Metabolite profiles of Paragliomastix luzulae (formerly named as Acremonium striatisporum) KMM 4401 and its co-cultures with Penicillium hispanicum KMM 4689

doi:10.1007/s13659-024-00459-7

应用天然产物 ›› 2024, Vol. 14 ›› Issue (5): 38-38.DOI: 10.1007/s13659-024-00459-7

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Metabolite profiles of Paragliomastix luzulae (formerly named as Acremonium striatisporum) KMM 4401 and its co-cultures with Penicillium hispanicum KMM 4689

G. B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 159 Prospect 100-Letiya Vladivostoka, Vladivostok, 690022, Russian Federation

收稿日期:2024-03-29 出版日期:2024-10-24 发布日期:2024-10-14
通讯作者: Anton N. Yurchenko,E-mail:yurchenkoan@piboc.dvo.ru
基金资助:
This research was funded by a grant from the Ministry of Science and Higher Education of the Russian Federation, 15.BRK.21.0004 (Contract No. 075-15-2021-1052).

Metabolite profiles of Paragliomastix luzulae (formerly named as Acremonium striatisporum) KMM 4401 and its co-cultures with Penicillium hispanicum KMM 4689

G. B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 159 Prospect 100-Letiya Vladivostoka, Vladivostok, 690022, Russian Federation

Received:2024-03-29 Online:2024-10-24 Published:2024-10-14
Contact: Anton N. Yurchenko,E-mail:yurchenkoan@piboc.dvo.ru
Supported by:
This research was funded by a grant from the Ministry of Science and Higher Education of the Russian Federation, 15.BRK.21.0004 (Contract No. 075-15-2021-1052).

摘要/Abstract

摘要： The marine holothurian-derived fungal strain KMM 4401 has been identified as Paragliomastix luzulae using 28S rDNA, ITS regions and the partial TEF1 gene sequences. The metabolite profile of the fungal culture was studied by UPLC-MS technique. The strain KMM 4401 is a source of various virescenoside-type isopimarane glycosides suggested as chemotaxonomic feature for this fungal species. Also Px. luzulae KMM 4401 was proposed as possible source of new bioactive secondary metabolites especially antimicrobials. Moreover, the co-cultures of Px. luzulae KMM 4401 with another marine fungus Penicillium hispanicum KMM 4689 inoculated simultaneously or after two weeks were investigated by same way. It was shown, that P. hispanicum KMM 4689 suppressed the production of most of Px. luzulae KMM 4401 metabolites. On the other hand, the co-cultivation of P. hispanicum KMM 4689 and Px. luzulae KMM 4401 resulted in increasing of production of main deoxyisoaustamide alkaloids of P. hispanicum KMM 4689 on 50-190%.

关键词: Paragliomastix luzulae, 28S rDNA, ITS, TEF1, Phylogeny, Identification, Penicillium hispanicum, Co-cultivation

Abstract: The marine holothurian-derived fungal strain KMM 4401 has been identified as Paragliomastix luzulae using 28S rDNA, ITS regions and the partial TEF1 gene sequences. The metabolite profile of the fungal culture was studied by UPLC-MS technique. The strain KMM 4401 is a source of various virescenoside-type isopimarane glycosides suggested as chemotaxonomic feature for this fungal species. Also Px. luzulae KMM 4401 was proposed as possible source of new bioactive secondary metabolites especially antimicrobials. Moreover, the co-cultures of Px. luzulae KMM 4401 with another marine fungus Penicillium hispanicum KMM 4689 inoculated simultaneously or after two weeks were investigated by same way. It was shown, that P. hispanicum KMM 4689 suppressed the production of most of Px. luzulae KMM 4401 metabolites. On the other hand, the co-cultivation of P. hispanicum KMM 4689 and Px. luzulae KMM 4401 resulted in increasing of production of main deoxyisoaustamide alkaloids of P. hispanicum KMM 4689 on 50-190%.

Key words: Paragliomastix luzulae, 28S rDNA, ITS, TEF1, Phylogeny, Identification, Penicillium hispanicum, Co-cultivation

Sofya S. Starnovskaya, Liliana E. Nesterenko, Roman S. Popov, Natalya N. Kirichuk, Viktoria E. Chausova, Ekaterina A. Chingizova, Artur R. Chingizov, Marina P. Isaeva, Ekaterina A. Yurchenko, Anton N. Yurchenko. Metabolite profiles of Paragliomastix luzulae (formerly named as Acremonium striatisporum) KMM 4401 and its co-cultures with Penicillium hispanicum KMM 4689[J]. 应用天然产物, 2024, 14(5): 38-38.

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Metabolite profiles of Paragliomastix luzulae (formerly named as Acremonium striatisporum) KMM 4401 and its co-cultures with Penicillium hispanicum KMM 4689

Metabolite profiles of Paragliomastix luzulae (formerly named as Acremonium striatisporum) KMM 4401 and its co-cultures with Penicillium hispanicum KMM 4689

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