木霉诱导下山新杨PodaPIN9基因的组织表达调控

doi:10.7525/j.issn.1673-5102.2019.02.013

摘要/Abstract

摘要： PIN蛋白具有多个跨膜结构域，影响着高等植物生长素的外向运输和众多生长发育过程。木霉菌是能促进植物生长、提高其对多种病害防御作用的生防因子。研究木霉菌对木本植物山新杨生长素的极性分布的影响有重要意义。克隆了山新杨PodaPIN9基因，对其核酸和蛋白序列进行分析；同时，构建的进化树显示PodaPIN9与6个物种的9条PIN基因具有高度一致性（>80%）。qRT-PCR分析表明，PodaPIN9在山新杨茎尖、成熟叶和根中均有表达。在根中表达量极低；在茎尖和叶中的表达量极高，分别是根中的503和346倍。该基因受木霉影响在茎尖和叶中的表达量均显著下调；而根中的表达量显著上调，达到对照的32.01倍。并发现根际接种木霉48 h会使杨树茎尖、叶和根中生长素含量降低。说明木霉菌能影响杨树茎尖、叶和根中IAA水平及PodaPIN9的表达量。并且Pearson相关性分析表明在茎尖、叶和根中，PodaPIN9表达量与IAA水平具有不同的相关性。

关键词: 山新杨, 棘孢木霉菌, PIN基因, qRT-PCR, IAA

Abstract: The PIN proteins contain multiple transmembrane domains, and are involved in the efflux of auxin, affecting many growth and development-related processes in higher plants. Trichoderma is a biocontrol agent that promotes the growth and improves the defense mechanisms of plants against various diseases. Therefore, it is important to study the effects of Trichoderma on the distribution of auxin in the woody species Populus davidiana×P.alba var. pyramidlis(Shanxin poplar). In this study, the gene PodaPIN9 was cloned from Shanxin poplar, and its nucleotide and protein sequences were analyzed. The constructed phylogenetic tree showed that PodaPIN9 has a high identity with 9 PIN genes from 6 species(>80%). By the qRT-PCR analysis, PodaPIN9 was expressed in the stem tips, mature leaves, and roots of the plant. The expression of PodaPIN9 was significantly low in the roots, but was significantly high in the stem tips and leaves(503 and 346 times higher than that in the roots, respectively). Following inoculation with Trichoderma, the expression of PodaPIN9 was significantly downregulated in the stem tips and leaves, whereas its expression was significantly upregulated in the roots. The expression of PodaPIN9 in the roots was 32.01 times higher than the control group. Additionally, we found that the levels of auxin in the stem tips, leaves, and roots of the plant were decreased at 48 h after inoculating the Trichoderma. It demonstrated that Trichoderma can affect auxin levels and the expression of PodaPIN9 in the stem tips, leaves, and roots of poplar. Furthermore, by Pearson correlation analysis, the correlation between the expression of PodaPIN9 and auxin levels differed in the stem tips, leaves, and roots.

Key words: Populus davidiana×P.alba var. pyramidlis, trichoderma asperellum, PIN, qRT-PCR, IAA

中图分类号:

S792.114

李枢航, 苗蕊, 常媛, 李俊男, 燕晓杰, 刘照莹, 张荣沭. 木霉诱导下山新杨PodaPIN9基因的组织表达调控[J]. 植物研究, 2019, 39(2): 267-275.

LI Shu-Hang, MIAO Rui, CHANG Yuan, LI Jun-Nan, YAN Xiao-Jie, LIU Zhao-Ying, ZHANG Rong-Shu. Differential Expression of PodaPIN9 Gene in Tissues of Populus davidiana×P.alba var. pyramidlis Induced by Trichoderma[J]. Bulletin of Botanical Research, 2019, 39(2): 267-275.

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