Evaluation of Antioxidant, Anti-ultraviolet Radiation, and Antibacterial Functions of Endophytic Extracts from Different Plant Sources

doi:10.7525/j.issn.1673-5102.2025.03.013

Abstract

Abstract:

This study evaluated the antioxidant， antimicrobial， UV radiation resistance properties， and secondary metabolite content of fermented extracts from 20 endophytic fungi of diverse plant origins to assess their potential in cosmetic applications. ABTS and DPPH radical scavenging rates of fermentation broths and mycelial extracts were measured using microplate reader colorimetry， while ferric ion reduction capacity was determined via FRAP assay. Antimicrobial activity was assessed using the Oxford cup method. The results indicated strong antioxidant activity in fermentation broths of strains XY1， YZ6， ZJ29， XG4-2， and ZBS49. ZJ29 exhibited the highest DPPH radical scavenging rate （93.62%）， while XY1 showed the highest ABTS radical scavenging rate （90.47%）. ZJ29 fermentation broth also demonstrated an ABTS radical scavenging rate >50% and the highest FRAP value （2 974.55 μmol⋅L^-¹）. Tyrosinase inhibition by ZJ29 and ZL18-1 mycelial extracts reached 60.62% and 98.10%， respectively， with ZJ29 fermentation broth showing the strongest inhibition（69.78%）. ZJ29 fermentation broth exhibited the highest inhibition against Staphylococcus aureus. The UVA and UVB absorbance of YZ6 and ZJ29 was higher than 2.8， and the UVA absorbance of YZ6 and ZJ29 was higher than 2.0 and 1.5， respectively. YZ6 mycelial extract also showed strong resistance to UVB and UVA， with absorbance of 1.5-2.5. YH3 and XY1 mycelial extracts contained the higher polyphenol （18.47 and 15.36 mg⋅g^-¹） and flavonoid （4.35 and 7.15 mg⋅g^-¹） content， respectively. ZL23 and ZJ29 had the higher triterpene content （16.79 and 11.20 mg⋅g^-¹， respectively）. In conclusion， ZJ29 mycelial extracts had superior antimicrobial， UV radiation resistance， and antioxidant activities， followed by YZ6 and XY1， highlighting their significant potential for daily chemical products.

Key words: endophytic fungi, secondary metabolites, antioxidation, anti-ultraviolet radiation, bacteriostasis

CLC Number:

Q939.5

Kunlin ZOU, Siyuan JIANG, Yajing WEI, Shun ZHANG, Peng LI, Jing YIN. Evaluation of Antioxidant, Anti-ultraviolet Radiation, and Antibacterial Functions of Endophytic Extracts from Different Plant Sources[J]. Bulletin of Botanical Research, 2025, 45(3): 433-446.

