Analysis of the Promoter Sequence and Response Characteristics of the BpPIN3 gene in Betula platyphylla

doi:10.7525/j.issn.1673-5102.2022.04.009

Abstract

Abstract:

As an important auxin efflux carrier family in plants， PIN protein family shows extensive physiological effects in plant growth and development. In order to further understand the function of BpPIN3 and explore its response to different hormone signals and abiotic stresses during Betula platyphylla development，the BpPIN3 promoter sequence was analyzed by bioinformatics methods. Tissue expression patterns of roots， stems， leaves and apical buds of annual and biennial B. platyphylla clones were analyzed respectively. The seedlings were used as conduct hormone induction and light stress treatment with 100 μmol·L^-1 auxin^{（IAA）， 100 μmol·L^-1 gibberellin（GA₃）， 200 μmol·L^-1 abscisic acid（ABA） and long light conditions， respectively. RNA was extracted from leaves and roots of B. platyphylla at 0， 2， 4， 8， 16， 24 and 48 h after hormone treatment and at 0， 1， 3， 6， 12， 24， 48 and 72 h after light stress， and qRT-PCR was used to analyze the expression of BpPIN3. The results showed that the BpPIN3 promoter sequence contained different types of auxin response elements such as gibberellin， abscisic acid， and methyl jasmonate， as well as multiple stress-related cis-regulatory elements. BpPIN3 was expressed in many tissues of birch in different growth years， especially in leaves， and the expression of BpPIN3 in the second year was higher than that in the first year. The relative expression of BpPIN3 gene was different in different parts under different treatment conditions. IAA and GA₃ could induce the up-regulation of BpPIN3 expression in leaf. Under ABA treatment， except for 16 and 48 h， the expression pattern of BpPIN3 gene was opposite to that under IAA treatment. IAA， GA₃ and ABA could induce the expression of BpPIN3 in root， but shading stress induced the expression of BpPIN3 gene in leaf， the relative expression of BpPIN3 gene was significantly higher than that of the control（0 h） from 12 h in root， IAA， GA₃ and ABA could induce the expression of BpPIN3 in root， shading stress induced the expression of BpPIN3 gene in leaf； the relative expression of BpPIN3 gene was significantly higher than that of the control （0 h）from 12 h in root. The results suggested that BpPIN3 gene played the important regulatory role in the growth and development of B. platyphylla，and the signal transduction pathways of IAA， GA₃and ABA and plant light response.}

Key words: Betula platyphylla, BpPIN3, plant hormones, light response

CLC Number:

S792.153

Kun CHEN, Gonggui FANG, Huaizhi MU, Jing JIANG. Analysis of the Promoter Sequence and Response Characteristics of the BpPIN3 gene in Betula platyphylla[J]. Bulletin of Botanical Research, 2022, 42(4): 592-601.

