Cloning and Expression Analysis of S-Adenosyl-L-Methionine Synthetase(ThSAMS) Gene from Tamarix hispida

doi:10.7525/j.issn.1673-5102.2019.01.014

Abstract

Abstract: A full length cDNA of a S-Adenosyl-L-Methionine Synthetase gene(named ThSAMS) was isolated from the transcriptome cDNA librarys of Tamarix hispida. The open reading frame of ThSAMS cDNA was 1 185 bp encoding 394 amino acids. The relative molecular weight of ThSAMS protein was 97.85 kDa, with isoelectric point of 5.02. Multiple alignment analysis based on the amino acids indicated that ThSAMS had high homology to SAMS proteins from different species, and the highest similarity of 95% to the Ziziphus jujuba SAMS. Quantitative real-time PCR(qRT-PCR) assay revealed that ThSAMS gene can respond to NaCl, PEG and ABA stress, suggesting that it may be involved in salt and drought stresses in T.hispida. This study provided theoretical groundwork for further exploration into the function of stress tolerance of SAMS gene in plants.

Key words: Tamarix hispida, ThSAMS gene, S-Adenosyl-L-Methionine Synthetase, gene expression

CLC Number:

S793.5

ZHANG Yue, ZHAO Xin, HOU Zheng, WANG Yan-Min, WANG Yu-Cheng, WANG Chao. Cloning and Expression Analysis of S-Adenosyl-L-Methionine Synthetase(ThSAMS) Gene from Tamarix hispida[J]. Bulletin of Botanical Research, 2019, 39(1): 113-122.

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