Allelic Variation in 4-coumarate:CoA ligase(4CL) Associated with Wood Properties of Larix olgensis

doi:10.7525/j.issn.1673-5102.2016.02.013

Abstract

Abstract: We used 10 provenances of Larix olgensis to study the effects of polymorphism of 4CL gene on wood quality and growth traits in L.olgensis. The polymorphism in 4CL gene was identified using DNA sequencing, 64 mean SNPs were identified,the median SNP frequency was one site per 31 bp,and the average nucleotide diversity for the sequenced regions was calculated to be π_T =0.015 77 and θ_W =0.008 78. The diversity level of synonymous nucleotide sbustituons(π_syn=0.022 56) was 1.4 of nonsynoymous nucleotide substitutions(π_nonsyn=0.015 75), suggesting that the gene misht be evolved under purifying selection at the synonoymous sites of the coding region in L.olgensis. The association between the 27 common SNPs(frequency>10%), haplotypes and wood quality and growth traits were analyzed respectively, indicating that 7 SNPs and 6 haplotypes were significantly associated with wood quality and growth traits, explaining between 0.09%-2.08% of the phenotypic variance. Ourstudy provided an important genetic foundation for molecular marker-assistant selection breeding programs with the goals of improving the wood quality and growth products in L.olgensis.

Key words: Larix olgensis, lignin, 4CL gene, SNPs, haplotype

WANG Yan-Hong1,2；JIA Qing-Bin1；ZHANG Lei1；ZHANG Han-Guo1*. Allelic Variation in 4-coumarate:CoA ligase(4CL) Associated with Wood Properties of Larix olgensis[J]. Bulletin of Botanical Research, 2016, 36(2): 242-251.

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