Cloning and Expression Analysis of a Transcription Factor SlYABBY5 in Solanum lycopersicum

doi:10.7525/j.issn.1673-5102.2015.02.012

Abstract

Abstract: YABBY family is a small plant-specific gene family, with a typically N-terminal C2C2-type zinc finger domain and the C-terminal helix-loop-helix YABBY domain. By homologous sequence analysis, a YABBY transcription factor was cloned from tomato (Solanum lycopersicon), and named SlYABBY5(GenBank accession number: AK246138). By bioinformatics analysis, the length of SlYABBY5 ORF is 549 bp, encoding 182 amino acid residues with relative molecular weight of 20.58 kD and an isoelectric point of 8.78. By real-time PCR analysis, SlYABBY5 gene might be involved in the development of tomato fruits and leaves. Expression of SlYABBY5 down-regulated under the treatments of IAA, ABA, GA₃, ASA and low temperature stress, while up-regulated under salt stress and leaves wounding. Our results suggest that SlYABBY5 might participate in the growth and development of tomato and abiotic stress responses, and provide the basis for unrevealing the function of SlYABBY5 gene in tomato growth and development as well as stress signaling pathway.

Key words: YABBY family, SlYABBY5 gene, expression pattern analysis, abiotic stress

CLC Number:

WANG Su-Su1；CHEN Guo-Ping1；ZHANG Yan-Jie1；YIN Wen-Cheng1；ZHU Ming-Ku1； ZHANG Jian-Ling1；TANG Qu-Lin2；HU Zong-Li1*. Cloning and Expression Analysis of a Transcription Factor SlYABBY5 in Solanum lycopersicum[J]. Bulletin of Botanical Research, 2015, 35(2): 240-249.

References

1.Bowman J L.The YABBY gene family and abaxial cell fate［J］.Current Opinion in Plant Biology,2000,3(1):17-22.

2.Siegfried K R,Eshed Y,Baum S F,et al.Members of the YABBY gene family specify abaxial cell fate in Arabidopsis［J］.Development,1999,126(18):4117-4128.

3.Sawa S,Watanabe K,Goto K,et al.FILAMENTOUS FLOWER,a meristem and organ identity gene of Arabidopsis,encodes a protein with a zinc finger and HMG related domains［J］.Genes Dev,1999,13:1079-1088.

4.Baxevanis A D,Landsman D.The Hmg-1 Box Protein Family-Classification and Functional-Relationships［J］.Nucleic Acids Research,1995,23(9):1604-1613.

5.Bowman J L.The YABBY gene family and abaxial cell fate［J］.Current Opinion in Plant Biology,2000,3(1):17-22.

6.Siegfried K R,Eshed Y,Baum S F,et al.Members of the YABBY gene family specify abaxial cell fate in Arabidopsis［J］.Development,1999,126(18):4117-4128.

7.Stahle M I,Kuehlich J,Staron L,et al.YABBYs and the Transcriptional Corepressors LEUNIG and LEUNIG_HOMOLOG Maintain Leaf Polarity and Meristem Activity in Arabidopsis［J］.Plant Cell,2009,21(10):3105-3118.

8.Sarojam R,Sappl P G,Goldshmidt A,et al.Differentiating Arabidopsis Shoots from Leaves by Combined YABBY Activities［J］.Plant Cell,2010,22(7):2113-2130.

9.Bowman J L,Smyth D R.CRABS CLAW,a gene that regulates carpel and nectary development in Arabidopsis,encodes a novel protein with zinc finger and helix-loop-helix domains［J］.Development,1999,126:2387-2396.

10.Villanueva J M,Broadhvest J,Hauser B A,et al.INNER NO OUTER regulates abaxial-adaxial patterning in Arabidopsis ovules［J］.Genes Dev,1999,13:3160-3169.

11.Juarez M T,Twigg R W,Timmermans M C P.Specification of adaxial cell fate during maize leaf development［J］.Development,2004,131(18):4533-4544.

12.Yamaguchi T,Nagasawa N,Kawasaki S,et al.The YABBY gene DROOPING LEAF regulates carpel specification and midrib development in Oryza sativa［J］.Plant Cell,2004,16(2):500-509.