References

1	BOOKER J， CHATFIELD S， LEYSER O.Auxin acts in xylem-associated or medullary cells to mediate apical dominance［J］.The Plant Cell，2003，15（2）：495-507.
2	VANNESTE S， FRIML J.Auxin：a trigger for change in plant development［J］.Cell，2009，136（6）：1005-1016.
3	BENJAMINS R， SCHERES B.Auxin：the looping star in plant development［J］.Annual Review of Plant Biology，2008，59（1）：443-465.
4	PARK J， PARK J， KIM Y，et al.GH3-mediated auxin homeostasis links growth regulation with stress adaptation response in Arabidopsis ［J］.Journal of Biological Chemistry，2007，282（13）：10036-10046.
5	SWARUP R， BENNETT M.Auxin transport：the fountain of life in plants？［J］.Development Cell，2003，5（6）：824-826.
6	PETRáSEK J， MRAVEC J， BOUCHARD R，et al.PIN proteins perform a rate-limiting function in cellular auxin efflux［J］.Science，2006，312（5775）：914-918.
7	GRUNEWALD W， FRIML J.The march of the PINs：developmental plasticity by dynamic polar targeting in plant cells［J］.The EMBO Journal，2010，29（16）：2700-2714.
8	BLILOU I， XU J， WILDWATER M，et al.The PIN auxin efflux facilitator network controls growth and patterning in Arabidopsis roots［J］.Nature，2005，433（7021）：39-44.
9	ALONSO-PERAL M M， CANDELA H， POZO J C DEL，et al.The HVE/CAND1 gene is required for the early patterning of leaf venation in Arabidopsis ［J］.Development，2006，133（19）：3755-3766.
10	LUSCHNIG C， GAXIOLA R A， GRISAFI P，et al.EIR1，a root-specific protein involved in auxin transport，is required for gravitropism in Arabidopsis thaliana ［J］.Genes Development，1998，12（14）：2175-2187.
11	CHEN Q， LIU Y， MAERE S，et al.A coherent transcriptional feed-forward motif model for mediating auxin-sensitive PIN3 expression during lateral root development［J］.Nature Communication，2015，6：8821.
12	WILLIGE B C， CHORY J.A current perspective on the role of AGCVIII kinases in PIN-mediated apical hook development［J］.Frontiers in Plant Science，2015，6：767.
13	GANGULY A， LEE S H， CHO M，et al.Differential auxin-transporting activities of PIN-FORMED proteins in Arabidopsis root hair cells［J］.Plant Physiology，2010，153（3）：1046-1061.
14	FRIML J， BENKOVá E， BLILOU I，et al. AtPIN4 mediates sink-driven auxin gradients and root patterning in Arabidopsis ［J］.Cell，2002，108（5）：661-673.
15	DING Z， WANG B， MORENO I，et al.ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis ［J］.Nature Communications，2012，3（1）：941.
16	MRAVEC J， SK?PA P， BAILLY A，et al.Subcellular homeostasis of phytohormone auxin is mediated by the ER-localized PIN5 transporter［J］.Nature，2009，459（7250）：1136-1140.
17	KEUSKAMP D H， POLLMANN S， VOESENEK L A C J，et al.Auxin transport through PIN-FORMED3（PIN3） controls shade avoidance and fitness during competition［J］.Proceedings of the National Academy of Sciences of the United States of America，2010，107（52）：22740-22744.
18	FRIML J， VIETEN A， SAUER M，et al.Efflux-dependent auxin gradients establish the apical-basal axis of Arabidopsis ［J］.Nature，2003，426（6963）：147-153.
19	DING Z， GALVáN-AMPUDIA C S， DEMARSY E，et al.Light-mediated polarization of the PIN3 auxin transporter for the phototropic response in Arabidopsis ［J］.Nature Cell Biology，2011，13（4）：447-452.
20	白羽聪，李翔宇，程占超，等.毛竹PIN基因家族的鉴定与生物信息学分析［J］.分子植物育种，2019，17（16）：5238-5247.
20	BAI Y C， LI X Y， CHENG Z C，et al.Identification and bioinformatics analysis of PIN gene family in moso bamboo （Phyllostachys edulis）［J］.Molecular Plant Breeding，2019，17（16）：5238-5247.
21	王占军，杨立伟，徐忠东，等.麻疯树PIN基因家族的鉴定与生物信息学分析［J］.分子植物育种，2015，13（5）：1111-1122.
