Integrative Biology Journals

Plant Diversity ›› 2015, Vol. 37 ›› Issue (2): 141-144.DOI: 10.7677/ynzwyj201514063

• Articles • Previous Articles     Next Articles

Development and Characterization of Microsatellite Loci for Heavenly Bamboo (Nandina domestica)

 HU  Hui-Li, LI  Bo, HU  Wan, SUN  Zhi-Xia, ZHANG  Zhi-Yong, FAN  Deng-Mei   

  1. Laboratory of Subtropical Biodiversity, Jiangxi Agricultural University, Nanchang 330045, China
  • Received:2014-04-10 Online:2014-07-20 Published:2015-03-25
  • Supported by:

    National Natural Science Foundation of China (grant nos. 31160043 and 31160082) and National Science and Technology Support Program (2012BAC11B02)

南天竹微卫星标记开发及特性分析

 呼慧丽, 李波, 胡菀, 孙志霞, 张志勇, 范邓妹   

  1. 江西农业大学亚热带生物多样性实验室,南昌330045
  • 基金资助:

    National Natural Science Foundation of China (grant nos. 31160043 and 31160082) and National Science and Technology Support Program (2012BAC11B02)

Abstract:

We developed and characterized a total of 10 novel polymorphic microsatellite loci from the genome of an ornamental shrub, Nandina domestica. The number of alleles per locus in 24 individuals from four populations varied from 2 to 6. The expected and observed heterozygosity ranged from 0153 to 0778 and from 0 to 1, respectively. These markers will provide tools for future studies on population genetic diversity of Ndomestica. Additionally, most of our new markers could yield the amplification products in four other Berberidaceae species.

Key words: Nandina domestica, Microsatellite marker, Genetic diversity

摘要:

:南天竹 (Nandina domestica) 是一种具有较高观赏价值的常绿灌木。本研究从南天竹基因组中开发和筛选出10个微卫星位点,能在该物种中进行稳定PCR扩增且具一定的多态性。采用4个南天竹群体24个个体进行检测发现,每个位点的等位基因数为2~6,期望杂合度和表观杂合度分别为0153~0778和0~1。本研究结果为后续对南天竹遗传多样性的研究打下了基础。另外,这10个位点在小檗科4个其他物种中也有一定的通用性。

关键词: 南天竹, 微卫星, 遗传多样性

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