Genetic Transformation and Function Analysis of PsnHB13 Gene Isolated from Populu ssimonii×P.nigra in Nicotiana tabacum

doi:10.7525/j.issn.1673-5102.2020.04.014

Abstract

Abstract: Cloning of the gene PsnHB13 of the HD-ZIP family, bioinformatics analysis, the construction of overexpression vector and performing of tobacco genetic transformation. The result shows that the cDNA of PsnHB13 was 870 bp and encode 289 amino acids. In our study, PsnHB13 was amplified from Populu ssimonii×P.nigra cDNA by PCR. Plant overexpression vector(pROKII-PsnHB13) was constructed and transformed into wild-type tobacco by agrobacterium-mediated leaf disc transformation. The transgenic plants were confirmed by PCR, and the result showed PsnHB13 gene was integrated into tobacco genome. The result of qRT-PCR detection of T2 generation plants showed the mRNA of PsnHB13 was transcribed in transgenic tobaccos. By observation, PsnHB13 overexpressed leads the differences of leaf, flower and root in tobacco. Thus, PsnHB13 plays a negative role in the growth of tobacco leaves, roots and flowers, and provides a theoretical basis for poplar growth and development.

Key words: PsnHB13, tobacco, leaf shape, root length

CLC Number:

S792.11

XIAO Di, LIU Yi, LI Kai-Long, ZHENG Mi, QU Guan-Zheng. Genetic Transformation and Function Analysis of PsnHB13 Gene Isolated from Populu ssimonii×P.nigra in Nicotiana tabacum[J]. Bulletin of Botanical Research, 2020, 40(4): 593-601.

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