Genetic structure and demographic history of Cycas chenii (Cycadaceae), an endangered species with extremely small populations

doi:10.1016/j.pld.2016.11.003

Plant Diversity ›› 2017, Vol. 39 ›› Issue (01): 44-51.DOI: 10.1016/j.pld.2016.11.003

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Genetic structure and demographic history of Cycas chenii (Cycadaceae), an endangered species with extremely small populations

Rui Yang^a,b,c, Xiuyan Feng^a,b,c, Xun Gong^a,b,d

a. Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China;
b. Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China;
c. University of Chinese Academy of Sciences, Beijing 100049, China;
d. Yunnan Key Laboratory for Wild Plant Resources, Kunming 650201, China

Received:2016-05-09 Revised:2016-11-14 Online:2021-11-05 Published:2017-02-25
Contact: Xun Gong
Supported by:
This research was supported by the United Fund of the NSFC and the Yunnan Natural Science Foundation (Grant No. U1136602 to X. G.). We thank Wei Zhou, Jian Liu and Meng-meng Guan for their assistance with field sampling.

Genetic structure and demographic history of Cycas chenii (Cycadaceae), an endangered species with extremely small populations

Rui Yang^a,b,c, Xiuyan Feng^a,b,c, Xun Gong^a,b,d

a. Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China;
b. Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China;
c. University of Chinese Academy of Sciences, Beijing 100049, China;
d. Yunnan Key Laboratory for Wild Plant Resources, Kunming 650201, China

通讯作者: Xun Gong
基金资助:
This research was supported by the United Fund of the NSFC and the Yunnan Natural Science Foundation (Grant No. U1136602 to X. G.). We thank Wei Zhou, Jian Liu and Meng-meng Guan for their assistance with field sampling.

Abstract

Abstract: Geological activities and climate oscillations during the Quaternary period profoundly impacted the distribution of species in Southwest China. Some plant species may be harbored in refugia, such as the dry-hot valleys of Southwest China. Cycas chenii X. Gong & W. Zhou, a critically endangered cycad species, which grows under the canopy in subtropical evergreen broad-leaved forests along the upstream drainage area of the Red River, is endemic to this refugium. In this study, 60 individuals of C. chenii collected from six populations were analyzed by sequencing two chloroplast intergenic spacers (cpDNA: psbA-trnH and trnL-trnF) and two nuclear genes (PHYP and RBP-1). Results showed high genetic diversity at the species level, but low within-population genetic diversity and high interpopulation genetic differentiation. A Bayesian phylogenetic tree based on cpDNA showed that five chloroplast haplotypes were clustered into two clades, which corresponds to the division of the western and eastern bank of the Red River. These data indicate a possible role for the Red River as a geographic barrier to gene flow in C. chenii. Based on our findings, we propose appropriate in situ and ex situ conservation strategies for C. chenii.

Key words: Cycas chenii, Genetic variation, Phylogeography, Conservation

摘要： Geological activities and climate oscillations during the Quaternary period profoundly impacted the distribution of species in Southwest China. Some plant species may be harbored in refugia, such as the dry-hot valleys of Southwest China. Cycas chenii X. Gong & W. Zhou, a critically endangered cycad species, which grows under the canopy in subtropical evergreen broad-leaved forests along the upstream drainage area of the Red River, is endemic to this refugium. In this study, 60 individuals of C. chenii collected from six populations were analyzed by sequencing two chloroplast intergenic spacers (cpDNA: psbA-trnH and trnL-trnF) and two nuclear genes (PHYP and RBP-1). Results showed high genetic diversity at the species level, but low within-population genetic diversity and high interpopulation genetic differentiation. A Bayesian phylogenetic tree based on cpDNA showed that five chloroplast haplotypes were clustered into two clades, which corresponds to the division of the western and eastern bank of the Red River. These data indicate a possible role for the Red River as a geographic barrier to gene flow in C. chenii. Based on our findings, we propose appropriate in situ and ex situ conservation strategies for C. chenii.

关键词: Cycas chenii, Genetic variation, Phylogeography, Conservation

Rui Yang, Xiuyan Feng, Xun Gong. Genetic structure and demographic history of Cycas chenii (Cycadaceae), an endangered species with extremely small populations[J]. Plant Diversity, 2017, 39(01): 44-51.

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Genetic structure and demographic history of Cycas chenii (Cycadaceae), an endangered species with extremely small populations

Genetic structure and demographic history of Cycas chenii (Cycadaceae), an endangered species with extremely small populations

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