21	WANG Z J， YANG L W， XU Z D，et al.Identification and bioinformatics analysis of the PIN gene family in Jatropha curcas ［J］.Molecular Plant Breeding，2015，13（5）：1111-1122.
22	VIAENE T， DELWICHE C F， RENSING S A，et al.Origin and evolution of PIN auxin transporters in the green lineage［J］.Trends in Plant Science，2013，18（1）：5-10.
23	LIU B B， ZHANG J， WANG L，et al.A survey of Populus PIN-FORMED family genes reveals their diversified expression patterns［J］.Journal Experimental Botany，2014，65（9）：2437-2448.
24	BARKOULAS M，HAY A， KOUGIOUMOUTZI E，et al.A developmental framework for dissected leaf formation in the Arabidopsis relative Cardamine hirsuta ［J］.Nature Genetics，2008，40（9）：1136-1141.
25	FORESTAN C， VAROTTO S.The role of PIN auxin efflux carriers in polar auxin transport and accumulation and their effect on shaping maize development［J］.Molecular Plant，2012，5（4）：787-798.
26	SHI Z， JIANG Y， HAN X，et al. SlPIN1 regulates auxin efflux to affect flower abscission process［J］.Scientific Reports，2017，7（1）：14919.
27	PATTISON R J， CATALá C.Evaluating auxin distribution in tomato（Solanum lycopersicum） through an analysis of the PIN and AUX/LAX gene families［J］.The Plant Journal，2012，70（4）：585-598.
28	XIE X D， QIN G Y， SI P，et al.Analysis of Nicotiana tabacum PIN genes identifies NtPIN4 as a key regulator of axillary bud growth［J］.Physiologia Plantarum，2017，160（2）：222-239.
29	SHEN C， YUE R， BAI Y，et al.Identification and analysis of Medicago truncatula auxin transporter gene families uncover their roles in responses to Sinorhizobium meliloti infection［J］.Plant and Cell Physiology，2015，56（10）：1930-1943.
30	SA?KO-SAWCZENKO I， ?OTOCKA B， CZARNOCKA W.Expression analysis of PIN genes in root tips and nodules of Medicago truncatula ［J］.International Journal of Molecular Sciences，2016，17（8）：1197.
31	ZHANG Y Z， HE P， YANG Z R，et al.A genome-scale analysis of the PIN gene family reveals its functions in cotton fiber development［J］.Frontiers Plant Science，2017，8：461.
32	GOU J Q， STRAUSS S H， TSAI C J，et al.Gibberellins regulate lateral root formation in Populus through interactions with auxin and other hormones［J］.The Plant Cell，2010，22（3）：623-639.
33	HAN R， WANG S， LIU C，et al.Transcriptome analysis of a multiple-branches mutant terminal buds in Betula platyphylla × B.pendula ［J］.Forests，2019，10（5）：374.
34	QU C， BIAN X Y， HAN R，et al.Expression of BpPIN is associated with IAA levels and the formation of lobed leaves in Betula pendula ‘Dalecartica’［J］.Journal of Forestry Research，2020，31（1）：87-97.
35	刘宇，徐焕文，刘桂丰，等.赤霉素GA₄₊₇处理下白桦无性系生长及差异基因表达分析［J］.林业科学研究，2017，30（1）：181-189.
35	LIU Y， XU H W， LIU G F，et al.Analysis of clonal growth and differences in gene expression of Betula platyphylla × B.pendula under GA₄₊₇ treatment［J］.Forest Research，2017，30（1）：181-189.
36	黄海娇，李慧玉，姜静. BpAP1转基因白桦中开花相关基因的时序表达［J］.东北林业大学学报，2017，45（1）：1-6.
36	HUANG H J， LI H Y， JIANG J.Quantitative expression analysis of several flowering-related genes in BpAP1 transgenic birch（Betula platyphylla × Betula pendula）［J］.Journal of Northeast Forestry University，2017，45（1）：1-6.
37	AHUJA I， DE VOS R C H， BONES A M，et al.Plant molecular stress responses face climate change［J］.Trends in Plant Science，2010，15（12）：664-674.
38	DU H， LIU H B， XIONG L Z.Endogenous auxin and jasmonic acid levels are differentially modulated by abiotic stresses in rice［J］.Frontiers in Plant Science，2013，4：397.
39	SHANI E， SALEHIN M， ZHANG Y，et al.Plant stress tolerance requires auxin-sensitive Aux/IAA transcriptional repressors［J］.Current Biology，2017，27（3）：437-444.
