Morphological Effects of Natural Products on Schizosaccharomyces pombe Measured by Imaging Flow Cytometry

doi:10.1007/s13659-014-0004-8

应用天然产物 ›› 2014, Vol. 4 ›› Issue (1): 27-35.DOI: 10.1007/s13659-014-0004-8

• Original article • 上一篇下一篇

Morphological Effects of Natural Products on Schizosaccharomyces pombe Measured by Imaging Flow Cytometry

Joel Heisler¹, Lindsay Elvir¹, Farah Barnouti¹, Erica Charles¹, Tom D. Wolkow², Radha Pyati¹

1. University of North Florida, Jacksonville, FL, USA;
2. University of Colorado at Colorado Springs, Colorado Springs, CO, USA

收稿日期:2013-11-27 修回日期:2014-01-14 出版日期:2014-02-24 发布日期:2018-02-11
通讯作者: Radha Pyati,e-mail:radha.pyati@unf.edu
基金资助:
The authors acknowledge financial support from the University of North Florida. They also thank the NCI/DTP Open Chemical Repository(http://dtp.cancer.gov), for making the natural products available.

Morphological Effects of Natural Products on Schizosaccharomyces pombe Measured by Imaging Flow Cytometry

Joel Heisler¹, Lindsay Elvir¹, Farah Barnouti¹, Erica Charles¹, Tom D. Wolkow², Radha Pyati¹

1. University of North Florida, Jacksonville, FL, USA;
2. University of Colorado at Colorado Springs, Colorado Springs, CO, USA

Received:2013-11-27 Revised:2014-01-14 Online:2014-02-24 Published:2018-02-11
Supported by:
The authors acknowledge financial support from the University of North Florida. They also thank the NCI/DTP Open Chemical Repository(http://dtp.cancer.gov), for making the natural products available.

摘要/Abstract

摘要： Gaining a full understanding of the mechanisms of action of natural products as therapeutic agents includes observing the effects of natural products on cellular morphology, because abnormal cellular morphology is an important aspect of cellular transformations that occur as part of disease states. In this study a set of natural products was examined in search of small molecules that influence the cylindrical morphology of fission yeast Schizosaccharomyces pombe. Imaging flow cytometry of large populations of S. pombe exposed to natural products captured cell images and revealed changes in mean length and aspect ratio of cells. Several natural products were found to alter S. pombe's morphology relative to control, in terms of elongating cells, shrinking them, or making them more round. These results may facilitate future investigations into methods by which cells establish and maintain specific shapes.

关键词: Schizosaccharomyces pombe, Morphology, Natural products, Imaging flow cytometry, Aspect ratio, Fission yeast

Abstract: Gaining a full understanding of the mechanisms of action of natural products as therapeutic agents includes observing the effects of natural products on cellular morphology, because abnormal cellular morphology is an important aspect of cellular transformations that occur as part of disease states. In this study a set of natural products was examined in search of small molecules that influence the cylindrical morphology of fission yeast Schizosaccharomyces pombe. Imaging flow cytometry of large populations of S. pombe exposed to natural products captured cell images and revealed changes in mean length and aspect ratio of cells. Several natural products were found to alter S. pombe's morphology relative to control, in terms of elongating cells, shrinking them, or making them more round. These results may facilitate future investigations into methods by which cells establish and maintain specific shapes.

Key words: Schizosaccharomyces pombe, Morphology, Natural products, Imaging flow cytometry, Aspect ratio, Fission yeast

Joel Heisler, Lindsay Elvir, Farah Barnouti, Erica Charles, Tom D. Wolkow, Radha Pyati. Morphological Effects of Natural Products on Schizosaccharomyces pombe Measured by Imaging Flow Cytometry[J]. 应用天然产物, 2014, 4(1): 27-35.

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Morphological Effects of Natural Products on Schizosaccharomyces pombe Measured by Imaging Flow Cytometry

Morphological Effects of Natural Products on Schizosaccharomyces pombe Measured by Imaging Flow Cytometry

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