Abstract: For reducing hyperhydric rate and improving rooting percentage of in vitro regenerated shoots from Scutellariae baicalensis Georgi, with the regenerated shoots induced from aseptic seedling stems, we studied the effect of N⁶-Benzyladenine(6-BA), sucrose, agar and paclobotrazol(PP333) on hyperhydricity of regenerated shoots in S.baicalensis and the effects of IBA on rooting of regenerated shoots. The hyperhydric rate significantly decreased with lower 6-BA concentration on the medium. The lowest hyperhydric rate and the highest multiplication rate were on the medium containing 0.2 mg·L^-1 6-BA. With the sucrose concentration increasing, the hyperhydric rate of S.baicalensis adventitious shoots was reduced, while their multiplication coefficient was also reduced. The best multiplication coefficient in vitro shoots without hyperhydricity were found on the medium containing 25 g·L^-1 sucrose. On the medium containing 7.5 g·L^-1 agar, the hyperhydric rate of S.baicalensis adventitious shoots was the lowest with higher proliferation coefficient. The medium containing PP333 positively affected the hyperhydric occurrence of regenerated shoots. With the increase of PP333 concentration on the medium, the hyperhydric rate was decreased gradually and in vitro shoots were stronger and healthier. The optimal rooting was got from the medium containing 0.1 mg·L^-1 IBA, with the rooting rate of 100% and seven roots per shoot. The survival rate of rooting plantlets was over 95% after transferred to soil.

Key words: Scutellariae baicalensis Georgi, adventitious shoots, hyperhydricity, rooting