Effects of Nitrogen Deposition and Ectomycorrhizal Fungi on Root Architecture and Rhizosphere Soil Enzyme Activities of Larix olgensis Seedlings

doi:10.7525/j.issn.1673-5102.2021.01.018

Abstract

Abstract:

In order to explore the responses of root morphology and rhizosphere soil enzyme activities of one-year-old Larix olgensis seedlings to different simulated nitrogen deposition and ectomycorrhizal fungi， the morphology of roots with diameter of 0-0.5 mm（including the total root length， surface area， volume and tips） and soil enzyme activities（including β-1，4-glucosidase（BG）， L-leucine aminopeptidase（LAP）， β-1，4-N-acetylglucosaminidase（NAG）， acid phosphatase（ACP） and alkaline phosphatase（ALP）） were measured respectively. The different inoculated seedlings of L.olgensis were selected， including the not noculated（-M） and inoculated with 8 ectomycorrhizal fungi（+M） respectively. Four nitrogen deposition treatments were set， including no nitrogen（0N， 0 kg·N·hm^-1·yr^-1）， low nitrogen（LN， 15 kg·N·hm^-1·yr^-1）， middle nitrogen（MN， 30 kg·N·hm^-1·yr^-1） and high nitrogen（HN， 60 kg·N·hm^-1·yr^-1）. The results indicated that： ①The nitrogen deposition concentration had a negative effect on the total root length， surface area， volume， tips with a diameter 0-0.5 mm of L.olgensis seedlings under +M and -M treatments. The root morphology indexes of -M was higher than that of +M treatment under 0N， LN， and MN treatments， respectively. ②With the increasing of nitrogen deposition， the activities of BG， LAP， ACP， ALP in the rhizosphere soils showed a tendency of first rising and then decreasing under the two inoculation treatments， but the activities of NAG showed a decreasing tendency. ③The morphology of roots with diameter of 0-0.5 mm was significantly negatively correlated with the activities of BG in the rhizosphere soils（P<0.05）， under the +M and -M treatments. There was significantly positive correlation between the activities of NAG and root morphology（P<0.05）， except the number of the tips. The effect of nitrogen deposition on root morphology of L.olgensis seedlings was weakened by ectomycorrhizal fungi inoculation treatment. However， the activation of soil enzyme activity by +M was higher than that of -M treatment under low nitrogen deposition.

Key words: Larix olgensis, nitrogen deposition, ectomycorrhizal fungi, root morphology, soil enzyme activities

CLC Number:

S723.1

Ting-Yan LIU, Long-Fei HAO, Xu-Fu WANG, Hai-Xia YAN, Shu-Lan BAI. Effects of Nitrogen Deposition and Ectomycorrhizal Fungi on Root Architecture and Rhizosphere Soil Enzyme Activities of Larix olgensis Seedlings[J]. Bulletin of Botanical Research, 2021, 41(1): 145-151.

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