, UNICAMP, Alexandre Cazelatto Street, 999, Betel, Paulínia, São Paulo 13148-218, Brazil;
3. Organic Chemistry and Pharmaceutical Division of Research Center for Chemistry, Biology and Agriculture-CPQBA, University of Campinas, UNICAMP, Alexandre Cazelatto Street, 999, Betel, Paulínia, São Paulo 13148-218, Brazil;
4. Pharmacology and Toxicology Division of Research Center for Chemistry, Biology and Agriculture-CPQBA, University of Campinas, UNICAMP, Alexandre Cazelatto Street, 999, Betel, Paulínia, São Paulo 13148-218, Brazil;
5. ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry, State University of Campinas, UNICAMP, Campinas, São Paulo 13084-971, Brazil

Received:2016-02-22 Revised:2016-05-04 Online:2016-08-24 Published:2018-02-08
Supported by:
We are grateful to São Paulo Research Foundation for the financial support of this research and for the fellowships awarded to C.A.B.(FAPESP No.2011/19057-7) and G. A. B.(FAPESP No.2012/21395-0) and to Study Group of Cerrado Agroindustrial Processes (GEPPAC) of the Federal University of Grande Dourados to provide laboratories for plant material processing step

摘要/Abstract

摘要： The increasing demand for safe food without preservatives or pesticides residues has encouraged several studies on natural products with antifungal activity and low toxicity. In this study, ethanolic extracts from leaves and fruit residues (peel and seeds) of three Brazilian savanna species (Acrocomia aculeata, Campomanesia adamantium and Caryocar brasiliense) were evaluated against phytopathogenic fungi. Additionally, the most active extract was chemically characterized by ESI-MS and its oral acute toxicity was evaluated. Extracts from C. brasiliense (pequi) peel and leaves were active against Alternaria alternata, Alternaria solani and Venturia pirina with minimal inhibitory concentrations between 350 and 1000 μg/mL. When incorporated in solid media, these extracts extended the lag phase of A. alternata and A. solani and reduced the growth rate of A. solani. Pequi peel extract showed better antifungal activity and their ESI-MS analysis revealed the presence of substances widely reported as antifungal such as gallic acid, quinic acid, ellagic acid, glucogalin and corilagin. The oral acute toxicity was relatively low, being considered safe for use as a potential natural fungicide.

关键词: Brazilian savanna fruits, Residues, Natural fungicides, Phytopathogens

Abstract: The increasing demand for safe food without preservatives or pesticides residues has encouraged several studies on natural products with antifungal activity and low toxicity. In this study, ethanolic extracts from leaves and fruit residues (peel and seeds) of three Brazilian savanna species (Acrocomia aculeata, Campomanesia adamantium and Caryocar brasiliense) were evaluated against phytopathogenic fungi. Additionally, the most active extract was chemically characterized by ESI-MS and its oral acute toxicity was evaluated. Extracts from C. brasiliense (pequi) peel and leaves were active against Alternaria alternata, Alternaria solani and Venturia pirina with minimal inhibitory concentrations between 350 and 1000 μg/mL. When incorporated in solid media, these extracts extended the lag phase of A. alternata and A. solani and reduced the growth rate of A. solani. Pequi peel extract showed better antifungal activity and their ESI-MS analysis revealed the presence of substances widely reported as antifungal such as gallic acid, quinic acid, ellagic acid, glucogalin and corilagin. The oral acute toxicity was relatively low, being considered safe for use as a potential natural fungicide.

Key words: Brazilian savanna fruits, Residues, Natural fungicides, Phytopathogens

Caroline Alves Breda, Alessandra Marcon Gasperini, Vera Lucia Garcia, Karin Maia Monteiro, Giovana Anceski Bataglion, Marcos Nogueira Eberlin, Marta Cristina Teixeira Duarte. Phytochemical Analysis and Antifungal Activity of Extracts from Leaves and Fruit Residues of Brazilian Savanna Plants Aiming Its Use as Safe Fungicides[J]. 应用天然产物, 2016, 6(4): 195-204.