40	ZAHIR Z A.Substrate-dependent auxin production by Rhizobium phaseoli improves the growth and yield of Vigna radiata L.under salt sress conditions［J］.Journal of Microbiology and Biotechnology，2010，20（9）：1288-1294.
41	TANAKA H， DHONUKSHE P， BREWER P B，et al.Spatiotemporal asymmetric auxin distribution：a means to coordinate plant development［J］.Cellular and Molecular Life Sciences，2006，63（23）：2738-2754.
42	POTTERS G， PASTERNAK T P， GUISEZ Y，et al.Stress-induced morphogenic responses：growing out of trouble？［J］.Trends in Plant Science，2007，12（3）：98-105.
43	TOGNETTI V B， AKEN O VAN， MORREEL K，et al.Perturbation of indole-3-butyric acid homeostasis by the UDP-Glucosyltransferase UGT74E2 modulates Arabidopsis architecture and water stress tolerance［J］.The Plant Cell，2010，22（8）：2660-2679.
44	CASANOVA-SáEZ R， VO? U.Auxin metabolism controls developmental decisions in land plants［J］.Trends in Plant Science，2019，24（8）：741-754.
45	BRUMOS J， ROBLES L M， YUN J，et al.Local auxin biosynthesis is a key regulator of plant development［J］.Developmental Cell，2018，47（3）：306-318.e5.
46	DING Y H， MA Y Z， LIU N，et al.MicroRNAs involved in auxin signalling modulate male sterility under high-temperature stress in cotton（Gossypium hirsutum）［J］.The Plant Journal，2017，91（6）：977-994.
47	ZHANG Q， LI J J， ZHANG W J，et al.The putative auxin efflux carrier OsPIN3t is involved in the drought stress response and drought tolerance［J］.The Plant Journal，2012，72（5）：805-816.
48	FRIML J.Subcellular trafficking of PIN auxin efflux carriers in auxin transport［J］.European Journal of Cell Biology，2010，89（2/3）：231-235.
49	WANG J R， HU H， WANG G H，et al.Expression of PIN genes in rice（Oryza sativa L.）：tissue specificity and regulation by hormones［J］.Molecular Plant，2009，2（4）：823-831.
50	YUE R，TIE S， SUN T，et al.Genome-wide identification and expression profiling analysis of ZmPIN，ZmPILS，ZmLAX and ZmABCB auxin transporter gene families in maize（Zea mays L.） under various abiotic stresses［J］.PLoS One，2015，10（3）：e0118751.
51	杨蕴力，渠畅，王阳，等.白桦BpPIN5基因启动子组织定位及外源激素应答分析［J］.植物研究，2022，42（1）：104-111.
51	YANG Y L， QU C， WANG Y，et al.Tissue-specific expression and analysis of exogenous hormone response of BpPIN5 gene promoter in Betula platyphylla ［J］.Bulletin of Botanical Research，2022，42（1）：104-111.
52	王博，曹红利，黄玉婷，等.茶树生长素外运载体基因CsPIN3的克隆与表达分析［J］.作物学报，2016，42（1）：58-69.
52	WANG B， CAO H L， HUANG Y T，et al.Cloning and expression analysis of auxin efflux carrier gene CsPIN3 in tea plant（Camellia sinensis）［J］.Acta Agronomica Sinica，2016，42（1）：58-69.
53	李玉军，赵燕，黄丽华，等.十字花科植物PIN3基因调控元件及表达分析［J］.中国农业大学学报，2017，22（7）：24-33.
53	LI Y J， ZHAO Y， HUANG L H，et al.Regulating elements and expression analysis of PIN3 gene in Brassicaceae family［J］.Journal of China Agricultural University，2017，22（7）：24-33.
54	SEO P J， PARK C M.Auxin homeostasis during lateral root development under drought condition［J］.Plant Signaling & Behavior，2009，4（10）：1002-1004.
55	WANG W， DING G D， WHITE P J，et al.Mapping and cloning of quantitative trait loci for phosphorus efficiency in crops：opportunities and challenges［J］.Plant and Soil，2019，439（1/2）：91-112.
56	ADAMOWSKI M， FRIML J.PIN-dependent auxin transport：action，regulation，and evolution［J］.The Plant Cell，2015，27（1）：20-32.
57	ZHANG X， LIU L， WANG H，et al. MtPIN1 and MtPIN3 play dual roles in regulation of shade avoidance response under different environments in Medicago truncatula ［J］.International Journal of Molecular Sciences，2020，21（22）：8742.

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