Research on ISSR Identification Technology of Larch Species and Clones

doi:10.7525/j.issn.1673-5102.2008.02.018

Bulletin of Botanical Research ›› 2008, Vol. 28 ›› Issue (2): 216-221.doi: 10.7525/j.issn.1673-5102.2008.02.018

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Research on ISSR Identification Technology of Larch Species and Clones

ZHANG Lei;ZHANG Han-Guo*;LI Xue-Feng;JIANG Jing;WEI Zhi-Gang

(Key Laboratory of Forest Tree Genetic Improvement and Biotechnology of Ministry of Education,Northeast Forestry University,Harbin　150040)

Received:1900-01-01 Revised:1900-01-01 Online:2008-03-20 Published:2008-03-20
Contact: ZHANG Han-Guo
Supported by:

Abstract

Abstract: This study used ISSR molecular marker technology to identify Larix gmelinii, Larix olgensis, Larix kaempferi and different clones. Thirteen ISSR primers which screened from fortynine primers can identify among larch species and clones，and the rate of the samples with specific band is 100%, this technology offers new ways and means for accurate identification of new larch species and elite seeds: there are five primers by which L. kaempferi, L. olgensis and L. kaempferi were amplified particularly, specific bands were produced at different location, as a species identification standards, nine primers can be amplified specific fragment in different kinds of larch clones respectively. It can identify the clones.

Key words: larch, species, clone, ISSR, identify

CLC Number:

ZHANG Lei;ZHANG Han-Guo*;LI Xue-Feng;JIANG Jing;WEI Zhi-Gang. Research on ISSR Identification Technology of Larch Species and Clones[J]. Bulletin of Botanical Research, 2008, 28(2): 216-221.

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Research on ISSR Identification Technology of Larch Species and Clones

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