参考文献

1. I. Camele, L. Altieri, L. de Martino, V. de Feo, E. Mancini, G.L. Rana, Int. J. Mol. Sci. 13, 2290-2300 (2012)
2. R. Braghini, C.R. Pozzi, S. Aquino, L.O. Rocha, B. Corrêa, Appl. Radiat. Isot. 67, 1622-1628 (2009)
3. J. Tian, X. Ban, H. Zeng, B. Huang, J. He, Y. Wang, Food Control 22, 1992-1999 (2011)
4. D.J. de Rodríguez, R.R. García, F.D.H. Castillo, C.N.A. Gonza-lez, A.S. Galindo, J.A.V. Quintanilla, L.E.M. Zuccolotto, Ind. Crops Prod. 34, 960-966 (2011)
5. H.F. Maswada, S. Abdallah, J. Biol. Sci. 23, 1698-1705 (2013)
6. F.M. Silva, J.E. Paula, L.S. Espindola, Mycoses 52, 511-517 (2009)
7. B.R. Santos, R. Paiva, J.L.D. Dombroski, C. Martinotto, R.C. Nogueira, A.A.N. Silva, Bol. Agrop. Univ. Federal de Lavras. 64, 5-33 (2004)
8. M.R. Silva, D.B.C.L. Lacerda, G.G. Santos, D.M.O. Martins, Ciênc. Rural. 38, 1790-1793 (2008)
9. T.S. Agostini-Costa, D.B. da Silva, R.F. Vieira, S.M. Sano, F.R. Ferreira, in Frutas nativas da regiao Centro-Oeste do Brasil, ed. by R.F. Vieira, T.S. Agostini-Costa, D.B. da Silva, S.M. Sano, F.R. Ferreira (Embrapa, Brasília, 2010), pp. 15-30
10. X.S. Passos, S.C. Santos, P.H. Ferri, O.F.L. Fernandes, T.F. Paula, A.C.F. Garcia, M.R.R. Silva, Rev. Soc. Bras. Med. Trop. 35, 623-627 (2002)
11. I.D. Coutinho, C.A.L. Cardoso, N. Ré-Poppi, A.M. Melo, M.C. Vieira, N.K. Honda, R.G. Coelho, Braz. J. Pharm. Sci. 45, 767-776 (2009)
12. A. Dapkevicius, R. Venskutonis, T.A.V. Beek, J. Linssen, J. Sci. Food Agric. 77, 140-146 (1998)
13. M.C.T. Duarte, G.M. Figueira, A. Sartoratto, V.L.G. Rehder, C. Delarmelina, J. Ethnopharmacol. 97, 305-311 (2005)
14. H.A. Bashir, A.A. Abu-Goukh, Food Chem. 80, 557-563 (2003)
15. R.M. Donlan, J.W. Costerton, Clin. Microbiol. Rev. 15, 167-193 (2002)
16. K. Baert, A. Valero, B. de Meulenaer, S. Samapundo, M.M. Ahmed, L. Bo, J. Debevere, F. Devlieghere, Int. J. Food Microbiol. 118, 139-150 (2007)
17. G.J.E. Nychas, in New methods of food preservation, ed. by G.W. Gould (Blackie Academic and Professional, New York, 1995), pp. 58-69
18. M. de la Luz Cadiz-Gurrea, S. Fernandez-Arroyo, J. Joven, A. Segura-Carretero, Food Res. Int. 50, 197-204 (2013)
19. S.T. Huang, C.Y. Wang, R.C. Yang, H.T. Wu, S.H. Yang, Y.C. Cheng, J.H.S. Pang, Altern. Med. 2011, 215035 (2011)
20. J.H. Lee, J.V. Johnson, S.T. Talcott, J. Agric. Food Chem. 53, 6003-6010 (2005)
21. R. Roesler, R.R. Catharino, L.G. Malta, M.N. Eberlin, G. Pastore, Food Chem. 110, 711-717 (2008)
22. J. Jayamani, G. Shanmugam, Eur. J. Med. Chem. 85, 352-358 (2014)
23. W. Bhouri, J. Boubaker, I. Skandrani, K. Ghedira, L.C. Ghedira, Cancer Cell Int. 12, 1-8 (2012)
24. E. Osorio, M. Flores, D. Hernandez, J. Ventura, R. Rodríguez, C.N. Aguilar, Ind. Crops Prod. 31, 153-157 (2010)
25. A. Barco, S. Benetti, C.D. Risi, P. Marchetti, G.P. Pollini, V. Zanirato, Tetrahedron-Asymmetry 8, 3515-3545 (1997)
26. J. Chen, M.L. Yang, J. Zeng, K. Gao, Phytochem. Lett. 6, 41-45 (2013)
27. J. Tian, Y. Xie, Y. Zhao, C. Li, S. Zhao, Luminescence 26, 296-304 (2011)
28. J.D. N'Guessan, A.P. Bidié, B.N. Lenta, B. Weniger, P. André, F. Guédé-Guina, African. J. Biotechnol. 6, 1685-1689 (2007)
29. M.S. Pinto, F.M. Lajolo, M.I. Genovese, Food Chem. 107, 1629-1635 (2008)
30. P. Buzzini, P. Arapitsas, M. Goretti, E. Branda, B. Turchetti, P. Pinelli, F. Ieri, A. Romani, Mini Rev. Med. Chem. 8, 1179-1187 (2008)
31. K.P. Latté, H. Kolodziej, Z. Naturforschung C. 55, 467-472 (2000)
32. Z. Wang, Q.Y. Guo, X.J. Zhang, X. Li, W.T. Li, X.T. Ma, L.J. Ma, Int. J. Mol. Sci. 15, 9762-9779 (2014)
33. A. Perera, D. Appleton, L.H. Ying, S. Elendran, U.D. Palanisamy, Sep. Purif. Technol. 98, 145-149 (2012)
34. T. Taguri, T. Tanaka, I. Kouno, Biol. Pharm. Bull. 27, 1965-1969 (2004)
35. Organization for Economic Co-operation and Development, OECD guideline for testing of chemicals-guideline no 420 (2001)
36. S.K. El-Arini, H. Leuenberger, Pharm. Acta Helv. 73, 89-94 (1998)
37. National Commitee for Clinical Laboratory Standards, NCCLS document M38-A (2002)
38. M. Gougoli, K.P. Koutsoumanis, Int. J. Food Microbiol. 161, 231-239 (2013)

Phytochemical Analysis and Antifungal Activity of Extracts from Leaves and Fruit Residues of Brazilian Savanna Plants Aiming Its Use as Safe Fungicides

Phytochemical Analysis and Antifungal Activity of Extracts from Leaves and Fruit Residues of Brazilian Savanna Plants Aiming Its Use as Safe Fungicides

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics

本文评价