Abstract:

We developed and characterized a total of 10 novel polymorphic microsatellite loci from the genome of an ornamental shrub, Nandina domestica. The number of alleles per locus in 24 individuals from four populations varied from 2 to 6. The expected and observed heterozygosity ranged from 0153 to 0778 and from 0 to 1, respectively. These markers will provide tools for future studies on population genetic diversity of Ndomestica. Additionally, most of our new markers could yield the amplification products in four other Berberidaceae species.

Key words: Nandina domestica, Microsatellite marker, Genetic diversity

摘要：

：南天竹 （Nandina domestica） 是一种具有较高观赏价值的常绿灌木。本研究从南天竹基因组中开发和筛选出10个微卫星位点，能在该物种中进行稳定PCR扩增且具一定的多态性。采用4个南天竹群体24个个体进行检测发现，每个位点的等位基因数为2~6，期望杂合度和表观杂合度分别为0153~0778和0~1。本研究结果为后续对南天竹遗传多样性的研究打下了基础。另外，这10个位点在小檗科4个其他物种中也有一定的通用性。

关键词: 南天竹, 微卫星, 遗传多样性