13.Dai M Q,Zhao Y,Ma Q F,et al.The rice YABBY1 gene is involved in the feedback regulation of gibberellin metabolism［J］.Plant Physiology,2007,144(1):121-133.

14.Dai M,Hu Y,Zhao Y,et al.A WUSCHEL-LIKE HOMEOBOX gene repressed a YABBY gene expression required for rice leaf development［J］.Plant Physiol,2007,144(1):380-390.

15.Liu H L,Xu Y Y,Xu Z H,et al.A rice YABBY gene,OsYABBY4,preferentially expresses in developing vascular tissue［J］.Dev Genes Evol,2007,217(9):629-637.

16.Zhao W,Su H Y,Song J,et al.Ectopic expression of TaYAB1,a member of YABBY gene family in wheat,causes the partial abaxialization of the adaxial epidermises of leaves and arrests the development of shoot apical meristem in Arabidopsis［J］.Plant Sci,2006,170(2):364-371.

17.Toriba T,Harada K,Takamura A,et al.Molecular characterization the YABBY gene family in Oryza sativa and expression analysis of OsYABBY1［J］.Molecular Genetics and Genomics,2007,277(5):457-468.

18.Takahiro Yamaguchi,Nobuhiro Nagasawa,Shinji Kawasaki,et al.The YABBY Gene DROOPING LEAF Regulates Carpel Specification and Midrib Development in Oryza sativa［J］.The Plant Cell,2004,16,500-509.

19.Tanaka W,Toriba T,Ohmori Y,et al.The YABBY gene TONGARI-BOUSHI1 is involved in lateral organ development and maintenance of meristem organization in the rice spikelet［J］.Plant Cell ,2012,24(1):80-95.

20.Huang Z J,Van Houten J,Gonzalez G,et al.Genome-wide identification,phylogeny and expression analysis of SUN,OFP and YABBY gene family in tomato［J］.Molecular Genetics and Genomics,2013,288(3-4):111-129.

21.Cong B,Barrero L S,Tanksley S D.Regulatory change in YABBY-like transcription factor led to evolution of extreme fruit size during tomato domestication［J］.Nature Genetics,2008,40(6):800-804.

22.Bartley G E,Ishida B K.Digital fruit ripening:Data mining in the TIGR tomato gene index［J］.Plant Molecular Biology Reporter,2002,20(2):115-130.

23.Bartley G E,Ishida B K.Developmental gene regulation during tomato fruit ripening and in-vitro sepal morphogenesis［J］.BMC Plant Biology,2003,3:4.

24.Bartley G E,Ishida B K.Ethylene-sensitive and insensitive regulation of transcription factor expression during in vitro tomato sepal ripening［J］.Journal of Experimental Botany,2007,58(8):2043-2051.

25.Minsung Kim,Thinh Pham1,Ashley Hamidi,et al.Reduced leaf complexity in tomato wiry mutants suggests a role for PHAN and KNOX genes in generating compound leaves［J］.Development,2003,130,4405-4415.

26.Vrebalov J,Ruezinsky D,Padmanabhan V,et al.A MADS-box gene necessary for fruit ripening at the tomato ripening-inhibitor(rin) locus［J］.Science,2002,296(5566):343-346.

27.Wilkinson J Q,Lanahan M B,Yen H C,et al.An Ethylene-Inducible Component of Signal-Transduction Encoded by Never-Ripe［J］.Science,1995,270(5243):1807-1809.

28.Vrebalov J,Pan I L,Arroyo A J M,et al.Fleshy Fruit Expansion and Ripening Are Regulated by the Tomato SHATTERPROOF Gene TAGL1［J］.Plant Cell,2009,21(10):3041-3062.

29.张建苓，陈国平，朱明库，等.番茄DEAD-box RNA解旋酶基因SlDEAH1的克隆及表达分析［J］.植物研究，2014，34(1):44-52.

30.吴杨，贺俐，李伟，等.斑茅δ-OAT基因克隆及序列分析［J］.植物研究，2009，29（5）:577-584.

