Cloning and Expression Analysis of HD-Zip Transcription Factor PsnHB63 in Populus simonii × P. nigra

doi:10.7525/j.issn.1673-5102.2021.06.019

Abstract

Abstract:

The PsnHB63 gene was cloned from the leaves of Populus simonii × P.nigra， and by using bioinformatics analysis， the results showed that the full-length cDNA fragment was 717 bp in length， encoded 238 amino acids， and had an unstable hydrophilic protein with a conserved domain， belonged to Homeobox/HALZ family. The phylogenetic tree showed that PsnHB63 was closely related to Populus tomentosa， Populus alba and Populus. The PsnHB63 was localized in the nucleus by subcellular localization. Under salt and drought stresses， the expression of PsnHB63 was up-regulated. The results indicated that the HD-Zip transcription factor HB63 might respond to high salt and drought stresses.

Key words: Populus simonii × P.nigra, transcription factors, subcellular localization, stress response

CLC Number:

S792.116

Lian-Bin HAN, Qing GUO, Kai ZHAO, Ting-Bo JIANG, Bo-Ru ZHOU, Li LI. Cloning and Expression Analysis of HD-Zip Transcription Factor PsnHB63 in Populus simonii × P. nigra[J]. Bulletin of Botanical Research, 2021, 41(6): 1006-1014.

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