Cloning and Expression Analysis of S-Adenosine Methionine Decarboxylase(ThSAMDC) Gene from Tamarix ramosissima

doi:10.7525/j.issn.1673-5102.2018.01.016

Abstract

Abstract: S-adenosyl-L-methionine decarboxylase(SAMDC) is a rate-limiting enzyme that regulates the physiological and biochemical processes of plants by participating in the metabolic pathway of polyamines. The cDNA of S-Adenosine Methionine Decarboxylase gene(named ThSAMDC) was isolated and cloned by analyzing the transcriptome data of Tamarix hispida. The full-length of ThSAMDC is 2 085 bp, with three open reading frames, tiny ORF(tORF), upstream ORF(uORF) and main ORF(mORF). The mORF was 1 107 bp encoding 369 amino acids. The relative molecular weight and isoelectric points(PI) of the putative protein were 40.34 kD and 4.72. ThSAMDC-encoded protein has a number of strong hydrophilic regions, no obvious transmembrane region. Compared to the amino acid multiple sequence alignment of several other species, ThSAMDC includes two highly conserved domains: the proenzyme cleavage site(LSESSLF) and the PEST domain(TIHVTPEDGFSYAS) associated with the rapid degradation of the protein. Phylogenetic tree analysis shows that ThSAMDC has higher sequence similarity of 77% identities to the Spinach(SoSAMDC). Quantitative real-time PCR assay revealed that the mRNA level of ThSAMDC was significantly up-regulated under NaCl, PEG, ABA and CdCl₂ treatments in T.hispida, suggesting that ThSAMDC may play an important role in drought resistance, salt tolerance and other stresses.

Key words: Tamarix hispida, S-adenosylmethionine decarboxylase, stress responses, gene expression

CLC Number:

S793.5

ZHANG Yu, ZHANG Yue, ZHANG Chun-Rui, WANG Yan-Min, WANG Yu-Cheng, WANG Chao. Cloning and Expression Analysis of S-Adenosine Methionine Decarboxylase(ThSAMDC) Gene from Tamarix ramosissima[J]. Bulletin of Botanical Research, 2018, 38(1): 132-140.

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