31.Schneider M,Tognolli M,Bbiroch A.The Swiss-Protprotein knowledgebase and expasy:providing the plant community with high quality proteomic data and tools［J］.Plant Physiol Biochem,2004(42):1013-1021.

32.Nakai K,Horton P.PSORT：a program for detecting sorting signals in proteins and predicting their suncelluar localization［J］.Trends Biochem Sci,1999(24):34-36.

33.刘超,张林华,邱红林,等.天山雪莲sikGLP基因的克隆及表达分析［J］.植物研究,2013,33(4):461-467.

34.Exposito-Rodriguez M,Borges A A,Borges-Perez A,et al.Selection of internal control genes for quantitative real-time RT-PCR studies during tomato development process［J］.Bmc Plant Biology,2008,8(1):131.

35.Hsieh T H,Li C W,Su R C,et al.A tomato bZIP transcription factor,SlAREB,is involved in water deficit and salt stress response［J］.Planta,2010,231(6):1459-1473.

36.Yang M G,Yang Q Y,Fu T D,et al.Overexpression of the Brassica napus BnLAS gene in Arabidopsis affects plant development and increases drought tolerance［J］.Plant Cell Reports,2011,30(3):373-388.

37.Exposito-Rodriguez M,Borges A A,Borges-Perez A,et al.Selection of internal control genes for quantitative real-time RT-PCR studies during tomato development process［J］.Bmc Plant Biology,2008,8(1):131.

38.Hsieh T H,Li C W,Su R C,et al.A tomato bZIP transcription factor,SlAREB,is involved in water deficit and salt stress response［J］.Planta,2010,231(6):1459-1473.

39.郭书巧,黄骥,江燕,等.水稻C2H2型锌指蛋白基因RZF71的克隆与表达分析［J］.遗传，2007,29（5）：607-613.

40.莫晓婷,赵军,范云六,等.玉米转录因子结构与功能研究进展［J］.中国农业科技导报,2013,15(3):7-17.

41.Jibran R,Hunter D A,Dijkwel P P.Hormonal regulation of leaf senescence through integration of developmental and stress signals［J］.Plant Molecular Biology,2013,82(6):547-561.

42.李俊，傅兆麟.外源乙酰水杨酸对小麦灌浆期热害缓解效应的研究［J］.扬州大学学报,2013,34(1):56-59.

43.Gepstein S,Thimann K V.Changes in the Abscisic-Acid Content of Oat Leaves during Senescence［J］.Proceedings of the National Academy of Sciences of the United States of America-Biological Sciences,1980,77(4):2050-2053.

44.Yang J C,Zhang J H,Wang Z Q,et al.Abscisic acid and cytokinins in the root exudates and leaves and their relationship to senescence and remobilization of carbon reserves in rice subjected to water stress during grain filling［J］.Planta,2002,215(4):645-652.

45.Xue-Xuan X,Hong-Bo S,Yuan-Yuan M,et al.Biotechnological implications from abscisic acid (ABA) roles in cold stress and leaf senescence as an important signal for improving plant sustainable survival under abiotic-stressed conditions［J］.Critical Reviews in Biotechnology,2010,30(3):222-230.

