The Relationship between Aleuritopteris humatifolia and A-chrysophylla Based on Three Chloroplast DNA Sequences Analyses

doi:10.7677/ynzwyj201514127

Abstract

Abstract:

Total DNA from 27 species including Aleuritopteris humatifolia and Achrysophylla and related taxa was extracted in this research, and the rbcL, trnLF and matK sequences were amplified and sequenced. The sequences were aligned by ClustalX. The phylogenetic trees were constructed by Maximum parsimony methods and the intraspecific and interspecific genetic distance were computed in MEGA 60. The results showed as follows: no significant difference among rbcL, trnLF and matK sequences of one sample of Ahumatifolia and two samples of Achrysophy
lla, and the maximum genetic distance between Ahumatifolia and Achrysophylla were less than the minimum genetic distance between Ahumatifolia and other closely related species. Therefore, our study support the taxonomic treatment of reducing Ahumatifolia to synonym of Achrysophylla based on our phylogenetic analysis combining the evidences from their morphological characters of blades, spores, indusia, and rhizome scales.

Key words: Aleuritopteris humatifolia, A-chrysophylla, cheilanthoid ferns, rbcL, trnL-F, matK

摘要：

选择阴石粉背蕨（Aleuritopteris humatifolia）和金粉背蕨（Achrysophylla）及其相关类群共27种植物，提取基因组DNA，通过聚合酶链式反应（PCR）扩增其叶绿体rbcL，trnLF和matK序列，所得序列利用 ClustalX 软件进行对位排列，用 MEGA60软件计算种内及种间遗传距离，并采用最大简约法（MP）构建系统发育树。结果显示：阴石粉背蕨1个样品和金粉背蕨2个样品的rbcL，trnLF和matK序列间差异很小，最大遗传距离均小于其与其它近缘种的种间最小遗传距离，结合两个种叶片形态、孢子、囊群盖和根状茎鳞片等形态学特征的对比研究，作者认为阴石粉背蕨应该作为金粉背蕨的异名处理。

关键词: 阴石粉背蕨, 金粉背蕨, 碎米蕨类, rbcL, trnL-F, matK

CLC Number:

Q 75， Q 949

WANG Wan, YANG Wen-Li, DOU Ping, ZHAO Dan-Dan, ZHANG Gang-Min. The Relationship between Aleuritopteris humatifolia and A-chrysophylla Based on Three Chloroplast DNA Sequences Analyses[J]. Plant Diversity, 2015, 37(03): 301-308.

王弯, 杨文利, 窦平, 赵冉冉, 张钢民. 基于三种叶绿体基因序列的证据分析阴石粉背蕨和金粉背蕨的关系[J]. Plant Diversity, 2015, 37(03): 301-308.

