Cloning and Sequence Analysis of Squalene Synthase(SQS) Gene from Dioscorea bulbifera L. Microtuber

doi:10.7525/j.issn.1673-5102.2021.02.012

Abstract

Abstract:

The core fragment of SQS gene was screened from the transcriptome database of Dioscorea bulbifera L. microtuber， the conservative fragment of SQS gene was obtained by RT-PCR， and the 3' and 5' terminal sequences of SQS gene were obtained by race technique， and the sequence was analyzed by bioinformatics. The SQS gene of D.bulbifera L. microtuber was 1 548 bp long and 415 bp amino acid sequence， with a theoretical molecular weight of 46 786.38 D and an isoelectric point of 5.97. SQS protein is a hydrophobic protein without signal peptide. It contains the functional domain necessary for SQS belonging to Isoprenoid_Biosym_C1 superfamily. The homology of SQS protein between D.bulbifera and other plants is high， and the amino acid similarity of SQS protein between D.bulbifera and D.zingiberensis is 96.4%. The full-length sequence of SQS gene was obtained for the first time from D.bulbifera L. microtuber， which has the typical characteristics of SQS homologous gene. It provides the basis for further study on SQS gene structure， gene expression and gene mutation of D.bulbifera microtuber， and provides data support for the correlation analysis of positive selection site and function of SQS， a key enzyme of triterpene synthesis pathway in Dioscorea.

Key words: Dioscorea bulbifera L., microtuber, squalene synthase（SQS）, gene cloning, sequence analysis

CLC Number:

S567.9

Wei-Zhong KE, Wen-Juan ZHONG, Kai-Ying LIU, Xiang-Yuan LI, Ming-Hua YIN. Cloning and Sequence Analysis of Squalene Synthase(SQS) Gene from Dioscorea bulbifera L. Microtuber[J]. Bulletin of Botanical Research, 2021, 41(2): 243-250.

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