Abstract: Taxus chinensis cell cultures were extracted with methanol and then prepared by solid-phase extract prior to HPLC or LC-MS analysis . About 13 taxane compounds were identified or characterized by comparisons with LC-MS and standard referents . The profiling analysis of the taxanes by methyl jasmonate (MJ ) elicitation indicated that the relative chromatographic peak areas of all these identified compounds were increased but the increasing rates differed . Taxchinin M and its structural analogue had the highest increment . The increasing rates of taxol, B -Ⅲ and B -Ⅵ were more than that of taxuyunnanine C and its derivatives, which were oxygenated at C-14 . The results suggested that biosynthsis of taxol and its precursors were more sensitive than oxygenated taxanes at C-14 in response to methyl jasmonate elicitations, while MJ may also elicit the formation of non-effective acetylated taxanes which bear acetate groups at C1 ,C2 , C7 , C9 and C13 . The study may be interesting in metabolic profiling analysis for elucidating taxane biosynthesis .

Key words: Taxanes

摘要： 红豆杉细胞培养物经甲醇提取和固相萃取粗分离, 进行反相高效液相色谱分析, 获得了约含13 个与紫杉醇极性相关的化合物色谱图, 通过质谱联用和对照品参照, 归属了这些化合物组成。进一步利用茉莉酸甲酯(MJ) 诱导细胞, 比较这些组分在诱导前后的相对色谱峰面积变化, 结果表明, 所有紫杉烷组分的浓度在诱导后都提高, 其中以taxchinin M及其结构类似物提高最显著, 紫杉醇, B - Ⅲ和B - Ⅵ等比C14 位取代的taxuyunnanine C 及其衍生物的浓度增加幅度要大, 提示MJ 对紫杉醇合成中非有效乙酰化旁路代谢有促进作用, 而对C14 位取代物生成的旁路促进作用不明显。本文为紫杉醇的生物合成研究提供了新的思路和方法。

关键词: 紫杉烷, 代谢轮廓分析, 红豆杉细胞培养物, 茉莉酸甲酯诱导FONT