[1]	Chunyao WANG, Xiaojin LEI, Zhongyuan LIU. Expression Pattern Analysis of PdbHMGs Genes in Populus davidiana×P. bolleana under Abiotic Stress [J]. Bulletin of Botanical Research, 2023, 43(6): 932-942.
[2]	Luhua ZHOU, Junyi FANG, Zimo XIONG, Weifeng WU, Jiarui LIU, Qiao LU, Hongqing LING, Danyu KONG. Evaluation on Waterlogging Tolerance of Different Tomato Germplasm [J]. Bulletin of Botanical Research, 2023, 43(5): 657-666.
[3]	Chaoran SHAN, Xiaohu CHEN, Yunfei DING, Wei ZHAO, Han LU, Shangzhu GAO, Fenghui QI, Yaguang ZHAN, Fansuo ZENG. Functional Analysis of FmCCoAOMT Gene in Fraxinus mandshurica During Lignin Synthesis and Abiotic Stress [J]. Bulletin of Botanical Research, 2023, 43(5): 768-778.
[4]	Bi QIN, Xiaoxiao WANG, Yushuang YANG, Qiuhai NIE, Qiuhui CHEN, Shizhong LIU. Identification and Expression Pattern Analysis of TkAPC10 in Taraxacum kok-saghyz Rodin [J]. Bulletin of Botanical Research, 2022, 42(5): 830-839.
[5]	Xueying WANG, Ruiqi WANG, Yang ZHANG, Cong LIU, Dean XIA, Zhigang WEI. Genome‑wide Identification and Stress Response Analysis of Cyclic Nucleotide-gated Channels(CNGC) Gene Family in Populus trichocarpa [J]. Bulletin of Botanical Research, 2022, 42(4): 613-625.
[6]	Qian Sun, Yuhang Wu, Yaxuan Zhang, Jingdan Cao, Jingjing Shi, Chao Wang. Bioinformatic Analysis and Expression Pattern of LTP Family Genes in Populus davidiana × P. alba var. pyramidalis [J]. Bulletin of Botanical Research, 2022, 42(2): 211-223.
[7]	Shuang Ma, Boya Wang, Ying Cao, Shanglian Hu, Zhimin Gao. Identification and Expression Analysis of Expansin Genes in Moso Bamboo (Phyllostachys edulis) [J]. Bulletin of Botanical Research, 2022, 42(1): 29-38.
[8]	Bo-Chao ZHANG, Jia-Lin WANG, Yuan YIN, Yi-Da CHE, Jun-Jie DENG, Rong-Shu ZHANG. Tissue Expression Patterns of PdPapWRKY51 in Shanxin Poplar (Populus davidiana × P. alba var. pyramidlis) under Stress Conditions [J]. Bulletin of Botanical Research, 2021, 41(6): 911-920.
[9]	Xiao-Xiao WANG, Bi QIN, Yu-Shuang YANG, Qiu-Hai NIE, Ji-Chuan ZHANG, Shi-Zhong LIU. Cloning and Expression Analysis of E2 Ubiquitin-conjugating Enzyme Gene TkUBC2 in Taraxacum kok-saghyz Rodin [J]. Bulletin of Botanical Research, 2021, 41(1): 98-106.
[10]	JIANG Cheng, ZHANG Xi, TIAN Qing, LI Li. Isolation of the BpbHLH112 Gene and Expression Analysis of Its Promoter in Betula platyphylla [J]. Bulletin of Botanical Research, 2020, 40(4): 583-592.
[11]	MA Miao-Miao, LI Cheng-Hao, LIU Xiao, ZHANG Xin, YANG Jing-Li. Cloning and Bioinformatics Analysis of LoERF017 Gene from Larix kaempferi [J]. Bulletin of Botanical Research, 2020, 40(4): 602-612.
[12]	ZHANG Yu-Qing, LIU Ye, QU Chun-Pu, LIU Guan-Jun, YANG Tian-Tian, YANG Cheng-Jun. Resistance Analysis of Transgenic PnDof30 Arabidopsis under Abiotic Stress [J]. Bulletin of Botanical Research, 2020, 40(3): 407-415.
[13]	LIU Jia-Li, HE Ming-Liang, LIU Chen-Xi, LIAO Xu, LI Xiu-Feng, GUAN Qing-Jie. Zinc Finger Protein OsZFP6 Expression Features and Functions in Saline-alkali Stress Response [J]. Bulletin of Botanical Research, 2020, 40(3): 424-432.
[14]	LI Ya-Bo, Lü Jia-Xin, TAN Bing, GAO Cai-Qiu. Cloning and expression analysis of 5 ZFP genes from Poplus trichocarpa [J]. Bulletin of Botanical Research, 2020, 40(2): 243-250.
[15]	QIN Lin-Lin, ZHANG Xi, JIANG Cheng, LI Li. Cloning and Functional Analysis of BpZFP4 Promoter from Birch(Betula platyphylla) [J]. Bulletin of Botanical Research, 2019, 39(6): 917-926.