Abstract: We discussed the effect of light-emitting diodes(LEDs) light qualities on the multiplication and rooting of Vaccinium bracteatum. The application of light quality control will offer scientific references to improve the efficiency and quality of V.bracteatum tissue culture. The light quality change was controlled by the LEDs, and the fluorescent was set as control. Different rations of red and blue lights set were listed below:100% red, 70% red+30% blue, 50% red+50% blue, 30% red+70% blue and 100% blue. The study released that the red light was benefit for new shoot multiplication and leaf area increase, and limited for chlorophyll synthesis significantly; and blue light was benefit for adventitious roots form and limited for shoot formation. The complex of red and blue lights was best for stem growth, biomass accumulation, and root development in V.bracteatum tissue culture. In addition, with the light quality of 70% red+30% blue, the shoot induction rate, number of shoots, number of leaves, fresh and dry weights of the shoots with buds reached the maximum values. During the rooting stage, the light quality of 50% red+50% blue was best for rooting rate, root number and root length in culture tissues. The ratio of chlorophyll a/b got the highest value at pure blue light condition, and the number is 1.46 times than that at red light condition. The complex lights could increase carotenoid concentrations in V.bracteatum tissues. Therefore, LEDs could improve V.bracteatum shoot multiplication and rooting, and LEDs could be an optimization than the fluorescent.

Key words: light-emitting diodes(LEDs), light quality, Vaccinium bracteatum, shoot multiplication, rooting