Figures/Tables 10

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References 44

1	吴琼，王雷，曹涤非，等.汉麻内生菌的研究进展［J］.农业与技术，2022，42（5）：25-27.
	WU Q， WANG L， CAO D F，et al.Research progress of endophytic bacteria of Chinese hemp［J］.Agriculture and Technology，2022，42（5）：25-27.
2	胡倩楠.荒漠植物深色有隔内生真菌物种多样性及耐旱性研究［D］.保定：河北大学，2023.
	HU Q N.Species diversity and drought tolerance of dark septate endophytes fungi in desert plants［D］.Baoding：Hebei University，2023.
3	甘俊丽.黄草乌内生真菌黑曲霉（Aspergillus niger B-30）次生代谢产物及活性研究［D］.昆明：云南大学，2022.
	GAN J L.Secondary metabolites analysis and their bioactivities of the endophytes fungus（Aspergillus niger B-30） associated with Aconitum vilmorinianum Kom［D］.Kunming：Yunnan University，2022.
4	杨星.山豆根内生真菌TRXY-46抑菌次生代谢产物研究［D］.南宁：广西大学，2020.
	YANG X.Research on the antagonistic phytopathogen secondary metabolites of endophytic fungi TRXY-46 from Sophora tonkinensis ［D］.Nanning：Guangxi University，2020.
5	郑有坤，刘凯，熊子君，等.药用植物内生放线菌多样性及天然活性物质研究进展［J］.中草药，2014，45（14）：2089-2099.
	ZHENG Y K， LIU K， XIONG Z J，et al.Research progress on biodiversity of endophytic actinobacteria in medicinal plants and their bioactive substances［J］.Chinese Traditional and Herbal Drugs，2014，45（14）：2089-2099.
6	ZHANG H W， SONG Y C， TAN R X.Biology and chemistry of endophytes［J］.Natural Product Reports，2006， 23（5）：753-771.
7	卜宣尹，杨卫丽.植物内生菌抑菌机制和抑菌次生代谢产物的研究进展［J］.现代药物与临床，2021，36（10）：2200-2206.
	BU X Y， YANG W L.Research progress on antibacterial mechanism of plant endophytes and antibacterial secondary metabolites［J］.Drugs & Clinic，2021，36（10）：2200-2206.
8	FISHER P J， ANSON A E， PETRINI O.Fungal endophytes in Ulex europaeus and Ulex gallii ［J］.Transactions of the British Mycological Society，1986，86（1）：153-156.
9	陈洁梅，缪冰旋，张灿辉，等.野生葡萄及葡萄酒抗氧化活性和抗菌性研究［J］.广西植物，2014，34（4）：510-514.
	CHEN J M， MIAO B X， ZHANG C H，et al.Antioxidant activity and antibacterial property of wild grape and grape wine［J］.Guihaia，2014，34（4）：510-514.
10	LU H， ZOU W X， MENG J C，et al.New bioactive metabolites produced by Colletotrichum sp.，an endophytic fungus in Artemisia annua ［J］.Plant Science，2000，151（1）：67-73.
11	杨雨微，周向平，袁志辉，等.银杏内生菌及其次生代谢产物多样性和生防活性的研究进展［J］.安徽农业科学，2024，52（22）：20-28.
	YANG Y W， ZHOU X P， YUAN Z H，et al.Endophytes of Ginkgo biloba and its secondary metabolites diversity and biocontrol activity［J］.Journal of Anhui Agricultural Sciences，2024，52（22）：20-28.
12	苏天骄.川贝母基源植物内生真菌及其次生代谢物生物活性研究［D］.雅安：四川农业大学，2018.
	SU T J.Biological activities of endophytic fungi and their metabolites from the original plants of Fritillariae Cirrhosae Bulbus［D］.Ya’an：Sichuan Agricultural University，2018.
13	MECHOULAM R， GAONI Y.Recent advances in the chemistry of hashish［J］.Fortschritte der Chemie Organischer Naturstoffe，1967，25：175-213.
14	张政帅，周学明，田欣，等.角果木来源内生真菌 Penicillium herquei JX4中聚酮类次级代谢产物的抗菌性［J］.海南师范大学学报（自然科学版），2025，38（1）：10-13.
	ZHANG Z S， ZHOU X M， TIAN X，et al.Antibacterial activity of secondary metabolites of polyketones from Ceriops tagal-derived endophytic fungus Penicillium herquei JX4［J］.Journal of Hainan Normal University （Natural Science），2025，38（1）：10-13.
15	王静，赵浩军，刘梦龙，等.地茶属地衣内生真菌次级代谢产物抑菌、抗氧化活性研究［J］.中成药，2024， 46（7）：2429-2433.
	WANG J， ZHAO H J， LIU M L，et al.Study on the inhibitory and antioxidant activities of secondary metabolites of endophytic fungi in lichen of Diecantha genus ［J］.Chinese Traditional Patent Medicine，2024，46（7）：2429-2433.
16	尹诗雨，郭砚.天然植物提取物对皮肤光老化的保护作用［J］.临床医学进展，2022，12（1）：726-731．
	YIN S Y， GUO Y.Protective effect of natural plant extracts on skin photoaging［J］.Advances in Clinical Medicine，2022，12（1）：726-731.
