Separation and Purification of Three Active Components from Dracocephalum tanguticum Maxim. by High-Speed Counter-current Chromatography Combined with Semi-Preparative High Performance Liquid Chromatography

doi:10.7525/j.issn.1673-5102.2022.02.010

Bulletin of Botanical Research ›› 2022, Vol. 42 ›› Issue (2): 252-258.doi: 10.7525/j.issn.1673-5102.2022.02.010

• Phytochemistry • Previous Articles Next Articles

Separation and Purification of Three Active Components from Dracocephalum tanguticum Maxim. by High-Speed Counter-current Chromatography Combined with Semi-Preparative High Performance Liquid Chromatography

Jiaben Zhou¹, Caidan Rezeng², Maoxian Re¹, Cairang Limao¹^,³()

^1.College of Life Sciences，Qinghai Normal University，Xining 810008
^2.College of Pharmacy，Qinghai University for Nationalities，Xining 810007
^3.College of Nationalities，Qinghai Normal University，Xining 810008

Received:2021-03-18 Online:2022-03-20 Published:2022-02-22
Contact: Cairang Limao E-mail:1224863256@qq.com
About author:Zhou Jiaben（1996—），male，postgraduate，research direction for Medicinal Phytochemistry．
Supported by:
National Natural Science Foundation of China(31660086);Key Laboratory of Medicinal Animal and Plant Resources in Qinghai-Tibet Plateau of Qinghai Province(2017-ZJ-Y13)

Abstract

Abstract:

An efficient method was established for the separation and preparation of chlorogenic acid， pedaliin-6″-acetate and rosmarinic acid from Dracocephalum tanguticum Maxim.. The chlorogenic acid， pedaliin-6″-acetate and rosmarinic acid was enriched by semi-preparative high performance liquid chromatography（SP-HPLC） from ethyl acetate extract， and then separated and purification by high-speed counter-current chromatography（HSCCC） and preparative high performance liquid chromatography（Pre-HPLC）. A total of 54 mg of chlorogenic acid， 150 mg of pedaliin-6″- acetate and 200 mg of rosmarnic acid were obtained from ethyl acetate extract with purities of 96.9%， 97.9% and 95.1% by HPLC analysis， respectively. Their structures were identified by nuclear magnetic resonance spectrum. The result show that this method is suitable for the isolation and purification of chlorogenic acid， pedaliin-6″-acetate and rosmarnic acid from D. tanguticum. It also avoids the disadvantages of traditional separation methods such as many operations， large reagent consumption and non-recyclability， provides a reference basis for the study of separating of active components and preparation reference substance from D.tanguticum.

Key words: high-speed counter-current chromatography, preparative high-performance liquid chromatography, Dracocephalumtanguticum Maxim., chlorogenic acid, Pedaliin-6″-acetate, rosmarnic acid

CLC Number:

S132

Jiaben Zhou, Caidan Rezeng, Maoxian Re, Cairang Limao. Separation and Purification of Three Active Components from Dracocephalum tanguticum Maxim. by High-Speed Counter-current Chromatography Combined with Semi-Preparative High Performance Liquid Chromatography[J]. Bulletin of Botanical Research, 2022, 42(2): 252-258.

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Separation and Purification of Three Active Components from Dracocephalum tanguticum Maxim. by High-Speed Counter-current Chromatography Combined with Semi-Preparative High Performance Liquid Chromatography

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