References

秦仁昌, 邢公侠, 1990. 中国植物志3卷 (1)［M］. 北京: 科学出版社
张钢民, 2003. 中国碎米蕨类及其相关类群的系统学研究［D］ (博士论文). 北京: 中国科学院
Ching RC, 1941. The studies of Chinese ferns, Part XXXI［J］. Hong Kong Naturalist (香港博物学杂志), Vol. X: 194—204
Christenhusz MJM, Zhang XC, Schneider H, 2011. A linear sequence of extant families and genera of lycophytes and ferns［J］. Phytotaxa, 19: 7—54
Copeland EB, 1947. Genera Filicum［M］. Waltham, Mass achusetts: Chronica Botanica Co, 65—82
Doyle JJ, Doyle JL,1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue［J］. Phytochemical Bulletin, 19: 11—15
FraserJenkins CR, 1997. New Species Syndrome in Indian Pteridology and the Ferns of Nepal［M］. Dehra Dun (India): International Book Distributors
Gou CY (勾彩云), Zhang SZ (张寿洲), Geng SL (耿世磊), 2008. Phylogenetic Position and Genetic Relationship of Osmunda mildei (Osmundaceae): Evidence from rbcL Gene and trnLtrnF Region［J］. Acta Botanica BorealiOccidentalia Sinica (西北植物学报), 28 (11): 2178—2183
Hennequin S, Ebihara A, Ito M et al., 2003. Molecular systematics of the fern genus Hymenophyllum sl. (Hymenophyllaceae) based on chloroplastic coding and noncoding regions［J］. Molecular Phylogenetics and Evolution, 27: 283—301
Kimura M, 1981. Estimation of evolutionary distances between homologous nucleotide sequences［J］. Journal of Molecular Evolution, 16: 111
Koichiro T, Glen S, Daniel P et al., 2013. MEGA6: Molecular Evolutionary Genetics Analysis version 60［J］. Molecular Biology and Evolution, 30: 2725—2729
Kuo LY, Li FW, Chiou WL et al., 2011. First insights into fern matK phylogeny［J］.Molecular Phylogenetics and Evolution, 59: 556—566
Li CX, Lu SG, Yang Q, 2004. Asian origin for Polystichum (Dryopteridaceae) based on rbcL sequences［J］. Chinese Science Bulletin, 49: 1146—1150
Liu HM, Wang L, Zhang XC et al., 2008. Advances in the studies of lycophytes and monilophytes with reference to systematic arrangement of families distributed in China［J］. Journal of Systematics Evolution, 46 (6): 808—829
Lu JM, Li DZ, Gao LM et al., 2005. Paraphyly of Cyrtomium (Dryopteridaceae): Evidence from rbcL and trnLF sequence data［J］. Journal of Plant Research, 118: 129—135
Schuettpelz E, Pryer KM, 2007. Fern phylogeny inferred from 400 leptosporangiate species and three plastid genes［J］. Taxon, 56: 1037—1050
Schuettpelz E, Schneider H, Huiet L et al., 2007. A molecular phylogeny of the fern family Pteridaceae: Assessing overall relationships and the affinities of previously unsampled genera［J］. Molecular Phylogenetics and Evolution, 44: 1172—1185
Skog JE, Mickel JT, Moran RC et al., 2004. Molecular studies of representative species in the fern genus Elaphoglossum (Dryopteridaceae) based on chloroplast DNA sequences rbcL, trnLF, and rps4trnS［J］. International Journal of Plant Sciences, 165: 1063—1075
Smith AR, Pryer KM, Schuettpelz E et al., 2006. A classification for extant ferns［J］. Taxon, 55: 705—731
Taberlet P, Gielly L, Pautau G et al., 1991. Universal primers for amplification of 3 noncoding regions of chloroplast DNA［J］. Plant Molecular Biology, 17 (5): 1105—1109
Thompson JD, Gibson TJ, Plewniak F et al., 1997. The ClustaX windows interface: flexible strategies for multiple sequences alignment aided by quality analysis tools［J］. Nuclear Acids Research, 24: 4876—4882
Trewick SA, MorganRichards M, Russell SJ et al., 2002. Polyploidy phylogeography and Pleistocene refugia of the rockfern Asplenium ceterach: evidence from chloroplast DNA［J］. Molecular Ecology, 11: 2003—2012
Tryon RM, Tryon AF, 1973. Geography, spores and evolutionary relations in the cheilanthoid ferns［A］// Jermy AC et al. eds., The Phylogeny and Classification of the Ferns［M］. London: Academic Press, 145—153
Tryon RM, Tryon AF, 1982. Ferns and Allied Plants with Special Reference to Tropical America［M］. New York: SpringerVerlag, 213—354
Wang L, Wu ZH, Xiang QP et al., 2010. A molecular phylogeny and a revised classification of tribe Lepisoreae (Polypodiaceae) based on an analysis of four plastid DNA regions［J］. Botanical Journal of the Linnean Society, 162: 28—38
Windham MD, Huiet L, Schuettpelz E et al., 2009. Using plastid and nuclear DNA sequences to redraw generic boundaries and demystify species complexes in cheilanthoid ferns［J］. American Fern Journal, 99: 128—132
Wu SK (武素功), 1979. Leptolepidium—A new genus of Sinopteridaceae［J］. Acta Botanica Yunnanica（云南植物研究）, 1 (1): 115—118
Wu SK (武素功), 1981. The studies of Chinese Aleuritopteris［J］. Acta Phytotaxonomica Sinica (植物分类学报), 19 (1): 57—74
Yu J (于晶), Wang QX (王全喜), Bao WM (包文美), 2001. Spore morphology of pteridophytes from China Sinopteridaceae［J］. Acta Phytotaxonomica Sinica (植物分类学报), 39 (3): 224—233
Zhang GM, Yatskievych G, 2013. Cheilanthoideae［A］// Wu ZY, Raven PH eds., Flora of China Vol2-3［M］. Beijing: Science Press, St. Louis: Missouri Botanical Garden Press, 216—237
Zhang GM, Zhang XC, Chen ZD et al., 2007. First insights in the phylogeny of Asian cheilanthoid ferns based on sequences of two chloroplast markers［J］. Taxon, 56: 369—378
Zhang XC (张宪春), Shi L (石雷), 1994. Notes on Aleuritopteris fern in China［J］. Acta Phytotaxonomica Sinica (植物分类学报), 32 (1): 93—97

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