17	曹森，王婷，高天，等.叶底珠内生真菌提取物的抗炎镇痛和抗氧化活性研究［J］.药物评价研究，2021， 44（6）：1232-1239.
	CAO S， WANG T， GAO T，et al.Study on anti-inflammatory，analgesic and antioxidative activities of extracts from Securinega suffruticosa ［J］.Drug Evaluation Research，2021，44（6）：1232-1239.
18	吴凯宾.抗氧化在化妆品行业的应用进展［J］.化工设计通讯，2019，45（6）：143-146.
	WU K B.Research on the application progress of antioxidant in cosmetics industry［J］.Chemical Engineering Design Communications，2019，45（6）：143-146.
19	许先猛，董文宾，卢军，等.蒲公英的化学成分和功能特性的研究进展［J］.食品安全质量检测学报，2018（7）：1623-1627.
	XU X M， DONG W B， LU J，et al.Research progress on the chemical compositions and functional properties of dandelion［J］.Journal of Food Safety and Quality，2018（7）：1623-1627.
20	张歌珂.植物源舒缓活性物质的筛选及其活性研究［D］.无锡：江南大学，2023.
	ZHANG G K.Screening and activity study of soothing substances from plants［D］.Wuxi：Jiangnan University，2023.
21	吴以禄，钟怡莹，陈忻，等.山竹果皮在化妆品领域应用前景研究［J］.广东化工，2022，49（19）：129-131.
	WU Y L， ZHONG Y Y， CHEN X，et al.The application of mangosteen peel in cosmetics field［J］.Guangdong Chemical Industry，2022，49（19）：129-131.
22	黄振帝，苗华彪，裴丁玉，等.松口蘑的活性成分及其研究进展［J］.菌物学报，2023，42（10）：2025-2040.
	HUANG Z D， MIAO H B， PEI D Y，et al.Active components and their research progress of Tricholoma matsutake ［J］.Mycosystema，2023，42（10）：2025-2040.
23	MWANGI J K.The impact of rice blast disease，its mapping and suitability analysis for rice growing sites in the greater Mwea Region［D］.Nairobi：Jomo Kenyatta University of Agriculture and Technology，2014.
24	THAIPONG K， BOONPRAKOB U， CROSBY K，et al.Comparison of ABTS，DPPH，FRAP，and ORAC assays for estimating antioxidant activity from guava fruit extracts［J］.Journal of Food Composition and Analysis，2006，19（6/7）：669-675.
25	杜小豪，徐卫.防晒化妆品防晒效果评价方法——紫外吸光度法的研究［J］.日用化学工业，1998（3）：43-46.
	DU X H， XU W.The method of sunscreen effect evaluation of cosmetics：ultra-violet spectroscopy［J］.China Surfactant Detergent & Cosmetics，1998（3）：43-46.
26	李祥荣.海洋真菌抗副溶血弧菌活性菌株的筛选及其活性物质的初步研究［D］.连云港：江苏海洋大学，2022.
	LI X R.Screening of marine fungi active strains against Vibrio parahaemolyticus and preliminary study on their active substances［D］.Lianyungang：Jiangsu Ocean University，2022.
27	柳成东，赵平，王乙茹，等.凝结芽孢杆菌抑菌性能的研究进展［J］.饲料工业，2021，42（10）：58-64.
	LIU C D， ZHAO P， WANG Y R，et al.Research progress on antibacterial properties of a probiotic Bacillus coagulans ［J］.Feed Industry，2021，42（10）：58-64.
28	周曼，曹唯仪，王玉蓉.葡萄籽中多酚类物质提取工艺的研究［J］.中医药信息，2012，29（1）：53-55.
	ZHOU M， CAO W Y， WANG Y R.The extraction process of polyphenols from grape seed［J］.Information on Traditional Chinese Medicine，2012，29（1）：53-55.
29	陶海腾，陈晓明，吕飞杰，等.木薯叶黄酮类化合物提取研究［J］.食品研究与开发，2009，30（12）：12-15.
	TAO H T， CHEN X M， LÜ F J，et al.Study on the extraction of flavonoids from cassava leaves［J］.Food Research and Development，2009，30（12）：12-15.
30	李春晓，尹静，詹亚光，等.水分、氮肥及MeJA处理对白桦三萜积累特性的影响［J］.西北植物学报，2012，32（1）：155-161.
	LI C X， YIN J， ZHAN Y G，et al.Effects of water，nitrogen and methyl jasmonate treatment on triterpenes accumulation in birch （Betula platyphylla Suk.）［J］.Acta Botanica Boreali-Occidentalia Sinica，2012，32（1）：155-161.
31	黄颖，孟慧，何欣，等.沉香属植物内生真菌多样性及功能研究进展［J］.中草药，2024，55（4）：1397-1406.
	HUANG Y， MENG H， HE X，et al.Research progress on diversity and functions of endophytic fungi in Aquilaria genus［J］.Chinese Traditional and Herbal Drugs，2024，55（4）：1397-1406.
32	祁鹤兴，李红芳，邹海涛，等.青贮玉米来源镰刀菌的分离鉴定及其致病性分析［J/OL］.分子植物育种，（2023-10-17）［2024-12-30］..
	QI H X， LI H F， ZOU H T，et al.Identification and pathogenicity analysis of Fusarium pathogens from silage maize［J/OL］.Molecular Plant Breeding，（2023-10-17）［2024-12-30］..
