Study on the Sampling Strategy of Sinocalycanthus chinensis for Exsitu Conservation of Genetic Diversity

Abstract

Abstract:

By analysis of genetic diversity and population genetic structure in natural populations of Sinocalycanthus chinensis with intersimple sequence repeat(ISSR) technique, the sampling strategy were determined based on the gene capture curve of 95% allele captured. The results are as follows: 6 populations to be sampled, the sampling proportion should be 30% and the distance between the sampled individuals are best of 26 m above. After seeds sampling and conduction of Exsitu conservation, the genetic diversity of the offspring population were evaluated that it conserved the genetic diversity of the natural populations effectively. Therefore, the systemic sampling method of endangered plants for Exsitu conservation of genetic diversity were discussed.

Key words: Sinocalycanthus chinensis, Genetic diversity, Exsitu conservation, Sampling strategy

摘要：

采用ISSR分子标记技术分析夏蜡梅天然群体的遗传多样性及空间遗传结构并在此基础上利用计算机模拟取样，以保留95%以上天然群体等位基因为标准制定出夏蜡梅遗传多样性的取样策略，即采集6个群体，群体抽样比例为30%，居群取样时应适当控制采种间距在26 m以上。采种实施迁地保护后，后代群体检测证实该取样策略能够有效保留原始群体的遗传多样性，由此探讨了濒危植物系统迁地保护的采样模式与技术措施。

关键词: 夏蜡梅, 遗传多样性, 迁地保护, 取样策略

CLC Number:

Q 16

CHEN Xiang-Bo-, TIAN Qi. Study on the Sampling Strategy of Sinocalycanthus chinensis for Exsitu Conservation of Genetic Diversity[J]. Plant Diversity, 2010, 32(S17): 74-80.

陈香波1, 田旗2. 夏蜡梅遗传多样性迁地保护取样策略研究[J]. Plant Diversity, 2010, 32(S17): 74-80.

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