The Effects of Mixed Eucalyptus Plantations with Different Mycorrhizal Tree Species on Soil Microbial Community

doi:10.7525/j.issn.1673-5102.2025.03.014

Abstract

Abstract:

Mixed-species plantations can promote soil nutrient cycling， productivity， and ecosystem functions. Mycorrhizal associations play important roles in affecting species coexistence and nutrient cycling in mixed plantations. However， our understanding on the effects of mixed tree species with different mycorrhizal types on soil microbial community associated with soil nutrients of Eucalyptus plantations remains limited. In this study， we conducted metagenomic sequencing to investigate the changes in soil microbial community structure and functions， co-occurrence networks between bacteria and fungi， and their relationships with soil nutrients for one monospecific Eucalyptus plantation and three mixed Eucalyptus plantations with different mycorrhizal tree species， including Dalbergia odorifera（arbuscular mycorrhizal and N₂-fixing， AM-NF）， Michelia macclurei（arbuscular mycorrhizal， AM）， and Quercus acutissima（ectomycorrhizal， ECM）. The results indicated that the mixed stands significantly increased the richness of soil microbial community， enhanced the abundance of genes related to soil nutrient cycling， and increased the complexity of microbial co-occurrence networks. The mixed plantations of Eucalyptus with D. odorifera had the highest soil microbial richness， relative abundance of bacterial community associated with aerobic chemoheterotrophy and fermentation， and the modularity of fungal-bacterial co-occurrence network； meanwhile， either the bacterial or fungal communities were significantly different from the other three plantations. The mixed Eucalyptus plantations with M. macclurei or Q. acutissima species had the highest abundance of microbial functional genes such as hydrocarbon degradation， nitrogen fixation， and methanotrophy. In addition， the microbial co-occurrence networks in the mixed Eucalyptus plantations with Q. acutissima have the highest number of edges， average degree， and network stability. The richness of key microbes in co-occurrence networks was closely correlated with soil $N O 3 -$ level. However， the richness of microbial community of the networks in the mixed Eucalyptus plantations with D. odorifera and M. macclurei was largely associated with soil $N H 4 +$ level.

Key words: Eucalyptus, mixed-species plantations, mycorrhizal type, soil microbial community, co-occurrence network

CLC Number:

Q939.96

Yaxin WANG, Yuan ZHU, Sen MENG, Angang MING, Hongyan JIA, Fangcuo QIN, Junkun LU. The Effects of Mixed Eucalyptus Plantations with Different Mycorrhizal Tree Species on Soil Microbial Community[J]. Bulletin of Botanical Research, 2025, 45(3): 447-459.

Figures/Tables 10

Fig.1

Fig.2

Table 1

Soil physical and chemical properties in monospecific Eucalyptus plantation and three mixed Eucalyptus plantations

林型 Plantation type	pH	铵态氮 $N H 4 +$ -N/（mg∙kg^-1）	硝态氮 $N O 3 -$ -N/（mg∙kg^-1）	全氮 TN/（g∙kg^-1）	有机碳 SOC/（mg∙kg^-1）	全磷 TP/（g∙kg^-1）	全钾 TK/（g∙kg^-1）	有效磷 AP/（g∙kg^-1）	有效钾 AK/（g∙kg^-1）
EU	4.41±0.20^ab	2.78±1.20^a	12.20±2.34^ab	1.19±0.36^a	24.95±4.92^a	0.25±0.04^a	9.16±3.57^a	2.42±0.71^a	62.97±2.98^b
EU+DO	4.37±0.17^ab	2.88±1.20^a	13.82±2.54^a	1.18±0.34^a	20.58±2.48^b	0.25±0.03^ab	9.39±2.97^a	2.22±0.51^a	60.67±3.08^b
EU+MM	4.51±0.27^a	1.28±0.78^b	14.32±2.69^a	1.16±0.10^a	22.96±1.23^ab	0.22±0.03^b	8.34±1.42^ab	1.81±0.68^ab	71.82±4.49^a
EU+QA	4.48±0.19^ab	2.16±0.19^ab	13.37±1.82^ab	0.98±0.14^ab	23.56±2.10^a	0.22±0.02^ab	7.95±1.28^abc	1.98±0.41^ab	52.10±1.82^c

Table 1

Fig.3

Table 2

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Fig.5

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林型 Plantation type	平均最短路径 Average shortest path length	平均度 Average degree	网络直径 Network diameter	网络密度 Network density	聚类系数 Clustering coefficient	模块化 Modularity	正相关关系 Positive correlation	负相关关系 Negative correlation	稳定性 Stability
EU	4.705	8.534	13.367	0.028	0.595	0.577	1 247	80	0.545
EU+DO	3.497	4.986	12.484	0.036	0.564	0.684	333	16	0.039
EU+MM	3.505	17.383	14.883	0.093	0.714	0.311	1 317	317	0.133
EU+QA	18.200	30.521	9.167	0.163	0.925	0.153	2 687	182	0.882

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