Abstract:

To obtain a high-efficiency method of vegetative propagation for Amomum villosum， we studied the effects of different concentrations of α-naphthalene acetic acid（NAA） added on three kinds of cuttings of tip of rhizome（TR）， rhizome and erect stem basal（ESB）. The results showed the budding rate and rooting rate of cuttings of TR， rhizome and ESB which were not treated by NAA were at a low level respectively. The budding rates of the three cuttings were 10.0%， 3.3% and 23.3% respectively， and the rooting rates were 23.3%， 10.0% and 3.3%， respectively. Compared with controls， living rates， budding rates and rooting rates of three kinds of cuttings were increased dramatically. In sequence of TR， rhizome and ESB， living rates of three kinds of cuttings were increased by 17.7%， 23.4% and 26.7% respectively， budding rates were increased by 3.3%， 50.3% and 53.3% respectively， and rooting rates were increased by 43.3%， 26.7% and 53.4% respectively. The growth indexes of roots regenerated from three kinds of cuttings which were treated by 200 mg·L^-1 NAA were improved obviously. Comparing with controls， the number， length and diameter of roots regenerated from TR were increased by 56.7%， 82.6% and 29.2% respectively， the length of roots regenerated from rhizome was increased by 203.5%， and the length and diameter of buds regenerated from ESB were increased by 25.2% and 35.2% respectively. There were two ways that buds regenerated from the cuttings of TR treated by NAA. One was the original bud of tip recovered growing， but the other was the new bud regenerated from cutting nodes. NAA affected the cuttings of tip of rhizome， rhizome and erect stem basal in survival rate. Survival rates of three cuttings treated by 200 mg·L^-1 NAA increased dramatically comparing with control. Besides division propagation， a new high-efficiency method for vegetative propagation of A.villosum was set up.

Key words: Amomum villosum Lour., tip, rhizome, erect stem basal, survival rate, living rate