33	金哲雄，廖薇，张闯，等.引起山核桃干腐病的镰刀菌鉴定［J］.植物病理学报，2024，54（2）：443-446.
	JIN Z X， LIAO W， ZHANG C，et al.Identification of Fusarium oblongum causing trunk canker disease on Carya cathayensis ［J］.Acta Phytopathologica Sinica，2024， 54（2）：443-446.
34	韦凯，梁莉，王萌，等.芒果叶中芒果苷抗氧化及抗紫外能力的研究［J］.食品安全导刊，2024（32）：80-82.
	WEI K， LIANG L， WANG M，et al.Study on the antioxidant and UV resistance of mangiferin in mango leaves［J］.China Food Safety Magazine，2024（32）：80-82.
35	贾景辉.基于酪氨酸酶抑制活性的槐耳次级代谢产物研究［D］.沈阳：辽宁大学，2023.
	JIA J H.Studied on the bioactive secondary metabolites from Huai’er with tyrosinase inhibitory activity ［D］.Shenyang：Liaoning University，2023.
36	张雪，李雪峰，胡瑞峰，等.具有抑制酪氨酸酶活性乳酸菌的筛选及其成分分析［J］.食品科学，2023， 44（18）：193-199.
	ZHANG X， LI X F， HU R F，et al.Screening and compositional analysis of lactic acid bacteria with tyrosinase inhibitory activity［J］.Food Science，2023，44（18）：193-199.
37	徐彤，姚雪丹，王玉苗，等.盐水球菌Salinicoccus ventosaetal B2-3-5的代谢产物分析及其抑制酪氨酸酶活性的机制［J］.微生物学通报，2021，48（10）：3753-3764.
	XU T， YAO X D， WANG Y M，et al.Analysis of metabolites from Salinicoccus ventosaetal B2-3-5 and their inhibitory mechanism of tyrosinase activity［J］.Microbiology China，2021，48（10）：3753-3764.
38	龙赟而，潘瑞雪，赵一宁，等.一株海洋源皮特不动杆菌的分离鉴定及其全基因测序分析［J/OL］.微生物学通报，（2024-12-5）［2024-12-30］..
	LONG Y E， PAN R X， ZHAO Y N，et al.Isolation，identification，and whole genome sequencing of a marine-derived strain of Acinetobacter pittii ［J/OL］.Microbiology China，（2024-12-5）［2024-12-30］..
39	戴万生，杨丽，邱斌，等.DPPH法评价黄精及炮制品抗氧化活性方法的研究［J］.辽宁中医杂志，2024，51（5）：152-154.
	DAI W S， YANG L， QIU B，et al.Study on DPPH method for evaluating antioxidant activity of Huangjing（Rhizoma Polygonati） and its processed products［J］.Liaoning Journal of Traditional Chinese Medicine，2024，51（5）：152-154.
40	胡宇航，陈梁，罗翌元，等.三叶青茎部不同溶剂提取物的功能成分、抗氧化及酪氨酸酶抑制活性研究［J］.食品安全质量检测学报，2023，14（10）：173-181.
	HU Y H， CHEN L， LUO Y Y，et al.Functional components，antioxidant activity and tyrosinase inhibitory activity of extracts from different solvent extracts from stems of Tetrastigma hemsleyanum Diels et Gilg［J］.Journal of Food Safety and Quality，2023，14（10）：173-181.
41	王琪.罗汉果内生菌产胞外多糖菌株的筛选及其抗氧化与降糖活性研究［D］.桂林：广西师范大学，2021.
	WANG Q.Selection of exopolysaccharide-producing strains from endophytic bacteria in Siraitia grosvenorii and antioxidant and hypoglycemic activity of exopolysaccharide［D］.Guilin：Guangxi Normal University，2021.
42	姜中宝，黄宣雅，唐振兴，等.具有抑菌和抗氧化活性紫金牛内生菌的筛选鉴定［J］.杭州师范大学学报（自然科学版），2023，22（5）：497-504.
	JIANG Z B， HUANG X Y， TANG Z X，et al.Screening and identification of endophytes with antibacterial and antioxidant activities from Ardisia japonica ［J］.Journal of Hangzhou Normal University （Natural Science Edition），2023，22（5）：497-504.
43	宋勇，顾玉琴，李小玉，等.一株鱼腥草内生真菌的菌种鉴定及抑菌活性初探［J］.现代预防医学，2018， 45（11）：2052-2055.
	SONG Y， GU Y Q， LI X Y，et al.Identification and the antimicrobial activity of an endophytic fungus isolated from Houttuynia cordata Thunb［J］.Modern Preventive Medicine，2018，45（11）：2052-2055.
44	裴晓燕.红树莓内生真菌次级代谢产物的抑菌活性研究［D］.兰州：西北师范大学，2024.
	PEI X Y.Study on antibacterial activity of secondary metabolites from endophytic fungi of red raspberry［D］.Lanzhou：Northwest Normal University，2024.

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[12]	YANG Bei-Fen;JIN Ze-Xin;SHAO Hong;LI Jun-Min. Analysis of Secondary Metabolites Contents in the Different Organs of Heptacodium miconioides* [J]. Bulletin of Botanical Research, 2007, 27(2): 229-232.
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