Effect of Carbon Source, Auxin and Elicitor on the Growth and Synthesis of Secondary Metabolites of Adventitious Roots of Cajanus cajan(Linn.) Millsp.

doi:10.7525/j.issn.1673-5102.2018.03.011

Abstract

Abstract: Using 1/2MS as basic medium, we studied sucrose, IBA, jasmonic acid methylester(MJ) and salicylic acid(SA) on the influence of biomass and synthesis of secondary metabolites of adventitious root of Cajanus cajan(Linn.) Millsp.. The content of sucrose had a significant influence on the biomass and synthesis of secondary metabolites. When the content of sucrose was 30 g·L^-1, the amount of biomass and the content of secondary metabolites of adventitious root of C. cajan(Linn.) Millsp were the maximum value. The low concentration of IBA is beneficial to the growth of adventitious root of C. cajan(Linn.) Millsp. and the accumulation of secondary metabolites. However, the high content of IBA shows inhibition. When the content of IBA was 0.1 mg·L^-1, biomass, genistein and apigenin were maximum, 1.1, 1.1 and 2.8 times as control group respectively. MJ with 0-200 μmol·L^-1 almost had no effect on adventitious roots growth(P>0.05), but it had a significant effect on the synthesis of secondary metabolites. When the content of MJ was 100 μmol·L^-1, genistein and apigenin were maximum, respectively, 1.9 and 2.1 times as the control group. The growth of adventitious roots and the synthesis of genistein were inhibited by SA. However, SA had a certain effect on the synthesis of apigenin. When the content of SA was 100 μmol·L^-1, the content of apigenin was maximum, 1.5 times as the control group. Suspension culture of adventitious roots of C. cajan(Linn.) was an effective method to obtain the secondary metabolites, and will provide a good way of thinking for large-scale production of genistein and apigenin.

Key words: Cajanus cajan(Linn. ), adventitious roots, biomass, secondary metabolites

CLC Number:

Q949.751.9

FAN Yan-Min, DANG Shi-Kun, WANG Wen-Jie, WANG Hui-Mei. Effect of Carbon Source, Auxin and Elicitor on the Growth and Synthesis of Secondary Metabolites of Adventitious Roots of Cajanus cajan(Linn.) Millsp.[J]. Bulletin of Botanical Research, 2018, 38(3): 391-398.

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