Effects of Extracellular ATP on the Chitosan-induced Changes in ROS Levels and PAL Activity

doi:10.7525/j.issn.1673-5102.2021.04.014

Abstract

Abstract:

In this experiment， tobacco BY-2 suspension cells were used as materials to investigate the effects of extracellular ATP on the chitosan-induced changes of ROS（reactive oxygen species）level and PAL（phenylalanine ammonia-lyase）activity. The results showed that the tobacco suspension cells mixed with chitosan 5-20 μg·mL^-1 increased the intracellular ROS level at adose-dependent manner， and the PAL activity also increased and reached to the maximum at 15 μg·mL^-1 chitosan， and then decreased slightly. There was not significant difference in ROS levels and PAL activity in tobacco suspension cells by adding 10-40 μmol·L^-1 exogenous ATP. The extracellular ATP（eATP） level gradually decreased with the concentration of chitosan increased. The effects of eATP on the chitosan-induced change of ROS level and PAL activity were further analyzed. The results showed that the exogenous ATP 20 μmol·L^-1 added to chitosan-induced tobacco suspension cells effectively alleviated the increases of ROS level and PAL activity in cells. These results showed that effects of extracellular ATP can affect the chitosan-induced changes in ROS level and PAL activity in cells.

Key words: chitosan, extracellular ATP, ROS, PAL

CLC Number:

Q945.79

De-Li YANG, Juan-Juan WANG, Hai-Long PANG, Kun SUN, Han-Qing FENG. Effects of Extracellular ATP on the Chitosan-induced Changes in ROS Levels and PAL Activity[J]. Bulletin of Botanical Research, 2021, 41(4): 588-595.

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