长白落叶松4CL基因单核苷酸多态性及其与木材材性的关联分析

doi:10.7525/j.issn.1673-5102.2016.02.013

摘要/Abstract

摘要： 为探讨长白落叶松4CL基因多态性与木材材性及生长性状的关系，本研究以10个种源的长白落叶松为实验材料，采用直接测序方法开展4CL基因多态性检测，共检测到64个SNPs，SNP频率为1/31 bp，多样性指数π_T值为0.015 77，θ_W值为0.008 78。同义突变核苷酸多样性（π_syn=0.022 56）是非同义突变核苷酸多样性（π_nonsyn=0.015 75）的1.4倍，推测长白落叶松4CL基因编码区在长白落叶松物种演化过程中受到纯化选择压力的作用。对频率大于10%的27个常见SNP的单位点和单倍型分别于长白落叶松木材材性和生长性状进行关联分析，检测到7个SNPs和6个单倍型的与木材材性和生长性状显著关联，表型贡献率为0.09%~2.08%。研究结果为基于分子标记的长白落叶松优良木材品质及生长性状定向辅助育种提供理论依据。

关键词: 长白落叶松, 木质素, 4CL基因, SNPs, 单倍型

Abstract: We used 10 provenances of Larix olgensis to study the effects of polymorphism of 4CL gene on wood quality and growth traits in L.olgensis. The polymorphism in 4CL gene was identified using DNA sequencing, 64 mean SNPs were identified,the median SNP frequency was one site per 31 bp,and the average nucleotide diversity for the sequenced regions was calculated to be π_T =0.015 77 and θ_W =0.008 78. The diversity level of synonymous nucleotide sbustituons(π_syn=0.022 56) was 1.4 of nonsynoymous nucleotide substitutions(π_nonsyn=0.015 75), suggesting that the gene misht be evolved under purifying selection at the synonoymous sites of the coding region in L.olgensis. The association between the 27 common SNPs(frequency>10%), haplotypes and wood quality and growth traits were analyzed respectively, indicating that 7 SNPs and 6 haplotypes were significantly associated with wood quality and growth traits, explaining between 0.09%-2.08% of the phenotypic variance. Ourstudy provided an important genetic foundation for molecular marker-assistant selection breeding programs with the goals of improving the wood quality and growth products in L.olgensis.

Key words: Larix olgensis, lignin, 4CL gene, SNPs, haplotype

王艳红1,2；贾庆斌1；张磊1；张含国1*. 长白落叶松4CL基因单核苷酸多态性及其与木材材性的关联分析[J]. 植物研究, 2016, 36(2): 242-251.

WANG Yan-Hong1,2；JIA Qing-Bin1；ZHANG Lei1；ZHANG Han-Guo1*. Allelic Variation in 4-coumarate:CoA ligase(4CL) Associated with Wood Properties of Larix olgensis[J]. Bulletin of Botanical Research, 2016, 36(2): 242-251.

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