PagTIP1;3 Regulates Photosynthetic Physiological Characteristics of 84K Poplar in Response to Salt Stress

doi:10.7525/j.issn.1673-5102.2026.04.008

Abstract

Abstract:

Tonoplast intrinsic proteins（TIPs）， as important members of the aquaporin family， play crucial roles in plant water balance and stress responses. In this study， the function and molecular mechanism of the PagTIP1；3 gene in response to salt stress were investigated in the leaves of wild-type 84K poplar（Populus alba×P.glandulosa） and PagTIP1；3 knockout lines. The results showed that under normal conditions， knockout of the PagTIP1；3 gene led to decreases in chlorophyll content， net photosynthetic rate， and plant height. Under salt stress， the knockout lines exhibited aggravated wilting and yellowing， significantly reduced plant height， and marked decreases in photosynthetic physiological parameters， including maximum photochemical efficiency， electron transport rate， and relative chlorophyll content， compared with the wild type. Transcriptome analysis revealed that the expression patterns of key genes involved in the photosynthetic pathway（including PagLHCB7， PagGAPCP-2， PagPSY， and PagRCA） were significantly altered in the PagTIP1；3 knockout lines in response to salt stress. In summary， it is speculated that PagTIP1；3 deficiency disrupted the transcriptional response of photosynthesis-related genes in poplar under salt stress， thereby impairing photosynthetic electron transport and photoprotection capacity， disturbing growth and photosynthetic physiological homeostasis， and ultimately significantly reducing salt tolerance in poplar.

Key words: aquaporin, transcriptome, salt stress, photosynthetic characteristics, gene knockout

CLC Number:

Q943.2

Yongqi HU, Rui ZHANG, Yisen ZHANG, Yuepeng SONG. PagTIP1;3 Regulates Photosynthetic Physiological Characteristics of 84K Poplar in Response to Salt Stress[J]. Bulletin of Botanical Research, 2026, 46(4): 666-676.

Figures/Tables 8

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引物名称 Primer name	上游引物（5′→3′） Upstream primer（5′→3′）	下游引物（5′→3′） Downstream primer（5′→3′）
PagTIP1；3-KO	ACAGCAACAACAGAGCTCCTC	CGAAGGTCACAGCCGGATTC
PagLHCB7-qPCR	TCTTTCTTGCAGCGACCGAT	GGGTCAATGGACAGACCCAG
PagGAPCP-2-qPCR	TCTTGTTGAAGCTTCCCGCT	CCTGCTGGAATTGAAGTGCC
PagPSY-qPCR	TCTCGGATGTCATAGCAGGA	ATTGCCCAGACAGCTCTTCG
PagRCA-qPCR	GACAATGTTCCCGAGGACGA	GCTCAAAAGTTGGGGGTCCT
Actin	ACACGGGGAGGTAGTGACAA	CCTCCAATGGATCCTCGTTA

差异表达基因组分 DEGs	差异表达基因数量 Number of DEGs
差异表达基因组分 DEGs	根 Root	茎 Stem	叶 Leaf
上调Up-regulated	1 611	430	27
下调Down-regulated	2 321	350	2
总数Total number	3 932	780	29

GO编号 GO ID	GO注释 GO description	基因数量 Gene number	P
GO：0003700	序列特异性DNA结合转录因子活性 Transcription factor activity，sequence-specific DNA binding	32	8.92×10^-9
GO：0006355	DNA模板化转录调控 Regulation of transcription，DNA - templated	45	6.75×10^-7
GO：0016491	氧化还原酶活性 Oxidoreductase activity	28	3.14×10^-6
GO：0055114	氧化还原过程 Oxidation-reduction process	51	1.89×10^-5
GO：0005975	碳水化合物代谢过程 Carbohydrate metabolic process	36	4.27×10^-5
GO：0019752	羧酸代谢过程 Carboxylic acid metabolic process	29	7.53×10^-5
GO：0055085	跨膜转运 Transmembrane transport	38	9.16×10^-5
GO：0043169	阳离子结合 Cation binding	41	1.24×10^-4
GO：0016209	抗氧化活性 Antioxidant activity	18	2.68×10^-4
GO：0006412	翻译 Translation	25	3.95×10^-4

GO编号 GO ID	GO注释 GO description	基因数量 Gene number	P
GO：0001071	核酸结合转录因子活性 Nucleic acid binding transcription factor activity	43	7.84×10^-8
GO：0006355	DNA模板化转录调控 Regulation of transcription，DNA-templated	62	5.77×10^-5
GO：0003700	序列特异性DNA结合转录因子活性 Transcription factor activity，sequence-specific DNA binding	43	7.84×10^-8
GO：0004722	蛋白丝氨酸/苏氨酸磷酸酶活性 Protein serine/threonine phosphatase activity	14	5.23×10^-6
GO：0006470	蛋白质去磷酸化 Protein dephosphorylation	15	2.74×10^-5
GO：0005984	二糖代谢过程 Disaccharide metabolic process	10	6.35×10^-7
GO：0016052	碳水化合物分解代谢过程 Carbohydrate catabolic process	11	2.39×10^-5
GO：0009311	寡糖代谢过程 Oligosaccharide metabolic process	10	3.25×10^-6
GO：0044723	单生物体碳水化合物代谢过程 Single-organism carbohydrate metabolic process	23	1.08×10^-5
GO：2000112	细胞大分子生物合成过程调控 Regulation of cellular macromolecule biosynthetic process	62	7.83×10^-5