苜蓿体内H2S信号与Ca2+调节气孔运动的作用机制

doi:10.7525/j.issn.1673-5102.2023.02.013

植物研究 ›› 2023, Vol. 43 ›› Issue (2): 281-287.doi: 10.7525/j.issn.1673-5102.2023.02.013

苜蓿体内H₂S信号与Ca²⁺调节气孔运动的作用机制

郝雪峰¹, 亢春霞¹, 裴雁曦², 金竹萍²

^1.太原师范学院，生物科学与技术学院，晋中 030619
^2.山西大学，生命科学学院，特色植物资源研究与利用山西省重点实验室，太原 030006

收稿日期:2022-07-13 出版日期:2023-03-20 发布日期:2023-03-07
作者简介:郝雪峰（1978—），女，博士，副教授，主要从事非生物胁迫下植物气体信号转导研究。
基金资助:
国家自然科学基金项目(32172550);山西省回国人员项目(2020-014);山西省自然科学基金(20210302123431)

The Mechanism of H₂S Signal and Ca²⁺ Regulating Stomatal Movement in Medicago sativa

Xuefeng HAO¹, Chunxia KANG¹, Yanxi PEI², Zhuping JIN²

^1.College of Biological Sciences and Technology，Taiyuan Normal University，Jinzhong 030619
^2.Shanxi Key Laboratory for Research and Development of Regional Plants，School of Life Science，Shanxi University，Taiyuan 030006

Received:2022-07-13 Online:2023-03-20 Published:2023-03-07
About author:HAO Xuefeng（1978—），femal，doctor，associate professor， mainly engaged in research on plant gas signal transduction under abiotic stress.
Supported by:
National Natural Science Foundation of China(32172550);Shanxi Province Foundation for Returness(2020-014);Natural Science Foundation of Shanxi Province of China(20210302123431)

摘要/Abstract

摘要：

为探究H₂S信号在苜蓿（Medicago sativa）体内调节气孔运动的作用，及在此过程中H₂S与Ca²⁺的关系，以蒺藜苜蓿（Medicago truncatula）的野生型和钙离子转运体突变体为试验材料，分别从转录水平、细胞水平和生理水平开展研究。采用qRT-PCR比较相关基因的表达量变化、荧光探针显示体内Ca²⁺含量、电极法测定H₂S含量、光学显微镜观察和测量气孔孔径等。结果表明：蒺藜苜蓿突变体NF3011和NF2734体内H₂S的含量与野生型相比极显著降低（P<0.01）；H₂S信号在一定程度上抑制钙离子转运体编码基因MTR_6g027580的表达；外源生理浓度H₂S熏蒸可诱导蒺藜苜蓿气孔关闭，与Ca²⁺通道阻断剂LaCl₃联合处理对野生型气孔运动未产生影响，而在突变体中的结果截然相反；利用荧光探针测定保卫细胞内的Ca²⁺含量，所得结果与气孔孔径的变化规律完全一致。综上所述，H₂S信号促进叶片保卫细胞内Ca²⁺的含量增加，最终表现为植物气孔孔径变小，在此过程中胞内Ca²⁺含量变化主要通过Ca²⁺转运体进行，少部分依赖Ca²⁺离子通道。该研究结果不仅在理论上丰富了H₂S信号的作用机制，更具应用于苜蓿生产实践并推广于其他作物的潜力。

关键词: H₂S信号, Ca²⁺, 气孔运动, 蒺藜苜蓿

Abstract:

To explore the role of H₂S signal in regulating stomatal movement in alfalfa and the relationship between H₂S and Ca²⁺ during this process， wild type and the calcium transporter mutants of Medicago truncatula were used as experimental materials， and the transcriptional level， cellular level and physiological level were studied respectively， and the expression levels of related genes were compared by qRT-PCR， the content of Ca²⁺in vivo was detected by fluorescent probe， the content of H₂S was measured by electrode method， and the stomatal aperture was observed by microscope respectively. The results showed that compared with the wild type， the content of H₂S in the mutant NF3011 and NF2734 decreased significantly； the H₂S signal inhibited the expression of calcium transporter encoding gene MTR_6g027580， the exogenous physiological concentration of H₂S fumigation could induce stomatal closure of Medicago truncatula， and the combination of treatment with LaCl₃， Ca²⁺ channel blocker， had no effect on stomatal movement of wild type， but opposite in mutant； the concentration of Ca²⁺ in guard cells was measured by fluorescence probe， which were consistent with the change law of stomatal aperture. In conclusion， the content of Ca²⁺ in guard cells of leaves is induced by H₂S， whose stomatal aperture become smaller at the same time. In this process， the change of intracellular Ca²⁺ concentration mainly depend on Ca²⁺ transporter， and small partly depend on Ca²⁺ channel. The results of this study not only enrich the mechanism of H₂S signaling in theory， but also have the potential to be applied to alfalfa production and other crops.

Key words: H₂S signal, Ca²⁺ transporter, stomatal movement, Medicago truncatula

中图分类号:

Q945.78

郝雪峰, 亢春霞, 裴雁曦, 金竹萍. 苜蓿体内H₂S信号与Ca²⁺调节气孔运动的作用机制[J]. 植物研究, 2023, 43(2): 281-287.

Xuefeng HAO, Chunxia KANG, Yanxi PEI, Zhuping JIN. The Mechanism of H₂S Signal and Ca²⁺ Regulating Stomatal Movement in Medicago sativa[J]. Bulletin of Botanical Research, 2023, 43(2): 281-287.

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苜蓿体内H₂S信号与Ca²⁺调节气孔运动的作用机制

The Mechanism of H₂S Signal and Ca²⁺ Regulating Stomatal Movement in Medicago sativa

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